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논문 상세정보

사료 첨가용 생균제를 위한 Probiotics 유산간균의 분리 및 동정

Isolation and Identification of Probiotic Lactobacillus Isolates for Calf Meal Supplements

초록

한우와 홀스타인의 분변으로부터 MRS 배지와 LAPT 배지를 이용하여 무작위 선발법으로 54균주의 유산균을 1차로 분리하였다. 1차로 분리된 54균주에 대해 내담즙성이 우수한 10균주를 분리한 다음 내산성을 조사한 결과 인공위액 pH 2.5에서 LS1, LS15 및 LL6 균주가 각각 66.5%, 82.6% 및 80.7%의 생존율을 나타내었다. Sal. typhimurium, Sta. aureus 및 Cl. perfringens의 병원균에 대해 가장 큰 항균력을 보인 균주는 LL6와 LL7이었다. API CHL kit로 동정한 결과 LS1, LS2 및 LMI 균주는 모두 L. fermentum, LL6와 LL7 균주는 L. acidophilus, LS3 균주는 L. plantarum으로 각각 동정되었고, 나머지 4균주는 Lactobacillus spp. 로 동정되어 분리된 10균주 모두 안전성 있는 유산간균임을 확인하였다. 10종류의 항생제에 대한 내성을 조사한 결과 ampicillin, amoxicillin과 erythromycin에 대해서는 감수성이 있으나 colistin과 ciprofloxacin에 대해 모두 내성을 나타내었다. LB1, LL6 및 LL7 균주는 gentamicin과 neomycin에 대해 내성을 보여 주었다. 분리 동정된 균주 중에 내산성, 내담즙성 및 병원성 균에 대한 특성을 기준으로 판단할 경우 probiotic 유산균으로 사용 가능성이 높은 것은 LL6인 L. acidophilus로 나타났다.

Abstract

Fifty four acid-resistant and bile-resistant isolates of lactic acid bacteria were initially isolated from the faces of Korea native cattle and Holstein using MRS agar and LAPT agar, and ten strains with superior activity against bile salt were finally selected LS1, LS15, and LL6 isolates showed survival of 66.5%, 82.6% and 80.7% against the simulated stomach liquid(pH 2.5), respectively. LL6 and LL7 isolates had the highest inhibitory activities against the pathogenic bacteria such as Salmonella typhimurium, Staphylococcus aureus, and Clostridium perfringens. By using API 50 CHL kit LS1, LS2 and LM1 isolates were identified as a L. fermentum. LL6 and LL7 isolates as a L. acidophilus, and LS3 isolate as a L. plantarum. The other four isolates belong to genus Lactobacillus. All the isolates tested were sensitive to some antibiotics such as ampicillin, amoxicillin and erythromycin, but resistant to colistin and ciprofloxacin. LB1, LL6 and LL7 isolates were resistant to gentamicin and neomycin. Especially, the LL6 isolate showed the highest resistance to both of the simulated stomach liquid and bile salt, in addition to the highest inhibitory activities against Sal. typhimurium, Staph. aureus and Cl. perfringens.

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참고문헌 (33)

  1. Curragh, H. J. and Collins, M. A. (1992) High levels of spontaneous drug resistance in Lactobacillus. J. Appl. Bacteriol. 73, 31-36 
  2. Fahy, V. A., Connaughton, D., Dresen, S. J., and Spicer, E. M. (1987) Preweaning colibacillosis. In: Manipulating pig production. APSA Committee(ed.), Austrian Pig Science Association Werribee. Victoria. Australia, pp. 176-188 
  3. Hardie, J. M. (1986) Genus Streptococcus, In: Bergey's manual of systematic bacteriology. Sneath P. H. A.(ed.), Williams & Wilkins, London, Vol. 2, pp. 1043-1047 
  4. Kim, Y. M. (1999) The current status and prospect of using probiotics lactic acid bacteria in domestic animals. Bioindustry News(Kor.) 12, 23-28 
  5. Lilly, D. M. and Stillwell, R H. (1965) Probiotics : Growth promoting factors produced by microorganism, Science 147, 747-748 
  6. Salminen, S., von Wright, A., Morelli, L., Marteau, P., Brassart, D., Vos de, W. M., Fonde'n, R., Saxelin, M., Collins, K., Mogensen, G., Birkeland, S. E., and Sandholm, T. M., (1998) Demonstration of safety of probiotics - a review. Inter. J. Food Microbiol. 44, pp. 93-106 
  7. Sedlacek, O. and Rucki, J. (1976) Incidence of inhibitory substance-residues in meat and organs of slaughter calves fed with milk feed mixtures. Vet. Med.(Praha) 21, 137-148 
  8. Simpson, K. L., Pettersson, B., and Priest, F. G. (2001) Characterization of lactobacilli from Scotch malt whisky distilleries and description of Lactobacillus jerintoshensis sp. nov., a new species isolated from malt whisky fermen­tations. Microbiol. 147, 1007-1016 
  9. Wallace, R. J. and Newbold, C. J. (1992) Probiotics for ruminant, In : Probiotics : The scientific basis. Fuller, R (ed.), Chapmann & Hall, London. pp. 317-353 
  10. Heyman, M. and Menard, S. (2002) Probiotic microor­ganism: how they affect intestinal pathophysiology. Cell. Mol. Life Sci. 59, 1151-1165 
  11. Saarela, M., Mogensen, G., Fonden, R., Matto, J., and Mattila-Sandholm, T. (2000) Probiotic bacteria: safety, functional and technological properties. J. Biotechnol. 84, 197-215 
  12. Lee, J. Y., Hwang, K. Y., Kim, H. S., Kim, K., and Sung, S. I. (2002) Isolation and identification of lactic acid bacteria inhibting gastro-intestinal pathogenic bacteria of domestic animal. Kor. J. Microbiol. Biotechnol. 30, 129­-134 
  13. Fons, M, Hege, T., Ladire, M, Raibaud, P., Ducluzeau, R., and Maguin, E. (1997) Isolation and characterization of a plasmid from Lactobacillus fermentum conferring erythro­mycin resistance. Plasmid 37, 199-203 
  14. Mustapha, A., Jiang, T., and Savaiano, D. A. (1997) Impro­vement of lactose digestion by humans following ingestion of unfermented acidophilus milk: influence of bile sensi­tivity, lactose transport, and acid tolerance of Lactobacillus acidophillus. J. Dairy Sci. 80, 1537-1545 
  15. Wu, J. F. (1987) The microbiologist's function in develo­ping action specific microorganisms. In: Biotechnology in the feed industry. Lyons, T. P.(ed.). Alletech, Inc. Nicholas ville, Kentucky. pp. 181 
  16. Gilliand, S. E. and Speck, M. L. (1977) Antagonistic action of Lactobacillus acidophillus toward intestinal and food­borne pathogens in associative cultures. J. Food Prot. 40, 820-823 
  17. Piva, G., Belladonna, S., Fusconi, G., and Sicbaldi, F. (1993) Effects of yeast on dairy cow performance, ruminal fermentation, blood components and manufacturing pro­perties. J. Dairy Sci. 76, 2717-2722 
  18. Sanders, M. E. (2000) Considerations for use of probiotic bacteria to modulate human health. J. Nutr. 130, 384S­-390S 
  19. Hammes, W. P. and Vogel R. F. (1995) The genus Lacto­bacillus In: The genera of lactic acid bacteria. The lactic acid bacteria. Wood, B. J. and Holzapfel, W. H. N. (eds), Blackie Academic & Professional, Glasgow, Scotland Vol. 2, pp. 19-54 
  20. Mundt, J. O. (1986) Enterococci, In: Bergey's Manual of Systematic Bacteriology. Sneath P. H. A(ed.), Williams & Wilkins, London. Vol. 2, pp. 1063-1065 
  21. Adams, M. R. and Marteau, P. (1995) On the safety of lactic acid bacteria from food. Inter. J. Food Microbiol. 27, 263-264 
  22. Kohker, E. M. and Bohl, E. M. (1964) Prophylaxis of diarrhea in newborn pigs. J. American Vet. Med. Association. 144, 1794-1797 
  23. Ahn, C., Thompson, D. C., Duncan, C. and Stiles, M. E. (1992) Mobilization and location of the genetic determinant of chloramphenicol resistance from Lactobacillus planta­rum ca TC2R. Plasmid 27,169-176 
  24. Dixon, B. (2000) Antibiotics as growth promoters : Risks and alternatives. ASM News. 66, 264-265 
  25. Fuller, R. (1989) Probiotics in man and animal. J. Appl. Bacteriol. 66, 365-378 
  26. Tannock, G. W. (1983) Effect of dietary and environmental stress on the gastrointestinal micobiota, In: Human Intes­tinal. Microflora in Health and Disease, Hentges, D. J. (ed.), Academic Press, New York. pp. 517-539 
  27. Park, J. C., Kim, I. S., Kwon, S. K., Noh, J. M., Lee. S. M., Park, J. P., Lee, W. K., and Ryu, S. R. (2000) Pre­valence of anti-biotic-resistant strains among bacteria isolated from bovine mastitis, swine diarrhea, and swine pneumonia. Kor. J. Appl. Microbiol. Biotechnol. 28, 189­-194 
  28. Havenaar, R., Brink, B. T., and Veid, J. H. J. I. (1992) Selection of strains for probiotic use. In: Probiotics : The scientific basis, Fuller R(ed.) Chapmann & Hall, London. pp. 209-224 
  29. Bae, H. C., Paik S. H., and Nam, M. S. (2004) Fermen­tation properties of rice added yogurt made with various lactic acid bacteria. J. Anim. Sci. & Technol. (Kor.) 46, 677-686 
  30. Rose, A. H. (1980) Recent research on industrially impor­tant strains of Saccharomyces cerevisiae. In: Biology and Activitics of Yeast. Skinner, F. A, Passmore, S. M., and Daven, R. R(eds.), The Society for Applied Bacteriology Symposium Series. Academic Press, London, Vol. 9, pp. 103 
  31. Parker, R. B. (1974) The other half of the antibiotics story. Anim. Nutr. Health 29, 4-8 
  32. Tannock, G. W., Luchansky, J. B., Miller, L., Connell, H., Thode-Andersen, S., Mercer, A. A., and Klaenhammer, T. R. (1994) Molecular characterization of a plasmid-borne (pGT633) erythromycin resistance determinant (ermGT) from Lactobacillus reuteri 100-63. Plasmid 31, 60-71 
  33. Atlas, R. M. (1993) Handbook of Microbiological Media. CRC Press, Boca Raton. pp. 765 

이 논문을 인용한 문헌 (2)

  1. Kang, Jin-Hae ; Kim, Dae-Hwan ; Lee, Sang-Won ; Kim, Hong-Chul ; Cho, Young-Un ; Gal, Sang-Wan 2010. "Biological Probiotic Properties of Lactobacillus rhamonosus GG-4 Isolated from Infant Feces" 생명과학회지 = Journal of life science, 20(12): 1882~1888 
  2. 2010. "" Korean journal for food science of animal resources = 한국축산식품학회지, 30(4): 634~640 

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