대파(Allium fistulosum L)는 백합과 다년초로서 한국, 일본 중국 등 전 세계적으로 식용으로 재배되어 왔다. 현재까지 대파의 다양한 생리활성이 보고되어 왔으나, 항균 활성에 대해서는 거의 보고되어 있지 않다. 본 연구에서는 대파 식용부위로부터 강력한 항미생물성 steroidal saponins (fistuloside A, B, 및 C)을 분리하였고, 이들의 항균력을 병원성균 및 식품부패균을 대상으로 평가하였다. Fistuloside A 및 C는 강력한 항진균 활성과 항Proteus 세균활성을 나타낸 반면, fistuloside B는 항진균 활성만 인정되었다. 특히 fistuloside C는 $3.1{\sim}6.2{\mu}g/ml$의 MIC (minimal inhibitory concentration) 및 MFC (minimal fungicidal concentration)를 나타내어 강력한 항진균 활성이 진균사멸에 의한 항균효과임을 확인하였다. 본 연구결과는 대파가 미생물 감염증 제어용으로 사용되어 온 근거를 제시하며, 또한 fistuloside C의 항진균제로서의 이용 가능성을 제시하고 있다.
대파(Allium fistulosum L)는 백합과 다년초로서 한국, 일본 중국 등 전 세계적으로 식용으로 재배되어 왔다. 현재까지 대파의 다양한 생리활성이 보고되어 왔으나, 항균 활성에 대해서는 거의 보고되어 있지 않다. 본 연구에서는 대파 식용부위로부터 강력한 항미생물성 steroidal saponins (fistuloside A, B, 및 C)을 분리하였고, 이들의 항균력을 병원성균 및 식품부패균을 대상으로 평가하였다. Fistuloside A 및 C는 강력한 항진균 활성과 항Proteus 세균활성을 나타낸 반면, fistuloside B는 항진균 활성만 인정되었다. 특히 fistuloside C는 $3.1{\sim}6.2{\mu}g/ml$의 MIC (minimal inhibitory concentration) 및 MFC (minimal fungicidal concentration)를 나타내어 강력한 항진균 활성이 진균사멸에 의한 항균효과임을 확인하였다. 본 연구결과는 대파가 미생물 감염증 제어용으로 사용되어 온 근거를 제시하며, 또한 fistuloside C의 항진균제로서의 이용 가능성을 제시하고 있다.
Allium fistulosum L. (Welsh onion) is a perennial herb that is widely cultivated throughout the world, especially in China, Japan and Korea. Although, various activities were reported, little is known about antimicrobial activity of A. fistulosum L.. In this study, strong antimicrobial substances, f...
Allium fistulosum L. (Welsh onion) is a perennial herb that is widely cultivated throughout the world, especially in China, Japan and Korea. Although, various activities were reported, little is known about antimicrobial activity of A. fistulosum L.. In this study, strong antimicrobial substances, fistuloside A, B, and C were isolated from the edible parts of A. fistulosum L. and their antimicrobial activity was evaluated with pathogenic- or food-spoilage microorganism based on disk-diffusion assay, minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) determination. Fistuloside A and fistuloside C showed strong antifungal activity and anti-Proteus activity, while fistuloside B is effective to only fungi. The fistuloside C showed a prominent antifungal activity with $3.1{\sim}6.2{\mu}g/ml$ of MIC and MFC. Our results indicated that fistuloside C has a prominent antifungal activity and support the use of A. fistulosum to treat microbial infection.
Allium fistulosum L. (Welsh onion) is a perennial herb that is widely cultivated throughout the world, especially in China, Japan and Korea. Although, various activities were reported, little is known about antimicrobial activity of A. fistulosum L.. In this study, strong antimicrobial substances, fistuloside A, B, and C were isolated from the edible parts of A. fistulosum L. and their antimicrobial activity was evaluated with pathogenic- or food-spoilage microorganism based on disk-diffusion assay, minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) determination. Fistuloside A and fistuloside C showed strong antifungal activity and anti-Proteus activity, while fistuloside B is effective to only fungi. The fistuloside C showed a prominent antifungal activity with $3.1{\sim}6.2{\mu}g/ml$ of MIC and MFC. Our results indicated that fistuloside C has a prominent antifungal activity and support the use of A. fistulosum to treat microbial infection.
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제안 방법
In this study, antimicrobial substances were isolated from the edible parts of A. fistulosum L. and their antimicrobial activity was evaluated using pathogenic- or food-spoilage microorganism. Our results showed that fistuloside C, a steroidal saponin from A.
대상 데이터
were collected in Kyungpook, Korea in the summer season of 2003. A voucher specimen was deposited at the College of Pharmacy, Yeungnam University, Korea. The dried chopped bulbs and roots (10 kg) were refluxed with hot meth- anolz three times.
Bacterial strains used were Proteus vulgaris KCTC 2433, Pseudomonas aeruginosa KACC 10186, Escherichia coli 0157 ATCC 43895, Staphylococcus epidermidis ATCC 12228, 아了叩tococ아」s mutans JC-2, arid Staphylococcus aureus KCTC 1916. The antibacterial activity was evaluated by disk-diffusion assay determined by growth inhibition zone[llz17].
성능/효과
and their antimicrobial activity was evaluated using pathogenic- or food-spoilage microorganism. Our results showed that fistuloside C, a steroidal saponin from A. fistulosum L, has a prominent antifungal activity, and support the use of A. fistulosum to treat microbial infection as a traditional medicine.
후속연구
Further research on the mode of action of fistuolsides, and modification of fituloside C structure for activity increase or broad antimicrobial spectrum is necessary.
참고문헌 (18)
Al-Waili, N. S. 2004. Investigating the antimicrobial activity of natural honey and its effects on the pathogenic bacterial infections of surgical wounds and conjunctiva. J. Med. Food 7, 210-222
Chen, J.-H., H.-I. Chen, J.-S. Wang, S.-J. Tsai and C. J. Jen. 2000. Effect of welsh onion extracts on human platelet function in vitro. Life Sci. 66, 1571-1579
Iwalokun, B. A., A. Ogunledun, D. O. Ogbolu, S. B. Bamiro and J. Jimi-Omojola. 2004. In vitro antimicrobial properties of aqueous garlic extract against multidrug- resistant bacteria and Candida species from Nigeria. J. Med. Food 7, 327-333
Jiangsu New Medical College. 1986. Zhong-yao-da-ci-dian, pp. 2316-2318, In: Zhong-yao-da-ci-dian. Shanghai Science and Technology Publisher, Shanghai
Lam, Y. W., H. X. Wang and T. B. Ng. 2000. A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum. Biochem. Biophys. Res. Comm. 279, 74-80
Negi, P. S., C. Anandharamakrishnan and G. K. Jayaprakasha, 2003. Antibacterial activity of Aristolochia bracteata root extracts. J. Med. Food 6, 401-403
Phay, N., H. Higashiyama, M. Tsuji, H. Matsuura, Y. Fukushi, A. Yokota and F. Tomita. 1999. An antifungal compound from roots of welsh onion. Phytochemistry 52, 271-274
Sang, S., A. Lao, Y. Wang, C.-K. Chin, R. T. Rosen and C.-T. Ho. 2002. Antifungal constituents from the seeds of Allium fistulosum L. J. Agric. Food Chem. 50, 6318-6321
Seo, G.-W., J.-Y. Cho, J.-H. Kuk, J.-H. Wee, J.-H. Moon, S.-H. Kim and K.-H. Park. 2003. Identification of antioxidative substances in Allium fistulosum L. by GC-MS. Korean J. Food Sci. Technol. 35, 988-993
Shogakukan. 1985. The Dictionary of Chinese drugs. pp. 1599-1600, In: The Dictionary of Chinese drugs Vol. 3. Shanghai Science and Technology Publisher and Shougakukan, Tokyo
Sohn, H.-Y., K. H. Son, C.-S. Kwon, G.-S. Kwon and S. S. Kang. 2004. Antimicrobial and cytotoxic activity of 18 prenylated flavonoids isolated from medicinal plants: Morus alba L., Morus mongolica Schneider, Broussnetia papyrifera (L.) Vent, Sophora flavescens Ait and Echinosophora koreensis Nakai. Phytomedicine 11, 666-672
Yamamoto, Y., S. Aoyama, N. Hamaguchi and G.-S. Rhi. 2005. Antioxidative and antihypertensive effects of welsh onion on rats fed with a high-fat high-sucrose diet. Biosci. Biotecnol. Biochem. 69, 1311-1317
Wang, B.-S., J.-H. Chen, Y.-C. Liang and P.-D. Duh. 2005. Effects of welsh onion on oxidation of low-density lipoprotein and nitric oxide production in macrophage cell line RAW 264.7. Food Chem. 91, 147-155
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