[논문]ISSR 분자 마커를 이용한 왕대속 대나무의 유전적 다양성 및 계통 관계

이송진; 허만규; 허홍욱

doi:10.5352/jls.2007.17.11.1482

ISSR 분자 마커를 이용한 왕대속 대나무의 유전적 다양성 및 계통 관계
Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by ISSR Markers 원문보기

생명과학회지 = Journal of life science, v.17 no.11 = no.91, 2007년, pp.1482 - 1487

이송진 (부산대학교 생물학과) , 허만규 (동의대학교 분자생물학과) , 허홍욱 (부산대학교 생물학과)

초록
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ISSR분자 마커를 이용하여 왕대속 대나무의 유연관계를 분석 실시하였다. 전세계적으로 왕대속에 속하는 4종은 경제학적, 생태학적으로 중요한 식물 자원중 하나이다. 총 11개의 ISSR 프라이머 중 9개의 프라이머에서 증폭이 일어 났으며 총 64개의 밴드를 확인 할 수 있었다. ISSR분석결과 왕대속 4종의 대나무에서 70.49%인 43개의 다형현상이 나타났다. 4개의 분류군으로 분류된 왕대속 대나무의 집단내 다양성(Hs)은 0.092,집단간 다양성(Gst)은 0.499로 나타났다. 각 종에 대한 유전자 유동(Nm)은 0.559로 나타났다. 따라서 왕대속에 속하는 4종의 유전자 유동(Nm)은 아주 낮음을 알 수 있었다 4종의 분류군에 대한 유전자 다양성(Ht)은 0.291로 낮게 나타났으며 ISSR 마커로 명확하게 그들은 분류가 되었다. 또한 왕대속의 4종은 단일계통임을 확인할 수 있었다.

Abstract ▼ AI-Helper

Four taxa of the genus Phyllostachys were analysed with PCR-based molecular inter-simple sequence repeat (ISSR) determine markers to determine their phylogenetic relationships. Many species of this genus are regarded as economical and ecologically important in the world. With the nine primers screened, ISSR assay generated a total of 64 reproducible bands. Analysis of ISSR from individual plants of genus Phyllostachys resulted in 43 polymorphic bands with 70.49%. When species were grouped by four taxa, within group diversity was 0.092 (Hs), while among group diversify was 0.499 (Gst) on a per locus basis. The estimated gene flow (Nm) between the pairs of species was 0.559. Hence, we can expect weak or low gene flow among species. Total mean genetic diversity ($H_T$) for four taxa was 0.291. The ISSR data allowed us to resolve well-supported clades in the genus Phyllostachys. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic.

주제어

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문제 정의

Therefore, the objectives of this study were to estimate how much genetic diversity is maintained in genus Phyllostachys and to describe germ-plasm classification within genus.

이론/모형

A phylogenetic tree was constructed by the neighbor-joining (NJ) method [18] using the NEIGHBOR program in PHYLIP version 3.57 [5].
Clustering of four populations per species, using the NJ algorithm, was performed based on the matrix of calculated distances (Fig. 1). The four taxa of genus Phyllostachys analyzed were distinctly related to a monophyletic.

성능/효과

1). The four taxa of genus Phyllostachys analyzed were distinctly related to a monophyletic. P.

후속연구

nigra, and thus deserve taxonomic treatment at subspecies or even at species level. Although the size of sampling was not large enough for the genus Phyllostachys, the analyses of RAPDs will certainly provide an enhanced view on the phylogeny of this genus. In addition, It is necessary to identify taxa that additional molecular experiments such as AFLP, SSR, and ITS.
Although the size of sampling was not large enough for the genus Phyllostachys, the analyses of RAPDs will certainly provide an enhanced view on the phylogeny of this genus. In addition, It is necessary to identify taxa that additional molecular experiments such as AFLP, SSR, and ITS.

참고문헌 (21)

Bartish, I. V., L. P. Garkava, K. Rumpunen and H. Nybom. 2000. Phylogenetic relationships and differentiation among and within populations of Chaenomeles Lindl. (Rosaceae) estimated with RAPDs and isozymes. Theor. Appl. Genet. 101, 554-563.

상세보기
Bowman, K. D., K. Hutcheson, E. P. Odum and L. R. Shenton. 1971. Comments on the distribution of indices of diversity. Stat. Ecol. 3, 315-359.
Esselman, E. J., L. Jianqiang, D. J. Crawford, J. L. Winduss and A. D. Wolfe. 1999. Clonal diversity in the rare Calamagrostis porteri ssp. Insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Molecular Ecology 8, 443-451.

상세보기
Fang, D. Q. and M. Roose. 1997. Identification of closely related citus cultivars with and inter-simple sequence repeat markers. Theoretical and Applied Genetics 95, 408-417.

상세보기
Felsenstein, J. 1993. PHYLIP (Phylogeny Inference Package) version 3.5s. Distributed by the author. Department of Genetics, Univ. Washington, Seattle.
Godwin, I. D., E. A. B. Aitken and L. W. Smith. 1997. Application of and inter simple sequence repeat (ISSR) markers to plant genetics. Electrophresis 18, 1524-1528.

상세보기
Hamrick, J. L. and M. J. W. Godt. 1989. Allozyme diversity in plant species, pp. 304-319 in Plant Population Genetics, Breeding and Genetic Resources, edited by A. H. D. Brown, M. T. Clegg, A. L. Kahler and B. S. Weir, Sinauer Associates, Sunderland, MA.
Hamrick, J. L., Godt, M. J. W. and S. L. Sherman-Broyles. 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6, 95-124.

상세보기
Huh, M. K. 1999. Genetic diversity and population structure of Korean Alder (Alnus japonica : Betulaceae). Can. J. For. Res. 29, 1311-1316.

상세보기
Huh, M. K. and H. W. Huh. 2001. Genetic diversity and phylogenetic relationships in alder, Alnus firma, revealed by AFLP. Korean J. Plant Biol. 44, 33-40.
Huh, M. K. and H. W. Huh. 2002. Genetic diversity and population structure of Pseudosasa japonica (Bambusaceae) in Korea. Bamboo Sci. & Culture 16, 9-17.
Lee, Y. N. 1997. Flora of Korea. Kyo-Hak Publishing Co, Seoul, Korea.
Li, A. and S. Ge. 2001. Genetic variation and clonal diversity of Psammochloa villosa (Poaceae) dected by ISSR markers. Annulas of Botany 87, 585-590.

상세보기
Nei, M and W. H. Li. 1979. Mathematical model for studying genetical variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 74, 5267-5273.
Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70, 3321-3323.

상세보기
Paul, S. P., F. N. Wachira, W. Powell and R. Waugh. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor. Appl. Genet. 94, 255-263.

상세보기
Qian, W., Ge, S. and D. Y. Hong. 2001. Genetic variation within and among populations of wild rice Oryza granulata from China detected by RAPD and ISSR markers. Theor. Appl. Genet. 102, 440-449.

상세보기
Saitou, N. and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406-425.

상세보기
Tsumura, Y., K. Ohba and S. H. Strauss. 1996. Diversity anf inheritance of inter-simple sequence repeat polymorphism in Douglas-fir (Pseudotsuga menziesii) and sugi (Cryptomeria japonica). Theor. Appl. Genet. 92, 40-45.

상세보기
Wolfe, A. D. and A. Liston. 1998. Contributions of PCRbased methods to plant systematics and evolutionary biology. pp. 43-86, In: Soltis D.E.., P.S.. Soltis and J. J. Doyle (eds.): Molecular systematics of Plants II, Kluwer.
Yeh, F. C., R. C. Yang and T. Boyle. 1999. POPGENE version 1.31, Microsoft Windows-based Freeware for Population Genetic Analysis.

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