Lee Kye-Nam
(Department of Biology, College of Natural Sciences, Seoul Women's University)
,
Paek Kwang-Hyun
(Plasnix)
,
Ju Won-Tae
(Plasnix)
,
Lee Yeon-Hee
(Department of Biology, College of Natural Sciences, Seoul Women's University)
Atmospheric-pressure cold plasma (APCP) using helium/oxygen was developed and tested as a suitable sterilization method in a clinical environment. The sterilizing effect of this method is not due to UV light, which is known to be the major sterilization factor of APCP, but instead results from the a...
Atmospheric-pressure cold plasma (APCP) using helium/oxygen was developed and tested as a suitable sterilization method in a clinical environment. The sterilizing effect of this method is not due to UV light, which is known to be the major sterilization factor of APCP, but instead results from the action of reactive oxygen radicals. Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae deposited on a nitrocellulose filter membrane or Bacillus subtilis spores deposited on polypropylene plates were exposed to helium/oxygen plasma generated with AC input power at 10 kHz, 6 kV. After Plasma treatment, nitrocellulose filter membranes were overlaid on fresh solid media and CFUs were counted after incubation overnight. D-values were 18 sec for E. coli, 19 sec for S. aureus, 1 min 55 sec for S. cerevisiae, and 14 min for B. subtilis spores. D-values of bacteria and yeast were dependent on the initial inoculation concentration, while the D-value of B. subtilis spores showed no correlation. When treated cells were observed with a scanning electron microscope, E. coli was more heavily damaged than S. aureus, S. cevevisiae exhibited peeling, and B. subtilis spores exhibited shrunken morphology. Results showed that APCP using helium/oxygen has many advantages as a sterilization method, especially in a clinical environment with conditions such as stable temperature, unlimited sample size, and no harmful gas production.
Atmospheric-pressure cold plasma (APCP) using helium/oxygen was developed and tested as a suitable sterilization method in a clinical environment. The sterilizing effect of this method is not due to UV light, which is known to be the major sterilization factor of APCP, but instead results from the action of reactive oxygen radicals. Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae deposited on a nitrocellulose filter membrane or Bacillus subtilis spores deposited on polypropylene plates were exposed to helium/oxygen plasma generated with AC input power at 10 kHz, 6 kV. After Plasma treatment, nitrocellulose filter membranes were overlaid on fresh solid media and CFUs were counted after incubation overnight. D-values were 18 sec for E. coli, 19 sec for S. aureus, 1 min 55 sec for S. cerevisiae, and 14 min for B. subtilis spores. D-values of bacteria and yeast were dependent on the initial inoculation concentration, while the D-value of B. subtilis spores showed no correlation. When treated cells were observed with a scanning electron microscope, E. coli was more heavily damaged than S. aureus, S. cevevisiae exhibited peeling, and B. subtilis spores exhibited shrunken morphology. Results showed that APCP using helium/oxygen has many advantages as a sterilization method, especially in a clinical environment with conditions such as stable temperature, unlimited sample size, and no harmful gas production.
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문제 정의
The objective of this study was to develop an atmospheric-pressure cold plasma (APCP) system that uses helium/oxygen (He/O2)and is able to effectively penetrate into complicated structures such as tubes and artificial organs without producing harmful residues or increasing temperature. To accomplish this, helium gas was chosen because of its lack of chemical activity and its lack of specific effects on microorganisms despite the intense ion and electron bombardments; oxygen gas was used to increase the sterilizing effect.
대상 데이터
The potential effect of density on the sterilization ofB. subtilis spores was tested. The same numbers of B.
성능/효과
subtilis population or for other reasons that have not yet been elucidated. In conclusion, APCP using He/02 can efficiently sterilize various microorganisms, including B. subtilis spores, without producing any harmful residues. It requires a short treatment time (less than 20 sec for bacteria, less than 2 min for yeast, and 14 min for bacterial spores) and does not expose materials to high temperatures or pressures, both of which are produced from classical sterilization methods such as autoclaving, chemical treatment, and high doses of irradiation.
, 2003). Third, the sterilization kinetics of B. subtilis spores by APCP using He/O2 showed a biphasic curve with a faster decrease in spore viability at the earliest stage, followed by a slower decrease at later stages. Similar biphasic sterilization phenomena have been reported by others (Lerouge et al.
This study showed that APCP using He/O2 generates a low level of UV while producing reactive oxygen radicals that likely act as the main factor for sterilization; these reactive oxygen radicals carry the advantage of being capable of accessing the interiors of complicated structures such as tubes and artificial organs. Moreover, this new system did not produce excessive heat when used for up to 1 h of treatment.
후속연구
This finding supported the aforementioned hypothesis, which proposed that He/02 plasma showed a sterilizing effect via oxygen radicals that can access and directly attack the cell wall. Further study is needed to determine how oxygen radicals can peel off the bacterial cell wall or shrink spores, resulting in the death of the microorganisms.
참고문헌 (14)
Affatato, S., G. Bersaglia, I. Foltran, P. Taddei, G. Fini, and A. Toni. 2002. The performance of gamma- and EtO-sterilised UHWMPE acetabular cups tested under severe simulator conditions. Biomaterials 23, 4839-4846
Hury, S., D.R. Vidal, F. Desor, J. Pelletier, and T. Lagarde. 1998. A parametric study of the destruction efficiency of Bacillus spores in low pressure oxygen-based plasmas. Letters Appl. Microbiol. 26, 417-421
Lara, J., P.S. Fernandez, P.M. Periago, and A. Palop. 2002. Irradiation of spores of Bacillus cereus and Bacillus subtilis with electron beams. Innov. Food Sci. Emerg. Technol. 3, 379-384
Lerouge, S., M.R. Wertheimer, R. Marchand, M. Tabrizian, and L.. Yahia. 2000a. Effect of gas composition on spore mortality and etching during low-pressure plasma sterilization. J. Biomed. Mater. Res. 51, 128-135
Lerouge, S., C. Guignot, M. Tabrizian, D. Ferrier, N. Yagoubi, and L.. Yahia. 2000b. Plasma-based sterilization: effect on surface and bulk properties and hydrolytic stability of reprocessed polyurethane electrophysiology catheters. J. Biomed. Mater. Res. 52, 774-782
Lucas, A.D., K. Merritt, Y.M. Hitchins, T.O. Woods, S.G. Nclvlamee, D.B. Lyle, and S.A. Brown. 2003. Residual ethylene oxide in medical devices and device material. J. Biomed. Mater. Res. 66, 548-552
Moisan, M., J. Barbeau, M.C. Crevier, J. Pelletier, N. Philip, and B. Saoudi. 2002. Plasma sterilization: Methods and mechanisms. Pure Appl. Chem. 74, 349-358
Noyce, J.O. and J.P. Hughes. 2002. Bactericidal effects of negative and positive ions generated in nitrogen on Escherichia coli. J. Electrostatics 54, 179-187
Oshima, T. and M. Sato. 2004. Bacterial sterilization and intracellular protein release by a pulsed electric field. Adv. Biochem. Eng. Biotechnol. 90, 113-33
Park, J.C., B.J Park, D. Han, D.H. Lee, I. Lee, S.O. Hyun, M.S. Chun, K.H. Chung, M. Aihara, and K. Takatori. 2004. Fungal sterilization using microwave-induced argon plasma at atmospheric pressure. J. Microbiol. Biotechnol. 14, 188-192
Spilimbergo, S., F. Dehghani, A. Bertucco, and N.R. Foster. 2003. Inactivation of bacteria and spores by pulse electric field and high pressure $CO_2$ at low temperature. Biotechnol. Bioeng. 82, 118-125
Takeshita, K., J. Shibato, T. Sarneshima, S. Fukunaga, S. Isobe, K. Arihara, and M. Itoh. 2003. Damage of yeast cells induced by pulsed light irradiation. Int. J. Food Microbiol. 85, 151-158
Uemura, K. and S. Isobe. 2002. Developing a new apparatus for inactivating Escherichia coli in saline water with high electric field AC. J. Food Eng. 53, 203-207
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