[논문]Hydrolysis of Olive Oil Catalyzed by Surfactant-Coated Candida rugosa Lipase in a Hollow Fiber Membrane Reactor

Song, Bao-Dong; Ding, Hui; Wang, Shi-Chang

doi:10.1007/bf03028636

Hydrolysis of Olive Oil Catalyzed by Surfactant-Coated Candida rugosa Lipase in a Hollow Fiber Membrane Reactor 원문보기

Biotechnology and bioprocess engineering : Bbe, v.12 no.2, 2007년, pp.121 - 124

Song, Bao-Dong (Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University) , Ding, Hui (Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University) , Wang, Shi-Chang (Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University)

Abstract ▼ AI-Helper

In this study, we investigated the hydrolysis of olive oil catalyzed by a surfactant-coated Candida rugosa lipase in a hydrophilic polyacrylonitrile hollow fiber membrane reactor and then compared the results to those using the native lipase. The organic phase was passed through the hollow inner fibers of the reactor, and consisted of either the coated lipase and olive oil dissolved in isooctane or the coated lipase dissolved in pure olive oil. The aqueous phase was pumped through the outer space. After 12 h and with conditions of $30^{\circ}C$, 0.12 mg enzyme/mL and 0.62 M olive oil, the substrate conversion of the coated lipase reached 60%. This was twice the conversion for the same amount of native lipase that was pre-immobilized on the membrane surface. When using pure olive oil, after 12 h the substrate conversion of the coated lipase was 50%, which was 1.4 times higher than that of the native lipase.

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참고문헌 (13)

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Hydrolysis of Olive Oil Catalyzed by Surfactant-Coated Candida rugosa Lipase in a Hollow Fiber Membrane Reactor 원문보기

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