[논문]Antioxidant Activity of Extracts from Dangyuja (Citrus grandis Osbeck) Fruits Produced in Jeju Island

Lim, Hee-Kyoung; Yoo, Eun-Sook; Moon, Jeong-Yong; Jeon, You-Jin; Cho, So-Mi K.

[국내논문] Antioxidant Activity of Extracts from Dangyuja (Citrus grandis Osbeck) Fruits Produced in Jeju Island 원문보기

Food science and biotechnology, v.15 no.2 = no.68, 2006년, pp.312 - 316

Lim, Hee-Kyoung (Department of Medicine, Cheju National University) , Yoo, Eun-Sook (Department of Medicine, Cheju National University) , Moon, Jeong-Yong (Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University) , Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University) , Cho, So-Mi K. (Facuity of Biotechnology, College of Applied Life Sciences, Cheju National University)

Abstract ▼ AI-Helper

Crude extract of young dangyuja (Citrus grandis Osbeck) fruit was investigated for its antioxidant activity as measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Among the samples, including 4 Citrus species and various solvent-extracted-fractions of young dangyuja fruit, the water-extracted fraction (WF) and butanol-extracted fraction (BF) showed the greatest DPPH free radical scavenging activity. WF and BF were further examined for their antioxidant activities by three different in vitro assays. The cell viability tests using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay showed that both fractions significantly reduced $H_2O_2$-induced cytotoxicity in HepG2 cells dose-dependently. Generation of the reactive oxygen species (ROS) was also reduced in cells pretreated with both fractions. In addition, BF showed a higher level of lipid peroxidation inhibitory capacity than WF in $H_2O_2$-treated HepG2 cells. Taken together, these results indicate that young dangyuja fruit can be used as an easily accessible source of natural antioxidants.

Keyword

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

sinensis, mature dangytga, and young dangyuja. Young dangyuja whole fruit extracts and residues were prepared for examination using successive solvents of varying polarity and by partitioning the methanol fraction with hexane, chloroform, ethyl acetate, and n-butanol. Furthermore, the effect of water- and butanol-extracted fractions (WF and BF, respectively) of young dangyuja fruit on antioxidant defense at the cellular level was investigated by a human hepatoma HepG2 cell line, for the first time.

대상 데이터

Dangyuja fruits were obtained from the National Institute of Subtropical Agriculture Jeju Province, Korea. Young fruits were collected on June 28, 2005 from dangyuja trees about 30 days after flowering and the mature fruits were collected in January 2005.
The human hepatoma HepG2 (KCLB No. 58065) cells were purchased from the Korea Cell Line Bank (Seoul, Korea). They were grown in Dulbecco's Modified Eble's Medium (DMEM) supplemented with 10%(v/v) heat-inactivated fetal bovine serum (FBS), 100 units/mL of penicillin, and 100 |ig/mL of streptomycin.

데이터처리

Experimental data were analyzed by Studenfs /-test and expressed as mean ± SD. A probability value oft?<0.

이론/모형

The free radical scavenging activity of the fruit extracts was measured by the DPPH radical assay described by Blois (21). Total extracts of citrus fruit at a final concentration of 0.
The cell viability was estimated by the conventional 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) reduction assay, which is based on cleavage of a tetrazolium salt by mitochondria dehydrogenases in viable cells (22). HepG2 cells were cultured at a density of IxlO₅ cells/mL on 96-well microplates for 16 hr, washed twice using PBS, and pretreated with different concentrations of dangyuja fruit extracts.
Lipid peroxidation was assayed by measuring malondialdehyde (MDA) according to the method of Ohkawa et al. (24). Lipid peroxides react with thiobarbituric acid (TBA) and develop a pink color due to the formation of TBARS (TBA reactive substances).

참고문헌 (25)

Kehrer JP. Free radicals as mediators of tissue injury and disease. Crit. Rev. Toxicol. 23: 21-48 (1993)

상세보기
Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Crit. Rev. Food Sci. Nutr. 44: 275-295 (2004)

상세보기
Ames BN, Shigenaga MK, Hagen TM. Oxidants, antioxidants, and the degenerative diseases of aging. P. Natl. Acad. Sci. USA 90: 7915-7922 (1993)
Fiers W, Beyaert R, Declercq W, Vandenabeele P. More than one way to die: apoptosis and necrosis and reactive oxygen damage. Oncogene 18: 7719-7730 (1999)

상세보기
Deng G, Su JH, Ivins KJ, Houten BY, Cotman CW. Bcl-2 facilitates recovery from DNA damage after oxidative stress. Exp. Neurol. 159: 309-318 (1999)

상세보기
Satoh I, Sakai N, Enokido Y, Uchiyama Y, Hatanaka H. Free radical independent protection by nerve growth factor and Bcl-2 of PC12 cells from hydrogen peroxide triggered-apoptosis. J. Biochem. 120: 540-546 (1996)

상세보기
Oliva MR, Iradi A, Garrido F, Ramos M, Oltra AM, Muniz P, Saez GT. Oxidative stress induces the expression of the major histocompatibility complex in murine tumor cells. Free Radical Res. 35: 119-128 (2001)

상세보기
Ng TB, Liu F, Wang ZT. Antioxidative activity of natural products from plants. Life Sci. 66: 709-723 (2000)

상세보기
Park YK, Lee WY, Park SY, Ahn JK, Han MS. Antioxidant activity and total phenolic content of Callistemon citrinus extracts. Food Sci. Biotechnol. 14: 212-215 (2005)

원문보기 상세보기
Choi YM, Ku JB, Chang HB, Lee JS. Antioxidant activities and total phenolics of ethanol extracts from several edible mushrooms produced in Korea. Food Sci. Biotechnol. 14: 700-703 (2005)

원문보기 상세보기
Heo SJ, Park PJ, Park EJ, Cho SM, Kim SK, Jeon, YJ. Antioxidative effect of proteolytic hydrolysates from Ecldonia cava on radical scavenging using ESR and HzOz-induced DNA damage. Food Sci. Biotechnol. 14: 614-620 (2005)

원문보기 상세보기
Ross JA, Kasum CM. Dietary flavonoids; bioavailability, metabolic effects, and safety. Annu. Rev. Nutr. 22: 19-34 (2002)

상세보기
Huang HY, Alberg AJ, Norkus EP, Hoffinan SC, Comstock GW, Helzlsouer KJ. Prospective study of antioxidant micronutrients in the blood and the risk of developing prostate cancer. Am. J. Epidemiol. 157: 335-344 (2003)

상세보기
Knekt P, Jarvinen R, Seppanen R, Hellovaara M, Teppo L, Pukkala E, Aromaa A. Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. Am. J. Epidemiol. 146: 223-230 (1997)

상세보기
Miller NJ, Rice-Evans CA. The relative contributions of ascorbic acid and phenolic antioxidants to the total antioxidant activity of orange and apple fruit juices and blackcurrant drink. Food Chem. 60: 331-337 (1997)

상세보기
Rapisarda P, Tomaino A, Lo Cascio R, Bonina F, De Pasquale A, Saija A. Antioxidant effectiveness as influenced by phenolic content of fresh orange juices. J. Agric. Food Chem. 47: 4718-4723 (1999)

상세보기
Roberts, QG, Gordon, MH. Determination of the total antioxidant activity of fruits and vegetables by a liposome assay. J. Agric. Food Chem. 51: 1486-1493. (2003)

상세보기
Mokbel MS, Hashinaga F. Evaluation of the antioxidant activity of extracts from butan (Citrus grandis Osbeck) fruit tissues. Food Chem. 94: 529-534 (2006)

상세보기
Sun, CD, Chen, KS, Chen, Y, Chen. QJ. Contents and antioxidant capacity of limonin and nomilin in different tissues of citrus fruit of four cultivars during fruit growth and maturation. Food Chem. 93: 599-605 (2005)

상세보기
Alia M, Ramos S, Mateos R, Bravo L, Goya L. Response of the antioxidant defense system to t-Butyl hydroperoxide and hydrogen peroxide in a human hepatoma cell line (HepG2). J. Biochem. Mol. Toxic. 19: 119-128 (2005)

상세보기
Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 181: 1199-1200 (1958)

상세보기
Hansen MB, Nielsen SE, Berg K. Re-examination and further development of a precise and rapid dye method for measuring cell growth! cell kill. J. Immunol. Method 119: 203-210 (1989)

상세보기
Wang H, Joseph JA. Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radical. Bio. Med. 27: 612-616 (1999)

상세보기
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95: 351-358 (1979)

상세보기
Heim KE, Tagliaferro AR, Bobilya, DJ. Flavonoid antioxidants: chemistry and structure-activity relationships. J. Nutr. Biochem. 13: 572-584 (2002)

상세보기

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증