Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabba...
Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.
Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Th 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pfl, r 9-10, Rhcql-2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r9-10, Stock and Rh-cql-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pfl isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.
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문제 정의
rapa and attempted to correlate these differences with symptoms on indicator plants and serological and electrophoretic mobility. In this study, the representative eight isolates of TuMV selected from different areas and crops in Korea were correlatedwith their relationships based on the pathogenicity and phylogenetic analysis of CP genes. Additionally, we investigated and classified these eight isolates of TuMV using the selective dififerential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines.
제안 방법
, 1995), virus particle assembly (Sit et al, 1995) and cell-to- cell movement (Schmitz and Rao, 1998). Considering the various functions of the CP, this study adopted CP as a representative gene for investigation on evolutionary relationship between classical classification and molecular phylogenetic analysis.
Strain specificity is an important factor to be considered in developing resistant cultivars to TuMV since their performance will depend upon the presence and distribution of these strains in a given locality. The purposes of this diflferentiation were to determine the presence and prevalence of TuMV strains infecting in Korea, and to make those strains available for the development of stable yield and disease-resistant cruciferous varieties in the breeding program. Prowidenti (1981) classified TuMV isolates on the basis of differential host reactions to a select group of B.
frutescens var. japonica, P. floridana, B. campestris Sambo, Palweolje, Wonyae31, SSD63, and AVRDC, R. sativusVX 032528, LT 032538, and LT 032554 out of indicator host species, forty-eight inbred lines and two hybrids of Korean Chinese cabbage and fifty inbred lines of Korean radish were selected by the virulence differentiation to the representative six isolates of TuMVEight isolates including to the representative six isolates were used to divide the group by check the virulence differentiation. Three isolates, such as ca-ad7, cj-ca2-l and tu-1 belong to a group (Group I), whereas rg-pfl, r9-10, rh- cql-2, stock and mustard isolates also showed almost similar pattern of the virulence differentiation (Group II).
이론/모형
0. Fifty Chinese cabbage and Korean radish inbred lines and two Chinese cabbage hybrids, Tropical Delight and Crusader, were used for TuMV strain classification (Tables 3, 4 and 5). Three plants of each line and cultivar were inoculated in 3 replications.
The identity matrixes for each nucleotide and amino acid sequence were calculated by the program of BioEdit (Hall, 1999). TuMV sequences obtained were phylogenetically compared to those of the other TuMV (GenBank and EMBL) using the multiple sequence alignment application of DNAMAN version 4.0 (Lymon Biosoft, Quebec, Canada). Full optimal sequence alignments and neighborjoining method options of Saitou and Nei (1987) with 1000 bootstrap (Felstein, 1985) replications (Altschul et al.
성능/효과
pekinensis proposed by Prowidenti (1980) included commercial cultivars and Fl hybrids which were sometimes found to be genetically impure responses with respecting TuMV infection, and which show clear symptoms only in the cool season, making strain detection quite difficult (AVRDC, 1986). Therefore, five Chinese cabbage inbred lines (Palweolje, Sambo, Wonyae 20031, AVRDC, and SSD63), 3 Korean radish lines (LT 032528, LT 032538, and LT 032554) and 2 indicator host species (P. frutescens var. japonica and P. floridand) were used for more specific strain differentiation. This differential series has proved very useful in this context, and represents an important component for future integrated control strategies where resistance genes will play a major role in TuMV control.
후속연구
Additionally, we investigated and classified these eight isolates of TuMV using the selective dififerential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines. The information generated from this study is hoped to be of value inbreeding of TuMV-resistant varieties of cruciferous vegetables.
참고문헌 (26)
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