[논문]Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea

Nam, Jin-Sik

[국내논문] Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea 원문보기

환경생물 = Korean journal of environmental biology, v.23 no.4, 2005년, pp.335 - 342

Nam, Jin-Sik (Department of Food & Nutrition, Faculty of Food Science, Suwon Women's College)

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We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.

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참고문헌 (26)

Bowen AR, JL Chen-Wu, M Momany, R Young, PJ Szaniszlo and PW Robbins. 1992. Classification of fungal chitin synthases. Proc. Natl. Acad. Sci. USA 89:519-523
Cabib E, A Sburlati, B Bowers and SJ Silverman. 1989. Chitin synthase 1, an auxiliary enzyme for chitin synthesis in Saccharomyces cerevisiae. J. Cell Biol. 108:1665-1672

상세보기
Cabib E, B Bower, A Sburlati and SJ Silverman. 1988. Fungal cell wall synthesis: the construction of a biological structure. Microbiol. Sci. 5:370-375

상세보기
Cabib E, SJ Silverman and JA Shaw. 1992. Chitinase and chitin synthase 1: counterbalancing activities in cell separation of Saccharomyces cerevisiae. J. Gen. Microbiol. 138:97-102

상세보기
Cho SP, SK Lee, DH Lee, KS Bae, HM Park and PJ Maeng. 1997. Cloning of two chitin synthase gene fragments from Penicillium diversum. J. Microbiol. 25:167-175
Fell JW, T Boekhout, A Fonseca, G Scorzetti and A Statzell-Tallman. 1998. Validation of the basidiomycetous yeast, Sporidiobolus microsporus sp. nov., based on phenotypic and molecular analyses. Antonie Leeuwenhoek 74:265-270

상세보기
Felsenstein J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5c. Department of Genetics, University of Washington, Seattle
Golubev WI. 1999. Mastigobasidium, a new telemorphic genus for the perfect state of ballistosporous yeast Bensingtonia intermedia. Int. J. Sys. Bacteriol. 49:1301-1305

상세보기
Hirai A, R Kano, Y Nakamura, S Watanabe and A Hasegawa. 2003. Molecular taxonomy of dermatophytes and related fungi by chitin synthase 1 (CHSI) gene sequences. Antonie Leeuwenhoek 83:11-20
Hong SG, J Chun, JS Nam, YD Park and KS Bae. 2000. Phylogenetic analysis of genus Sporobolomyces based on partial sequences of 26S rDNA. J. Microbiol. Biotechnol. 10:363-366

원문보기 상세보기
Hong SG, KH Lee and KS Bae. 2002. Diversity of yeasts associated with natural environments in korea. J. Microbiol. Biotechnol. 40:55-62

원문보기 상세보기
Lanfranco L, L Garnero, M Delpero and P Bonfante. 1995. Chitin synthase homo logs in three ectomycorrhizal truffles. FEMS Microbiol. Lett. 134:109-114

상세보기
Mehmann B, I Brunner and GH Braus. 1994. Nucleotide sequence variation of chitin synthase genes among ectomycorrhizal fungi and its potential use in taxonomy. Appl. Environ. Microbiol. 60:3105-3111

상세보기
Mellado E, A Autauvre-Brown, CA Specht, PW Robbins and DW Holden. 1995. A multigene family related to chitin synthase genes of yeast in the opportunistic pathogen Aspergillus fumigatus. Mol. Gen. Genet. 246:353-359

상세보기
Nam JS, DH Lee, HY Park and KS Bae. 1997. Cloning and phylogenetic analysis of chitin synthase gene from entomopathogenic fungus, Beauveria brongniartii. J. Microbiol. 35:222-227

원문보기 상세보기
Nam JS, DH Lee, KH Lee, HM Park and KS Bae. 1998. Cloning and phylogenetic analysis of chitin synthase genes from the insect pathogenic fungus, Metarhizium anisopliae var. anisopliae. FEMS Microbiol. Lett. 159:77-84

상세보기
Namgung J, BC Park, DH Lee, KS Bae and HM Park. 1996. Cloning and characterization of chitin synthase gene fragments from Penicillium chrysogenum. FEMS Microbiol. Lett. 145:71-76

상세보기
Peng M, SM Karuppayil, L Mendoza, TA Levins and PJ Szaniszlo. 1995. Use of the polymerase chain reaction to identify coding sequences for chitin synthase isozymes in Phialophora verrucosa. Curr, Genet. 27:517-523

상세보기
Saitou N and M Nei. 1987. The neighbor-joining method: A new method for reconstruction of phylogenetic trees. Mol. Biol. Evol. 4:406-425

상세보기
Sambrook J, EF Fritsch and T Maniatis. 1989. Molecular cloning: a laboratory Manual (2nd. ed.). Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
Shaw JA. PC Mol. B Bowers. SJ Silverman. MH Valdivieso. A. Duran and E. Cabib. 1991. The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle. J. Cell. Biol. 114:111-123

상세보기
Thompson JD, TJ Gibson, F Plewniak, F Jeanmougin and DG Higgins. 1997. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl. Acids. Res. 24:4876-4882
Thomsen L and A Beauvais. 1995. Cloning of two chitin synthase gene fragments from a protoplastic entomophthorale, FEMS Microbiol. Lett. 129:115-120

상세보기
Valdivieso MH, PC Mol, JA Shaw, E Cabib and A Duran. 1991. CALl, a gene required for activity of chitin synthase 3 in Saccharomyces cerevisiae. J. Cell BioI. 114:101-109

상세보기
Valerio E, M Gadanho and JP Sampaio. 2002. Sporobolomyces odoratus sp. nov., a new species in the Sporidiobolus ruineniae clade. FEMS Yeast Res. 2:9-16
Wang QM and FY Bai 2004. Four new yeast species of the genus Sporobolomyces from plant leaves. FEMS Yeast Res. 4:579-586

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[국내논문] Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea 원문보기

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[국내논문] Characterization and Phylogenetic Analysis of Chitin Synthase Genes from the Genera Sporobolomyces and Bensingtonia subrorea 원문보기

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