$\require{mediawiki-texvc}$
  • 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
안녕하세요!
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

곡류 내 비타민 E 분석을 위한 추출방법의 비교

Comparison of Extraction Methods for the Determination of Vitamin E in Some Grains

초록

식품내의 비타민 E를 분석하는 방법은 여러 가지 방법이 있으나 최근에는 비타민 E의 8가지 동족체를 모두 분석할 수 있는 HPLC 방법 이 가장 많이 이용되고 있다. 비타민 E를 분석함에 있어 가장 중요한 과정은 식품으로부터 비타민 E를 추출하는 과정이며 검화방법, 직접용매추출법, Soxhlet 추출법 등이 이용되고 있다. 본 연구에서는 곡류 14종을 대상으로 세 가지 추출방법을 비교하였으며 직접용매추출법과 Soxhlet 추출법을 이용하였을 경우 검화방법보다 높은 분석치를 얻을 수 있었다. 곡류에서의 비타민 E의 함량을 측정한 결과를 a-TE로 나타내었으며 흑미에서 5.12 a-TE/100g으로 가장 높았고 기장에서 0.32 a-TE/100 g으로 가장 낮은 함량을 나타내었다.

Abstract

For the determination of vitamin I using high performance liquid chromatography (HPLC), the most critical and time consuming step is the Quantitative extraction of all vitamin E homologs from sample matrix. Three different extraction methods were compared to determine vitamin E in grains. Saponification used alkaline hydrolysis followed by solvent extraction. Direct solvent extraction included dispersing the samples in hot water, addition of isopropanol and $MgSO_4$, and extraction of the analytes with extracting solvent with Polytron homogenization. Using Soxhlet extraction, the samples were extracted in a Soxhlet apparatus with hexane containing butylated hydroxytoluene (BHT). Vitamin E content in 14 grains were analyzed and compared by three different extraction methods. Generally, the highest values were observed from direct solvent extraction and Soxhlet extraction followed by saponification. a-Tocopherol equivalent (a-TE) levels in grains ranged from 0.32 a-TE/100 g in prosomillet to 5.12 a-TE/100 g in black rice.

참고문헌 (15)

  1. Zielinski H, Kozlowska H, Lewczuk B. 2001. Bioactive compounds in the cereal grains before and after hydro-thermal processing. Innovat Food Sci Emerg Technol 2: 159-169 
  2. Hill MJ, Path FR. 1998. Cereals, dietary fiber and cancer. Nutr Res 18: 653-659 
  3. EU-Air Concerted Action CT 94 2185. 1998. Nettox Com-pilation of Consumption Data. Report No. 4. Published by Danish Veterinary and Food Administration, Denmark. p 20-21 
  4. Piironen V, Varo P, Syvaoja EL, Salminen K, Koivistoinen P. 1984. High performance liquid chromatographic deter-mination of tocopherols and tocotrienols and its application to diets and plasma of Finnish men. Internat J Vit Nutr 53: 35-40 
  5. Eitenmiller RR, Landen WO Jr. 1995. Vitamin. In Analyzing Food for Nutrition Labeling and Hazardous Contaminants. Jeon I, Ikins W, eds. Marcel Dekker, New York, USA. p 185-281 
  6. Lee J, Landen WO Jr, Phillips RD, Eitenmiller RR. 1998. Application of direct solvent extraction to the LC quanti-fication of vitamin E in peanuts, peanut butter and selected nuts. Peanut Sci 25: 213-218 
  7. Lee J, Ye L, Landen WO Jr, Eitenmiller RR. 2000. Optimization of an extraction procedure for the quantitation of vitamin E in tomato and broccoli using response surface methodology. J Food Comp Anal 13: 45-57 
  8. Lee J, Suknark K, Kluvitse Y, Phillips RD, Eitenmiller RR. 1999. Rapid, liquid chromatographic assay of vitamin E and retinyl palmitate in extruded weaning foods. J Food Sci 64: 968-972 
  9. Prior WA. 1995. Vitamin E Abstract. The Vitamin E Research and Information Service. LaGrange, IL, USA. p VII 
  10. Carpenter DL, Slover HT. 1973. Lipid composition of selected margarines. J Am Oil Chem Soc 50: 372-376 
  11. Weiser H, Vecchi M. 1981. Stereoisomers of alpha-to-copheryl acetate: Characterization of the samples by physico-chemical methods and determination of biological activities in the rat resorption-gestation test. Int J Vitam Nutr Res 51: 100-113 
  12. Qureshi AA, Qureshi N, Wright JJK, Shen S, Kramer G, Gabor A, Chong YH, DeWitt G, Ong ASH, Peterson D, Bradlow BA. 1991. Lowering of serum cholesterol in hypercholesterolemic humans by tocotrienols (palmvitee). Am J Clin Nutr 53: 1021S-1026S 
  13. Lang JK, Schillaci M, Irvin B. 1992. Vitamin E. In Modern Chromatographic Analysis of Vitamins. 2nd ed. Deleenheer AP, Lambert WE, Nelis HJ, eds. Marcel Dekker, New York, USA. p 153-195 
  14. Piironen V, Syvaoja EL, Varo P, Salminen K, Koivistoinen P. 1985. Tocopherols and tocotrienols in Finnish food: Meat and meat products. J Agric Food Chem 33: 1215-1218 
  15. Kim KA, Jeon ER. 1996. Physicochemical properties and hydration of rice on various polishing degrees. Korean J Food Sci Technol 28: 959-964 

이 논문을 인용한 문헌 (7)

  1. Choi, Young-Min ; Lim, Ho ; Woo, Sun-Hee ; Kim, Hong-Sik ; Jong, Seung-Keun ; Lee, Jun-Soo 2007. "Lutein Contents of Soybeans (Glycine max L.) Cultivated in Korea" 한국식품과학회지 = Korean journal of food science and technology, 39(5): 580~583 
  2. Seo, Sun-Jung ; Choi, Young-Min ; Lee, Sun-Mi ; Kim, Kee-Jong ; Son, Jong-Rok ; Lee, Jun-Soo 2007. "Determination of Selected Antioxidant Compounds in Specialty Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 36(4): 499~502 
  3. Seo, Sun-Jung ; Choi, Young-Min ; Lee, Seon-Mi ; Kong, Su-Hyun ; Lee, Jun-Soo 2008. "Antioxidant Activities and Antioxidant Compounds of Some Specialty Rices" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 37(2): 129~135 
  4. Kong, Suh-Yun ; Choi, Young-Min ; Lee, Seon-Mi ; Lee, Jun-Soo 2008. "Antioxidant Compounds and Antioxidant Activities of the Methanolic Extracts from Milling Fractions of Black Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 37(7): 815~819 
  5. Kong, Su-Hyun ; Choi, Young-Min ; Kim, Young-Wha ; Kim, Dae-Jung ; Lee, Jun-Soo 2009. "Antioxidant Activity and Antioxidant Components in Methanolic Extract from Geumjong Rice" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 38(6): 807~811 
  6. Cho, Young-Sook ; Kim, Yu-Na ; Kim, Su-Yeonk ; Kim, Jung-Bong ; Kim, Heon-Woong ; Kim, Se-Na ; Kim, So-Young ; Park, Hong-Ju ; Kim, Jae-Hyun 2011. "Changes in Fatty Acid Composition of Grain after Milling" 한국환경농학회지 = Korean journal of environmental agriculture, 30(4): 409~413 
  7. Lee, Jeom-Sig ; Park, No-Bong ; Lee, Jong-Hee ; Cho, Jun-Hyeon ; Won, Yong-Jae ; Park, Hyang-Mee ; Chun, A-Reum ; Jang, Jae-Ki ; Gwak, Do-Yeon ; Song, Yu-Cheon ; Hwa, Woon-Gu ; Yi, Gi-Hwan ; Yeo, Un-Sang 2012. "Optimum Milling Degree for Improving Sensory Quality of Cooked Rice" Korean journal of crop science = 韓國作物學會誌, 57(4): 359~364 

원문보기

원문 PDF 다운로드

  • ScienceON :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

DOI 인용 스타일