선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- This was concluded with a final extension for 7 min at 72 ℃.Amplicons were separated in 1 % agarose gels in 0.5 TBE buffer at 100 V for 30 min, stained withethidium bromide and visualised under UV light, GAPDH was used as an internal control to evaluate relative expressions of TNF- a and IL-4.
- Table 1. Oligonucleotide primers used for PCR in this study.
대상 데이터
- . Dulbecco's Modified Eagle's Medium (DMEM) containing L-glutamine (200 mg/L) and FBS were purchased from Hyclone. TNF ELISA kit (BD OptEIATM Rat TNF ELISA Set), IL-4 ELISA kit (BD OptEIATM Rat Ⅱ.
이론/모형
- Cells were pretreated with Dictamni Radicis Cortex for 1 h and then challenged PMA plus A23187 for 8 h. IL-4 concentrations were measured from cell supernatants using ELISA method. Vertical bars represent as the mean SEM from 4 wells
- Cells were pretreated with Dictamni Radicis Cortex for 1 h and then challenged PMA plus A23187 for 8 h. TNF-a concentrations were measured from cell supernatants using ELISA method. Vertical bars represent as the mean SEM from 4 wells.
- protein levels by ELISA method. When RBL-2H3 cells were treated with 1.
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