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Differential Transformation of Ginsenosides from Panax ginseng by Lactic Acid Bacteria 원문보기

Journal of microbiology and biotechnology, v.16 no.10, 2006년, pp.1629 - 1633  

Chi, Hyun (Department of Food and Nutrition, Seoul National University) ,  Lee, Bo-Hyun (Department of Food and Nutrition, Seoul National University) ,  You, Hyun-Ju (Department of Food and Nutrition, Seoul National University) ,  Park, Myung-Soo (Department of Hotel Culinary Art, Anyang Technical College) ,  Ji, Geun-Eog (Department of Food and Nutrition, Seoul National University)

Abstract ▼ AI-Helper 아이콘AI-Helper

Ginsenosides have been regarded as the principal components responsible for the pharmacological and biological activities of ginseng. The transformation of ginsenosides with live lactic acid bacteria transformed ginsenosides Rb2 and Rc into Rd, but the reactions were slow. When the crude enzymes obt...

주제어

#Ginsenosides   #transformation   #lactic acid bacteria  

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  • ). HPLC analyses were conducted using an HPLC pump (model 526, Alltech Associates, Deerfield, IL, U.S.A.), a normal­ phase column (5 pm, 250 mm><4.6 mm; Prevail Carbohydrate ES, Alltech Associates), an evaporative light scattering detector (ELSD; model 800, Alltech Associates), and Allchrom Plus software. Elution was performed at a flow rate of 0.
  • In this study, we focused on the production of specific deglycosylated ginsenosides by using ginseng extract as substrate, and enzyme preparations obtained from selected lactic acid bacteria, and characterized the microbial transformation of the ginsenosides involved.

대상 데이터

  • Four Bifidobacterium strains previo니sly reported [18, 14] [Bifidobacterium sp. Int57 (Int57), Bifidobacterium bifidum BGN4 (BGN4), Bifidobacterium sp. SJ32 (SJ32), and Bifidobacterium sp. SH5 (SH5)] and Leuconostoc paramesenteroides PR [6] were used. Lactobacillus delbrueckii ssp.
  • ); Rgl was purchased from Wako Pure Chemical Industries (Osaka, Japan); Rd and Rg3 were purchased from BTGin (Chungnam, Korea); Rf was purchased from Molecular Neurophysiology Biomedical Research Center, Korea Institute of Science and Technology (Seoul, Korea); and compound K was a kind gift from the Department of Pharmaceutical Science, Kyung Hee University (Seoul, Korea). Six-year-old root hair of Panax ginseng was purchased from Kyoung-Dong Market in Seoul, Korea.
  • Lactobacillus delbrueckii ssp. delbrueckii KCTC 1047 (L. delbrueckii) was purchased from the Korean Collection for Type Cultures (Daeje이i, Korea). The bacterial strains were incubated in MRS medium (Hardy, Santa Maria, CA, U.
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참고문헌 (24)

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    상세보기 crossref

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    상세보기 crossref

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  13. Karikura, M., H. Kato, F. Shimada, and S. Yano. 1992. Studies on the enzyme immunoassay of bioactive constituents contained in oriental medicinal drugs. VI. Enzyme immunoassay of ginsenoside Rb1 from Panax ginseng. Chem. Pharm. Bull. 40: 314-317 

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