[논문]Physiological Damages and Biochemical Alleviation to Ozone Toxicity in Five Species of genus Acer

Han, Sim-Hee; Kim, Du-Hyun; Lee, Kab-Yeon; Ku, Ja-Jung; Kim, Pan-Gi

[국내논문] Physiological Damages and Biochemical Alleviation to Ozone Toxicity in Five Species of genus Acer 원문보기

韓國林學會誌 = Journal of Korean Forest Society, v.96 no.5 = no.174, 2007년, pp.551 - 560

Han, Sim-Hee (Dept. of Forest Genetic Resources, Korea Forest Research Institute) , Kim, Du-Hyun (Dept. of Forest Genetic Resources, Korea Forest Research Institute) , Lee, Kab-Yeon (Dept. of Forest Genetic Resources, Korea Forest Research Institute) , Ku, Ja-Jung (Dept. of Forest Genetic Resources, Korea Forest Research Institute) , Kim, Pan-Gi (Dept. of Forest Resources and Environment, Sangju National University)

Abstract ▼ AI-Helper

We investigated physiological damages and biochemical alleviation of five species of genus Acer under ozone fumigation in order to assess their tolerant ability against ozone toxicity. At the end of 150 ppb $O_3$ fumigation, photosynthetic characteristics were measured, and chlorophyll contents, malondialdehyde (MDA) and antioxidative enzyme activities were analyzed in the leaves of five maple trees (Acer buergerianum, A. ginnala, A. mono, A. palmatum, and A. palmatum var. sanguineum). The reduction of chlorophyll (chl) a in ozone-exposed plants was 16.8% (A. buergerianum) to 26.7% (A. ginnala) of control plants. For the content of chi b, A. ginnala and A. palmatum var. sanguineum represented the high reduction of 26.3% and 23.6%, respectively. The highest reduction on the chi a:b ratio was observed in the leaves of A. palmatum. The reduction of net photosynthesis in five species varied from 2.4% to 37.6%. Among five species, A. ginnala showed remarkable reduction (37.6%) for net photosynthesis in comparison with control. Carboxylation efficiency differed significantly (P < 0.05) among species and between control and ozone treatment. The reduction of carboxylation efficiency was the highest in the leaves of A. ginnala (44.7%). A. palmatum var. sanguineum showed the highest increase (41.7%) for MDA content. The highest increase of superoxide dismutase (SOD) activity represented in A. palmatum (26.1%) and the increase of ascorbate peroxidase (APX) activity ranged from 16.5% (A. ginnala) to 49.1% (A. palmatum var. sanguineum). A. mono showed the highest increase (376.6%) of glutathione reductase (GR) activity under ozone fumigation and A. buergerianum also represented high increase (42.3%) of GR activity. Catalse (CAT) activity increased in the leaves of A. ginnala, A. palmatun and A. palmatum var. sanguineum under ozone exposure, whereas A. buergerianum and A. mono decreased in comparison with control plants. In conclusion, physiological markers such as chlorophyll content and photosynthesis that responded sensitively to $O_3$ in maple trees were considered as the very important indicators in order to evaluate the tolerance against $O_3$ stress, and parameters were closely related with each other. Among anti oxidative enzymes, SOD and APX might be contributed to alleviate to $O_3$ toxicity through the increase of activity in all maple trees. Therefore, these compounds can be used as a biochemical maker to assess the stress tolerance to $O_3$.

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문제 정의

Therefore, this study was undertaken to investigate cellular responses to ozone as mechanism for oxidant impact on photosynthesis in seedlings of maple trees. Specifically, our objectives are to determine the effects of ozone exposure on photosynthetic pigments, photosynthesis, antioxidant enzyme activities and lipid peroxidation.

데이터처리

To compare the effect on control and O₃ treatment, ANOVA was performed on experimental data (statistical significance, P<0.05), and Duncan's multiple range tests were performed. Statistical analyses were performed using the statistical package SAS System for Windows, Version 8.

성능/효과

In addition, species x ozone treatment interaction was observed for net photosynthesis. The reduction rate of net photosynthesis in the seedlings of five species varied from 2.4% to 37.6%, Among five species, A. ginnala showed remarkable red니ction rate (37.6%) for net photosynthesis in comparison with control But net photosynthesis of A. palmatum didn't decrease significantly under ozone fumigation.
However, there wasn't significant difference between control and ozone treatment (Table 2), and species x ozone treatment interaction wasn't also observed for SOD activity. A. palmatum showed the highest increase rate of SOD activity (26.1%) and A. mono didn't 아low significantly the increase of SOD activity.
05), and species x ozone treatment interaction was also observed for GR activity. In special, A. mono showed the highest increase rate (376.6%) of GR activity under ozone fumigation, and A. buergerianum also represented high increase rate (42.3%) of GR activity. On the contrary, A.
. In our results, the contents of chlorophyll and carotenoid significantly decreased in the leaves of five species treated with O₃, and chi a was more sensitive than chi b under ozone fumigation (Table 1). Reduction in the chi content of leaves, following exposure of plants to ozone, has been reported in many species such as Picea abies (Robinson and Wellbum, 1991), and Pinus ponderosa (Anderson et al.
In conclusion, physiological markers such as chlorophyll content and photosynthesis that responded sensitively to O₃ in maple trees were considered as the very important indicators in order to evaluate the tolerance against O₃ stress, and parameters were closely related with each other. MDA content wasn't feasible as a indicator on the physiological damage due to the irregular responses.

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