Meng Wan
(Department of Chemical Engineering and Polymer Science, Yanbian University, Department of Polymer Science, Kyungpook National University)
,
Jung Kyung-Hye
(Department of Polymer Science, Kyungpook National University)
,
Kang Inn-Kyu
(Department of Polymer Science, Kyungpook National University)
,
Kwon Oh Hyeong
(Department of Polymer Science & Engineering, Kumoh National Institute of Technology)
,
Akaike Toshihiro
(Department of Biomolecular Engineering, Tokyo Institute of Technology)
Polyurethanes containing z-Iysine segments in the main chain (PULL) were synthesized from 4,4'-diphe-nylmethyl diisocyanate, poly(tetramethylene glycol), and z-Iysine oligomer as a chain extender. The PULL film was treated first with a $10\%$ HBr-acetic acid solution and subsequently with...
Polyurethanes containing z-Iysine segments in the main chain (PULL) were synthesized from 4,4'-diphe-nylmethyl diisocyanate, poly(tetramethylene glycol), and z-Iysine oligomer as a chain extender. The PULL film was treated first with a $10\%$ HBr-acetic acid solution and subsequently with a saturated sodium bicarbonate aqueous solution to produce a primary amine group on the surface (PULL-N). Lactobionic acid (LA)-immobilized PULL (PULL-L) was prepared by the coupling reaction of the PULL surface amine groups and the LA carboxylic acid groups. The surface-modified PULLs were then characterized by attenuated total reflection-Fourier transform infra-red spectroscopy, electron spectroscopy for chemical analysis, atomic force microscopy, and contact angle goniometry. In the hepatocytes adhesion experiment, the cells poorly adhered to the PULL surface, although they adhered moderately well to the PULL-N surface. On the other hand, the cells adhered well to the PULL-L surface, suggesting the good affinity of the surface $\beta$-galactose moieties for hepatocytes. When hepatocytes were cultured in the presence of epidermal growth factor for 48 h, the cells rapidly aggregated on the PULL-L surface, whereas they aggregated only slowly on the other surfaces. The PULL prepared in this study has the potential to be used as a coating material for the enhancement of hepatocyte adhesion.
Polyurethanes containing z-Iysine segments in the main chain (PULL) were synthesized from 4,4'-diphe-nylmethyl diisocyanate, poly(tetramethylene glycol), and z-Iysine oligomer as a chain extender. The PULL film was treated first with a $10\%$ HBr-acetic acid solution and subsequently with a saturated sodium bicarbonate aqueous solution to produce a primary amine group on the surface (PULL-N). Lactobionic acid (LA)-immobilized PULL (PULL-L) was prepared by the coupling reaction of the PULL surface amine groups and the LA carboxylic acid groups. The surface-modified PULLs were then characterized by attenuated total reflection-Fourier transform infra-red spectroscopy, electron spectroscopy for chemical analysis, atomic force microscopy, and contact angle goniometry. In the hepatocytes adhesion experiment, the cells poorly adhered to the PULL surface, although they adhered moderately well to the PULL-N surface. On the other hand, the cells adhered well to the PULL-L surface, suggesting the good affinity of the surface $\beta$-galactose moieties for hepatocytes. When hepatocytes were cultured in the presence of epidermal growth factor for 48 h, the cells rapidly aggregated on the PULL-L surface, whereas they aggregated only slowly on the other surfaces. The PULL prepared in this study has the potential to be used as a coating material for the enhancement of hepatocyte adhesion.
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제안 방법
Survey scan spectra were taken and surface elemental compositions relative to carbon were calculated from peak heights with a correction for atomic sensitivity.20 Surfaces of modified PULLs were investigated using a Midac spectrophotometer equipped with a ZnSe reflection element. The water contact angles of the modified surfaces were also measured at room temperature using a contact angle goniometer (model 100-0, Rame- Hart, NJ, USA) to evaluate the wettability of the samples.
The surface-modified PULLs were analyzed using ESCA (ESCALAB MKⅡ, V.G. Scientific Co., East Grinstead, UK) equipped with AlKcr at 1487 eV and 300 W power at the anode. Survey scan spectra were taken and surface elemental compositions relative to carbon were calculated from peak heights with a correction for atomic sensitivity.
The water contact angles of PULLs in air were slightly greater than those in water. To study the surface morphologies of PULL, PULL-N, and PULL-L, an atomic force microscope image was examined using a tapping mode and expressed as phase images. On the PULL surface, a relatively homogeneous pattern was observed as shown in Figure 5.
이론/모형
1 wt% Triton X-100 aqueous solution in an ultrasonic cleaner filled with distilled water, and finally dried under reduced pressure for 12 h at room temperature. After immobilization of lactobionic acid on the PULL-N, the non-reacted amine groups (1.5 /anolcrn'2) on PULL-L film surface was determined by fluorescamine method. As the result, the concentration of immobilized lactobionic acid on the PULL film was 0.
, Korea) by the modified collagenase perfusion technique of Seglen.14 The viability of the isolated hepatocytes was determined by the trypan blue exclusion method. Hepatocytes with higher than 90% viability were used for the following experiments.
성능/효과
Yang et al?5 prepared gelatin-immobilized polyurethane foams and cultured rat hepatocytes on their surfaces. The results showed that the amount of hepatocytes seeded on PUFG1, prepared by using a 1% aqueous gelatin solution, was higher than that on other PUFGs. In the current study, lactobionic acids (LA) were covalently coupled with primary amine groups generated on the PULL surface.
참고문헌 (39)
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