[논문]L-Carnitine Reduces Obesity Caused by High-Fat Diet in C57BL/6J Mice

Mun, Eun-Gyeng; Soh, Ju-Ryoun; Cha, Youn-Soo

[국내논문] L-Carnitine Reduces Obesity Caused by High-Fat Diet in C57BL/6J Mice 원문보기

Food science and biotechnology, v.16 no.2 = no.74, 2007년, pp.228 - 233

Mun, Eun-Gyeng (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University) , Soh, Ju-Ryoun (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University) , Cha, Youn-Soo (Department of Food Science and Human Nutrition, College of Human Ecology, Chonbuk National University)

Abstract ▼ AI-Helper

This study evaluated the effects of carnitine supplementation on obesity caused by a high-fat diet in C57BL/6J mice. The mice were fed a normal diet (ND), high-fat diet (HD), or carnitine-supplemented (0.5% of diet) high-fat diet (HDC) for 12 weeks. The results showed that body weight, energy intake, and feed intake were lower in the HDC group than the control groups. Acid-soluble acylcarnitine (A SAC), acid-insoluble acylcarnitine (AIAC), and total carnitine (TCNE) in the serum and liver were significantly higher in the HDC group. Hepatic carnitine palmitoyl transferase-I activity was significantly higher in the HDC group than the control groups. Acyl-coA synthetase (ACS) and carnitine palmitoyl transferase-I (CPT-I) mRNA expression in the liver was highest in the HDC group, however hepatic acetyl-coA carboxylase (ACC) mRNA expression in this group was lowest. Serum leptin levels and abdominal fat weight were lowest in the HDC group. We concluded that L-carnitine supplementation diminished the risk of obesity caused by a high-fat diet.

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가설 설정

1)Mean±SD of 6 mice per group.
2) Mean±SD of 6 mice per group.
2) Mean±SD of 6 mice per group.

제안 방법

0% agarose gel electrophoresis. By this method we confirmed the expression of acyl-coA synthetase (ACS), carnitine palmitoyl transferase-I (CPT- I), acetyl-coA carboxylase (ACC), and acyl-coA oxidase (ACO). The relative intensity of all mRNAs was analyzed using the AlphaEaseFC software (Alpha Imotech Corporation, San Leandro, CA, USA).
Japan). The animals were adapted to a chow diet for 1 week, and then divided into 3 groups of 10 each using a randomized block design; a normal diet group (ND), high-fat diet group (HD), and camitine-supplemented high-fat diet (HDC) group (Table 1; Research Diet, New Brunswick, NJ, USA). Each group was fed its respective AIN-93 modified diet for 12 weeks (Table 1).
reagent. The concentration was confirmed with a spectrophotometer, and reverse transcription-polymerase chain reaction (RT-PCR) was carried out using a one-step RT-PCR kit (ABgene, NY USA) for cDNA synthesis on an RT-PCR machine (MWG-Biotech, High Point, NC, USA). P-Actin cDNA was also prepared as a control.
In this study, we investigated the effects of L-camitine supplementation on obese mice with type-II diabetes. This study confirmed the relationship between dietary carnitine and obesity in C₅₇BL/6J mice, a strain with a propensity for high-fot diet-induced obesity, as shown by carnitine status and obesity indexes, s_니ch as serum lipid profiles, leptin levels, and the fat weight of each organ.

대상 데이터

Livers were prepared using 29 volumes of 0.3 M-perchloride acid (PCA). Homogenates were centrifoged at l, 500xg and separated from the supernatant.
Thirty male C₅₇BL/6J mice, aged _사 weeks, were purchased from Charles River Laboratories (Tokyo, Japan). The animals were adapted to a chow diet for 1 week, and then divided into 3 groups of 10 each using a randomized block design; a normal diet group (ND), high-fat diet group (HD), and camitine-supplemented high-fat diet (HDC) group (Table 1; Research Diet, New Brunswick, NJ, USA).

데이터처리

2)values with different symbols within the same row are significantly different atp<0.05 by Duncan's multiple range test.
3) values with different symbols within the same row are significantly different atp<0.05 by Duncan's multiple range test.
3)Values with different symbols within the same row are significantly different at/X0.05 by Duncan's multiple range test.
values with different symbols within the same row are significantly different atp<0.05 by Duncan's multiple range test.
standard deviation. SAS software was used to detemiine significant differences between each group by ANOVA and Duncan's multiple range test at y?<0.05 levels.
Mean±SD of 6 mice per group. Values with different symbols within the same row are significantly different at p<0.05 by Duncan's multiple range test.
Mean±SD of 6 mice per group. Values with different symbols within the same row are significantly different at pv0.05 by Duncan's multiple range test.
4. mRNA expression in liven Values with different symbols within the same row are significantly different at p<0.05 by Duncan's multiple range test. ACS, acyl-coenzyme A synthetase; CPT-I, carnitine palmitoyltransferase-I; ACC, acetyl-coenzyme A carboxylase; ACO, acyl-coenzyme A oxidase.

이론/모형

Homogenates were centrifoged at l, 500xg and separated from the supernatant. Blood and tissues were prepared for carnitine analysis using the Cederblad and Lindstedt method (16). Acid-insoluble acylcamtine (AIAC) was removed using perchloric acid precipitation and centrifugation.
serum and liver. Triglycerides were measured using a commercial kit (Asan Pharm. Co., Seoul, Korea) by the lipase-glycerol phosphate method. High density-lipoprotein cholesterol (HDL-C) concentration was analyzed by phosphotungstic acid-Mg using a commercial kit (Asan Pharm.

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