Hydrogen cyanide(HCN), formed from pyrolysis of various nitrogenous compounds such as protein, amino acids and nitrate in tobacco, is present in both the particulate phase and vapor phase of cigarette smoke. Typically the determination of HCN in cigarette smoke has been done through colorimetric and...
Hydrogen cyanide(HCN), formed from pyrolysis of various nitrogenous compounds such as protein, amino acids and nitrate in tobacco, is present in both the particulate phase and vapor phase of cigarette smoke. Typically the determination of HCN in cigarette smoke has been done through colorimetric and electrochemical techniques, such as fluorescence spectrometry, UV-spectrophotometry (UV), continuous flow analyzer (CFA), capillary GC-ECD and ion chromatography (IC). Most of these techniques are known to be time-consuming and some of them lack specificity or sensitivity. The available results from both our laboratory and reported literatures for 2R4F Kentucky reference cigarette, smoked under ISO condition, show a relatively wide variation ranging from 100 to 120 ug/cig of HCN. Especially, the precision and accuracy of the analytical results of HCN tend to get worse in low tar cigarettes and under intense smoking condition. In this paper, a more optimized analytical methods than previous ones are suggested. This method shows lower detection limit and has improved precision and accuracy, so it is applicable for wide tar level cigarettes under intense smoking condition as well as under ISO smoking condition. Important features of this method are improved sample collection and quantification systems such as the number of trapping units, volume, temperature and type of trapping solution. To avoid volatilization loss of HCN in analyzing mainstream smoke, it is highly recommended that pH values of trapping solutions should be maintained over 11 and cold traps should be used in collecting mainstream smoke.
Hydrogen cyanide(HCN), formed from pyrolysis of various nitrogenous compounds such as protein, amino acids and nitrate in tobacco, is present in both the particulate phase and vapor phase of cigarette smoke. Typically the determination of HCN in cigarette smoke has been done through colorimetric and electrochemical techniques, such as fluorescence spectrometry, UV-spectrophotometry (UV), continuous flow analyzer (CFA), capillary GC-ECD and ion chromatography (IC). Most of these techniques are known to be time-consuming and some of them lack specificity or sensitivity. The available results from both our laboratory and reported literatures for 2R4F Kentucky reference cigarette, smoked under ISO condition, show a relatively wide variation ranging from 100 to 120 ug/cig of HCN. Especially, the precision and accuracy of the analytical results of HCN tend to get worse in low tar cigarettes and under intense smoking condition. In this paper, a more optimized analytical methods than previous ones are suggested. This method shows lower detection limit and has improved precision and accuracy, so it is applicable for wide tar level cigarettes under intense smoking condition as well as under ISO smoking condition. Important features of this method are improved sample collection and quantification systems such as the number of trapping units, volume, temperature and type of trapping solution. To avoid volatilization loss of HCN in analyzing mainstream smoke, it is highly recommended that pH values of trapping solutions should be maintained over 11 and cold traps should be used in collecting mainstream smoke.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
문제 정의
simultaneously. Therefore, an optimized analytical procedure for HCN in cigarette smoke has been set up and tested in this work. The results are as follows;
제안 방법
A Jasco UV-560 double beam UV-visible spectrophotometer with a fixed bandwidth and data processing capacity was used for this experiment. The zero-order adsorption spectra were recorded over the wavelength range of 200 ~800 nm, against a solvent blank, in quartz cuvette with 1 cm diameter.
Chromatography was performed with Agilent technologies series 6890N equipment including a 7683 automated liquid sample-injection system, a split/splitless injector, a 30 m x 320 um x 0.25 um nominal HP~5 (5 %-pheny methyl siloxane) capillary column and a ECD detector controlled by the Agilent Chemstation software. The carrier gas was helium at a constant flow rate of 0.
참고문헌 (6)
Brunnemann, K. D., Yu, L. and Hoffmann, D. (1977) Chemical studies on tobacco smoke: Gas chromatographic determination of hydrogen cyanide and cyanogens in tobacco smoke. J. Anal. Toxicol. 1(1): 38-42
Collins, P. F., Sarji, N. M. and William, J. F. (1973) A trapping system for the combined determination of total HCN and total gas phase aldehydes in cigarette smoke. Beitr. Tobakforsch. Int. 7(2): 73-78
Juan, X., Hongwu, T., Xiangyang, Y., Shan, D., Zhongda, Y. and Shaomin, L. (2006) Sensitive determination of cyanide in cigarette smoke by capillary GC with a microECD. Chromatographia, 64(9): 609-612
P. X. Chen, S. C. and Moldoveanu (2003) Mainstream smoke chemical analyses for 2R4F Kentucky reference cigarette. Beitr. Tobakforsch Int, 20(7): 448-458
Rickert, W. S. and Stockwell, P. B. (1979) Automated determination of hydrogen cyanide, acrolein and total aldehydes in the gas phase of tobacco smoke. J. Automat. Chem. 1: 152-154
AI-Helper
※ AI-Helper는 부적절한 답변을 할 수 있습니다.