[논문]RAPD를 이용한 자생 Iris속 식물의 유전적 유연관계 분석

안영희

doi:10.5322/jes.2005.14.3.265

RAPD를 이용한 자생 Iris속 식물의 유전적 유연관계 분석
Analysis of Genetic Relationship of Native Iris species Plants using RAPD 원문보기

한국환경과학회지 = Journal of the environmental sciences, v.14 no.3, 2005년, pp.265 - 269

안영희 (Department of Applied Plant Science, Chung-Ang University)

Abstract ▼ AI-Helper

This study was carried out to provide the basic data for an identifying system for Iris species distributed in Korean market from complete analysing of genetic relationship between three native Iris species and one cultivar bred from the native Iris plant. RAPD analysis of genetic relationship among 4 Irises was possible. According to the RAPD analysis, they were divided into two groups. Among 4 Irises used in this study, Iris laevigata 'Veriegata', Iris laevigata and Iris setosa were classified into the same group since they had many similarities even though the habitat of Iris laevigata in Korean peninsular is restricted mainly in the south and Iris setosa is naturally inhabited in the northern part of Kangwondo. The value for the dissimilarity index of Iris laevigata and Iris laevigata 'Veriegata' was 6.757. The value for the dissimilarity index of Iris laevigata and Iris dichotoma was 95.000, so that they were genetically the farthest among them since the genetic relationship between two species are separated far if the value of the dissimilarity index is close to 100.

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가설 설정

0나!' study was carried out from August 2002 to June 2004. Four Iris plants used in this study were listed in Table 1. The samples of Iris setosa and Iris laevigata were collected in Mt. Odae and Mt. Chiri accordingly. Iris dichotoma and Iris laevigata 'Veriegata' were collected at Pyungkang Botanical Garden located in Pochungun, Kyungkido.

제안 방법

Iris laevigata 'Veriegata' is a cultivar developed for a horticultural purpose and used internationally for greening in damp area. In this study, therefore, analysis of genetic relationship among 4 Irises that have known distributed area, character and breeding process was conducted by RAPD. This study was carried out to provide the basic data for an identifying system for Iris species distributed in Korean market from complete analysing of genetic relationship between three native /ris species and one cultivar bred from the native Iris

이론/모형

The binary matrix was transformed into a similarity matrix according to the Nei and Li's method7) using Treecon8). From this matrix, phenogram was obtained by cluster analysis, UPGMA method (Unweighted pair-group method with arithmatic average), and a bootstrap analysis', was performed on it as the percent of 100 replication.
Based on the bands recorded as present or absent, a binary matrix was obtained. The binary matrix was transformed into a similarity matrix according to the Nei and Li's method7) using Treecon8). From this matrix, phenogram was obtained by cluster analysis, UPGMA method (Unweighted pair-group method with arithmatic average), and a bootstrap analysis', was performed on it as the percent of 100 replication.

성능/효과

blooming period and flower colors were very similar each other except leaves1 color and the white striped patterns on their leaves. Among 4 Irises used in this study, Iris laevigata 'Veriegata', Iris laevigata and Iris setosa were classified into the same group since they had many similarities even though the habitat of Iris laevigata in Korean peninsular is restricted mainly in the south and Iris setosa is naturally inhabited in the northern part of Kangwondo(Fig. 2). However, the morphological characteristics of flowers of Iris laevigata and Ms setosa were similar in appearance.
According to the RAPD analysis, they were divided into two groups. The result showed that there was not different in the genetic variation between Iris laevigata 'Veriegata; a horticultural cultivar developed for the white striped patterns on their leaves as an ornamental value from Iris laevigata, and its original species, Iris laevigata. From comparing to these two plants during cultivating, it was easy to know that plant's height,

후속연구

However, they become to lose their value as horticultural cultivars because nursery plants propagated by seed have undefined genetic cheacters from the natural crossing. For the native and cultivated Irises in Korea, therefore, an accurate survey should be done promptly in order to classify how many cultivars are grown at present and a precise identification method based on their characters should be established as well.

참고문헌 (10)

Lee, T. B., 1982, Illustrated flora of Korea. Hyangmoonsa publishing Co., Seoul, 638pp
Hotta, M., 1989, Useful plants of the world, Heibonsh a LTD., Tokyo, 525-527pp
Rani, V. A., K. Parida and S. N. Raina, 1995, Randomly amplified polymorphic DNA(RAPD) markers for genetic analysis in micropropagated plants Populus deltoides, Plant Cell Reports, 14, 459-462

상세보기
Ahn, Y. H., D. S. Park and K. Y. Chung, 2003, Analysis of genetic relationship among native Taraxacum and naturalized Taraxacum species using RAPD, The Korean Journal of Environment and Ecology, 17, 169-176

원문보기 상세보기
Benner, M. S., M. D. Braunstein and M. U. Weisberg, 1995, Detection of DNA polymorphisms within the genus Cattleya (Orchidaceae), Plant Mor. BioI. Rep., 13, 147-155

상세보기
Chalmer, K. J., R. Wauugh, J. K. Sprent, A. J. Simons and W. Powell, 1992, Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers, Heredity, 69, 465-472

상세보기
Nei, M. and W. H. Li, 1979, Mathematical model for studying genetic variation in terms of restriction endonucleases, Proc. Natl. Acad. Sci., 76, 5269-5273
Sneath, P. H. A. and R. R. Sokal, 1973, Numerical Taxonomy, W. H. Freeman, 341-347pp
Van De Peer, Y. and R. De Watcher, 1993, Treecon: A software package for the construction and drawing of evolutionary trees, Comput. Applic. Biosci., 9, 177-182
Felsenstein, J., 1985, Confidence limits on phylogenies: an approach using the bootstrap, Evolution, 39, 738-791

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RAPD를 이용한 자생 Iris속 식물의 유전적 유연관계 분석
Analysis of Genetic Relationship of Native Iris species Plants using RAPD 원문보기

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RAPD를 이용한 자생 Iris속 식물의 유전적 유연관계 분석 Analysis of Genetic Relationship of Native Iris species Plants using RAPD 원문보기

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RAPD를 이용한 자생 Iris속 식물의 유전적 유연관계 분석
Analysis of Genetic Relationship of Native Iris species Plants using RAPD 원문보기

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