[논문]Phage Conversion for β-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods

Lee, Young-Duck; Park, Jong-Hyun

doi:10.4014/jmb.1508.08042

Phage Conversion for β-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods 원문보기

Journal of microbiology and biotechnology, v.26 no.2, 2016년, pp.263 - 269

Lee, Young-Duck (Department of Food Science and Engineering, Seowon University) , Park, Jong-Hyun (Department of Food Science and Biotechnology, Gachon University)

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Temperate phages have been suggested to carry virulence factors and other lysogenic conversion genes that play important roles in pathogenicity. In this study, phage TEM123 in wild-type Staphylococcus aureus from food sources was analyzed with respect to its morphology, genome sequence, and antibiotic resistance conversion ability. Phage TEM123 from a mitomycin C-induced lysate of S. aureus was isolated from foods. Morphological analysis under a transmission electron microscope revealed that it belonged to the family Siphoviridae. The genome of phage TEM123 consisted of a double-stranded DNA of 43,786 bp with a G+C content of 34.06%. A bioinformatics analysis of the phage genome identified 43 putative open reading frames (ORFs). ORF1 encoded a protein that was nearly identical to the metallo-β-lactamase enzymes that degrade β-lactam antibiotics. After transduction to S. aureus with phage TEM123, the metallo-β-lactamase gene was confirmed in the transductant by PCR and sequencing analyses. In a β-lactam antibiotic susceptibility test, the transductant was more highly resistant to β-lactam antibiotics than S. aureus S133. Phage TEM123 might play a role in the transfer of β-lactam antibiotic resistance determinants in S. aureus. Therefore, we suggest that the prophage of S. aureus with its exotoxin is a risk factor for food safety in the food chain through lateral gene transfer.

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The type strain S. aureus KCCM12103 was purchased from the Korean Culture Center of Microorganisms. Wild-type S.

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Phage Conversion for β-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods 원문보기

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Phage Conversion for β-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods 원문보기

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