[논문]Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sohn, Sea-Hwan; Cho, Eun-Jung; Jang, In-Surk

Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1 원문보기

Reproductive & developmental biology = 한국동물번식학회지, v.30 no.4, 2006년, pp.263 - 270

Sohn, Sea-Hwan (Department of Animal Science and Biotechnology, Jinju National University) , Cho, Eun-Jung (Department of Animal Science and Biotechnology, Jinju National University) , Jang, In-Surk (Department of Animal Science and Biotechnology, Jinju National University)

Abstract ▼ AI-Helper

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

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제안 방법

Therefore, in this study, we tried to establish the origin of each chromosome by means of G~band markers and the distribution of telomeric DNA on chromosomes. According to the karyotype of CHOK1 cells, we provided the nomenclature and designation of these chromosomes as described in Table 1.
To evaluate the cytogenetic characteristics of CHOK1 cells, the frequency and type of chromosome aberrations at metaphase were analyzed. Of the 822 metaphase spreads, 68 (8.
To observe the distribution of telomeres on CHOK1 chromosomes, FISH using a human telomeric DNA probe was performed. Fig.

대상 데이터

Chinese hamster ovarian cells, CHQK1 cell line (Cell No. KCLB 10061), were obtained from the Korean Cell Line Bank (Daejeon, Korea) to investigate their cytogenetic characteristics.

이론/모형

G-banding was performed using air dried slides according to the method of Wang and Federoff (1974). Briefly, the obtained slides were immersed in 0.

성능/효과

On the other hand, Deaven and Petersen (1973) conducted G- and C- banding as well as autoradiographic analysis to exhibit the exact karyotype of CHO cells. As a result, they revealed that the chromosome modal number of CHO cells was 2n=21; 8 of the 21 chromosomes including 1, 2/ X, 6, 8Z 9, 10 and 11 turned out to be identical compared with those of the Chinese hamster, but the remaining rearranged 13 chromosomes were classified as Z group. Park and Lee (1984) found that the chromosome modal number of CHO cells was 2n=20; 7 of the 20 chromosomes including two pairs of hom이 ogous chromosomes 1 and 6, and one of chromosomes 2f 7 and 10 were identical with the Chinese hamster chromosomes, whereas the remnant 13 were shown to be deleted or added chromosomes.
They also found two deleted chromosomes such as chromosomes 3p- and 6q-z five translocated chromosomes designated as Z set including 72, ZA, Z7, Z8z and Z13z and four newly rearranged chromosomes as R set including Rl, R2Z R3, and R4. Taken together, it could be concluded that the modal number of chromosomes derived from CHO cells was 2n=19~21; at least 7 chromosomes were identical with the Qiinese hamster chromosomes but the remaining chromosomes resulted in morphological alterations consisting of translocated, deleted, inversed, and/or rearranged chromosomes. In this study, the karyotype of CHOK1 cells was analyzed as 2n=20.
1. The results showed that the karyotype of CHOK1 cells was 2n=20 which consisted of some normal Qiinese hamster chromosomes and newly rearranged chromosomes. Eight chromosomes induding two pairs of homologous chromosomes 1 and 5, and one of chromosomes 2f 6, 8 and 9 appeared to be identical with Chinese hamster chromosomes, whereas the remaining twelve chromosomes were shown to be translocated, deleted, inversed and/or rearranged chromosomes from the Chinese hamster chromosomes.

후속연구

In particular, CHO cells are the most frequently applied and well accepted to evaluate effectiveness and genotoxicity in response to pharmacological agents. Thus, the results of this study regarding the frequency and type of naturally occurred chromosome aberrations in CHOK1 cell lines would be beneficial for the evaluation and the assessment of genotoxic substances.

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Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1 원문보기

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Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1 원문보기

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