[논문]재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성

금은주; 박상조; 이봉호; 김종식; 손건호; 손호용

doi:10.5352/jls.2006.16.4.647

재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성
Antifungal Activity of Phenanthrene Derivatives from Aerial Bulbils of Dioscorea batatas Decne 원문보기

생명과학회지 = Journal of life science, v.16 no.4 = no.77, 2006년, pp.647 - 652

금은주 (안동대학교 식품영양학과) , 박상조 (안동대학교 생명과학과) , 이봉호 (안동대학교 생명과학과) , 김종식 (경북농업기술원 생물자원연구소) , 손건호 (안동대학교 식품영양학과) , 손호용 (안동대학교 식품영양학과)

초록
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마속 식물들은 오래 전부터 다양한 민간처방에 사용되어 왔으며, 재배마의 근경은 다양한 생리활성물질들이 보고되어, 건강식품으로 인식되고 있다. 재배마의 주아는 매년 2,000톤 이상 생산되고 있으나, 다음해의 종자로 일부 이용될 뿐 거의 폐기되고 있으며, 생리활성에 대한 보고도 거의 없는 실정이다. 본 연구에서는 마의 주아로부터 3종의 phenanthrene 및 2종의 phenanthraquinone를 분리하였으며, 이들의 구조를 규명하였다. 이들 중 6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone은 처음으로 분리 되었다. 분리된 5종을 대상으로 항균활성을 평가한 결과, 6,7-dihydroxy-2,4-dimethoxyphenanthrene은 Candida albicans에 대해 $25\;{\mu}g/ml$의 MIC와 MFC를 나타내어, 강력한 항균활성으로 실제적 사용 가능성을 나타내었다. 본 연구결과는 phenanthrene 링 구조의 -OH 및 $-OCH_3$ 수식이 항균활성 및 항균 스펙트럼 결정의 중요한 인자임을 제시하였다.

Abstract ▼ AI-Helper

Plants of the genus Dioscorea have long been used as oriental folk medicine, and Dioscorea batatas Decne has been cultivated for healthy food in Korea. Although the bulbils were produced 2,000 ton annually, there are few reports for bioactive compounds in bulbils. In this study, three phenanthrenes and two phenanthraquinones were isolated from the aerial bulbils of D. batatas Decne, and their structures were elucidated. Among them, compound 2 (6-hydroxy-2,7-dimethoxy-1,4-phenanthraquinone) has not been reported previously. Evaluation of antimicrobial activities based on disk-diffusion assay, MIC and MFC showed the compound 12 (6,7-dihydroxy-2,4-dimethoxyphenanthrene) has strong antimicrobial activity with $25\;{\mu}g/ml$ of MIC and MFC against Candida albicans. Our results suggested that compound 12 has a potent antifungal activity, and the antimicrobial activity and its spectrum are modulated by hydroxylation and methoxylation of phenanthrene ring moiety of the compound.

주제어

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가설 설정

1 CA: Candida albicans, BC: Botrgtis cinerea, SC: Saccharomyces cerevisiae, PV: Proteus vulgaris, ST: Salmonella typhimuriumf EC: Escherichia coli 0157, SE: Staphylococcus epidermidis, SM: Streptococcus mutans, SA: Staphylococcus aureus. 2 : No inhibition.
3 : No inhibition.

제안 방법

In this study, we isolated different phenanthrenes and phenanthraquinones from the aerial bulbils of D. batatas Decnez and evaluated antimicrobial activities of isolated phenanthrene derivatives. Our results suggest that 6, 7-di- hydroxy-2/4-dimefhoxyphenanthrene possess a potential as an antifungal agent, and the antimicrobial activity and its spectrum may be modulated by hydroxylation and me- thoxylation of phenanthrene ring moiety of the compound.

대상 데이터

batatas Decne) were har vested from farm of the Gyeongbuk Agricultural Technology Administration/ Kyungpook, Korea in November, 2002. A voucher specimen (A-0360) was deposited at the Department of Food and Nutrition, Andong National University, Korea. The aerial bulbils of yam were chopped, dried at 60 ℃, ground to powder, and stored at 4℃ before use.
Bacterial strains used were Proteus vulgaris KCTC 2433, Salmonella typhimurium KCTC 1926, Escherichia coll 0157 ATCC 43895, Staphylococcus epidermidis ATCC 12228/ Streptococcus mutans JC-Z and Staphylococcus aureus KCTC 1916. The antibacterial activity was evaluated by disk-dif fusion assay as determined by growth inhibition zone [18, 20].

후속연구

These results indicate that compound 12 has a prominent fungicidal activity, and suggest that 6, 7~dihy- droxy-24-dimethoxyphenanthrene in the bulbils of yam is responsible for defense mechanism against microbial infection. Further researches on the mode of action of 6/7-dihydroxy-2/4-dimethoxyphenanthrene, and modification of the structure for activity increase are necessary.

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