개 혈액에서 Multiplex Nested PCR기법을 이용한 Brucella spp. 및 Leptospira interrogans 검출 Detection of Brucella spp. and Leptospira interrogans in the Canine Blood by Multiplex Nested PCR원문보기
본 연구는 특이적인 임상증상이 없는 360두의 개를 대상으로 multiplex nested PCR 기법을 이용하여 Brucella spp. 및 Leptospira interrogans를 검출하였다. Brucella spp.는 총4두 (1.1%, 암컷 2두, 수컷 2두)가 검출되었고, Leptospira interrogans는 59두 (16.4%, 암컷31두, 수컷28두)에서 검출되었다. 결론적으로 Brucella spp.의 검출률은 낮은 반면 Leptospira interrogans는 높았다. 또한 multiplex nested PCR기법은 무증상의 개 혈액에서 Brucella spp. 및 Leptospira interrogans를 조기에 검출하는데 빠르고 편리한 기법으로 판단된다.
본 연구는 특이적인 임상증상이 없는 360두의 개를 대상으로 multiplex nested PCR 기법을 이용하여 Brucella spp. 및 Leptospira interrogans를 검출하였다. Brucella spp.는 총4두 (1.1%, 암컷 2두, 수컷 2두)가 검출되었고, Leptospira interrogans는 59두 (16.4%, 암컷31두, 수컷28두)에서 검출되었다. 결론적으로 Brucella spp.의 검출률은 낮은 반면 Leptospira interrogans는 높았다. 또한 multiplex nested PCR기법은 무증상의 개 혈액에서 Brucella spp. 및 Leptospira interrogans를 조기에 검출하는데 빠르고 편리한 기법으로 판단된다.
This study examined the prevalence of Brucella spp. and Leptospira interrogans in 360 clinically healthy dogs using multiplex nested PCR. Four dogs (1.1%, 2 females and 2 males) tested positive to Brucella spp. by multiplex nested PCR. Fifty nine (16.4%, 31 females and 28 males) of 360 dogs tested p...
This study examined the prevalence of Brucella spp. and Leptospira interrogans in 360 clinically healthy dogs using multiplex nested PCR. Four dogs (1.1%, 2 females and 2 males) tested positive to Brucella spp. by multiplex nested PCR. Fifty nine (16.4%, 31 females and 28 males) of 360 dogs tested positive L. interrogans. In 1 and 2 of the samples that tested positive to Brucella spp. and L. interrogans, the partial sequences of the virB1 and 16S rRNA genes were identified by direct sequence analysis, respectively. In conclusion, prevalence of Brucella spp. and L. interrogans by multiplex nested PCR revealed low and high, respectively. Multiplex nested PCR is can be useful for early detection of Brucella spp. and L. interrogans in the canine blood from asymptomatic dogs.
This study examined the prevalence of Brucella spp. and Leptospira interrogans in 360 clinically healthy dogs using multiplex nested PCR. Four dogs (1.1%, 2 females and 2 males) tested positive to Brucella spp. by multiplex nested PCR. Fifty nine (16.4%, 31 females and 28 males) of 360 dogs tested positive L. interrogans. In 1 and 2 of the samples that tested positive to Brucella spp. and L. interrogans, the partial sequences of the virB1 and 16S rRNA genes were identified by direct sequence analysis, respectively. In conclusion, prevalence of Brucella spp. and L. interrogans by multiplex nested PCR revealed low and high, respectively. Multiplex nested PCR is can be useful for early detection of Brucella spp. and L. interrogans in the canine blood from asymptomatic dogs.
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제안 방법
interrogans, respectively (2, 3, 8). For the first-round amplification, the PCR reaction was performed in a 20 jil reaction mixtures, containing 5 Ong template DNA, 20 pmol of each primer, 10 mM of dNTP mixtures and 1 units of prime Taq DNA polymerase (Genet Bio, Ltd., Korea). The cycling conditions were 95℃ or 5 min, 40 cycles of 95℃ for 30 sec, 52℃ for 1 min, 72℃ for 1 min and a final extension of 72℃ for 1 min.
대상 데이터
Three hundreds and sixty dogs (186 females and 174 males) in the 2 training centers for hunting (one Clumcheon area and one Namyangju area) and stray dogs in the Daejeon area were examined from 2004 to 2006 in Korea. Blood samples were collected from the jugular to a tube containing EDTA and collected samples were then stored at -20℃ until DNA isolation.
참고문헌 (8)
Corbel MJ. Brucellosis: an overview. Emerg Infect Dis 1997 3: 213-221
Fukunaga M, Horie I, Okuzako N, Mifuchi I.Nucleotide sequence of a 16S rRNA gene for Leptospira interrogans serovar canicola strain Moulton. Nucleic Acids Res 1990 18: 366
Kim S, Lee DS, Suzuki H, Watarai M. Detection of Brucella canisand Leptospira interrogans in canine semen by multiplex nested PCR. J Vet Med Sci 2006 68:615-618
Ryu E, Suh IS. Studies on leptospiral antibody in canine and murine species. Korean J Vet Res 1971; 11: 41-47
Seo IS.Serological survey on the leptospiral antibody in domestic animals in the area occurred human leptospirosis. Korean J Vet Res 1991 31: 209-215
Sieira R, Comerci DJ, Sanchez DO, Ugalde RA. A homologue of an operon required for DNA transfer in agrobacterium is required in Brucella abortus for virulence and intracellular multiplication. J Bacteriol 2000 182: 4849-4855
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