티트리에센셜 오일은 호주 원주민들의 전통적인 피부 소독제나 치료제로 널리 사용되어 왔으며, 항균효과와 주요성분 등 많은 보고가 있으나 추출 방법이나 사용 부위 등에 따라 효능의 차이를 보인다. 본 연구에서는 아로마테라피 등에 현재 많이 이용되고 있는 시판 티트리 오일의 성분과 효능을 평가하여, 다른 에센셜 오일과의 비교 이용을 용이하게 하고자 하였다. 티트리 오일의 주요성분은 GC-MS 분석에 의하여 ${\beta}$-terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%), limonene (4.65%)으로 밝혀졌으며, 5% 이하의 농도에서 3시간 미만까지는 피부세포에 독성이 없었다. 오일의 라디컬 소거능을 알아본 결과, DPPH와 ABTS의 양라디컬에 대하여 강한 소거능을 나타내어 강한 항산화능을 시사했다. 또한, 오일의 direct contact와 vapor-phase의 항균활성을 disc diffusion법으로 스크리닝 한 결과, direct contact 활성의 경우 그람음성균에 대하여 높은 활성을 나타내었으며, vapor는 S. aureus에 대하여 강한 효과를 나타내었다. 본 연구에서 실제 많이 사용되는 티트리 오일의 성분과 생물활성을 측정함으로써 허브 오일들의 정확한 선택과 활용을 위한 기본적인 결과를 얻었다.
티트리 에센셜 오일은 호주 원주민들의 전통적인 피부 소독제나 치료제로 널리 사용되어 왔으며, 항균효과와 주요성분 등 많은 보고가 있으나 추출 방법이나 사용 부위 등에 따라 효능의 차이를 보인다. 본 연구에서는 아로마테라피 등에 현재 많이 이용되고 있는 시판 티트리 오일의 성분과 효능을 평가하여, 다른 에센셜 오일과의 비교 이용을 용이하게 하고자 하였다. 티트리 오일의 주요성분은 GC-MS 분석에 의하여 ${\beta}$-terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%), limonene (4.65%)으로 밝혀졌으며, 5% 이하의 농도에서 3시간 미만까지는 피부세포에 독성이 없었다. 오일의 라디컬 소거능을 알아본 결과, DPPH와 ABTS의 양라디컬에 대하여 강한 소거능을 나타내어 강한 항산화능을 시사했다. 또한, 오일의 direct contact와 vapor-phase의 항균활성을 disc diffusion법으로 스크리닝 한 결과, direct contact 활성의 경우 그람음성균에 대하여 높은 활성을 나타내었으며, vapor는 S. aureus에 대하여 강한 효과를 나타내었다. 본 연구에서 실제 많이 사용되는 티트리 오일의 성분과 생물활성을 측정함으로써 허브 오일들의 정확한 선택과 활용을 위한 기본적인 결과를 얻었다.
The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-M...
The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-MS. ${\beta}$-Terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%) and limonene (4.65%) were the major components in the oil. The results tested by MTT assay indicated that the oil showed no cytotoxic effect, at concentrations up to 5%, for less than 3h. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction ($RC_{50}$) against ABTS radicals ($1.6{\pm}0.02%$) was slightly lower than DPPH radicals ($2.6{\pm}0.29%$). The direct contact and vapor-phase antibacterial activity of the oil were also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticus. All the Gram-negative bacterial strains tested showed more sensibility to the oil than the Gram-positive strains when compare to the effect of gentamycin. On the other hand, the vapor phase of the essential oil against S. aureus exhibited strongest inhibitory effect.
The essential oil of tea tree (Melaleuca alternifolia) is widely used in traditional Australian medicine for skin lesions and infected injuries. In the present study, we investigated the chemical composition, cytotoxicity and its biological activities. The composition of the oil was analyzed by GC-MS. ${\beta}$-Terpinene (20.87%), ${\alpha}$-pinene (17.60%), p-cymene (11.23%), 3-carene (10.40%), trans-anethole (8.47%) and limonene (4.65%) were the major components in the oil. The results tested by MTT assay indicated that the oil showed no cytotoxic effect, at concentrations up to 5%, for less than 3h. The antiradical capacity was evaluated by measuring the scavenging activity of the essential oil on the 2,20-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis 3-ethyl benzothiazoline-6-sulfonic acid (ABTS) radicals. The oil was able to reduce the both radicals dose-dependently, and the concentration required for 50% reduction ($RC_{50}$) against ABTS radicals ($1.6{\pm}0.02%$) was slightly lower than DPPH radicals ($2.6{\pm}0.29%$). The direct contact and vapor-phase antibacterial activity of the oil were also evaluated using disc diffusion method against Staphylococcus aureus, Streptococcus mutans, Listeria monocytogenes, Acinetobacter baumannii, Escherichia coli, and Vibrio parahaemolyticus. All the Gram-negative bacterial strains tested showed more sensibility to the oil than the Gram-positive strains when compare to the effect of gentamycin. On the other hand, the vapor phase of the essential oil against S. aureus exhibited strongest inhibitory effect.
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제안 방법
Free radical-scavenging capacity was evaluated by measuring the scavenging activity of the essential oil on DPPH and ABTS radicals. DPPH radical scavenging activities of the tea tree oils and of references at 5 μg/ml are presented in Fig.
Tea tree essential oil tested in this study was analyzed using GC-MS to identify its major components. Total ion chromatography of the oils revealed 24 significant peaks (Fig.
Total ion current (TIC) chromatograms were recorded in a mass range of 30-350 amu. The components were identified by comparison of their relative retention times with authentic substances and mass spectra with NIST, WILLY library data of the GC-MS system and literature data.
대상 데이터
is the absorbance of the control reaction (containing all reagents except the test sample), and A sample is the absorbance of the test sample. Tests were carried out in triplicate. For the final results, RC50 values (the concentrations required for 50% reduction of DPPH (0.
15 mM) at 30 min after starting the reaction) were calculated from the absorbance diminished by 50%. The experiment was performed in triplicate.
Trolox was used as reference synthetic antioxidant compound. The experiment was performed triplicate. Reduction of ABTS radical in percent (R%) and RC50 value were calculated the same as described in DPPH radical assay.
데이터처리
A significant difference from the respective controls for each experimental test condition was assessed using Student’s t test for each paired experiment.
성능/효과
Tests were carried out in triplicate. For the final results, RC50 values (the concentrations required for 50% reduction of DPPH (0.15 mM) at 30 min after starting the reaction) were calculated from the absorbance diminished by 50%. The experiment was performed in triplicate.
7 mm) even slightly higher than the contact phase. The inhibitory effects of the oil vapor phase showed 60.0, 66.7, 55.3, 68.8, 44.4% of the contact phase against A. baumannii, E. coil, V. parahaemolyticus, S. mutans and L. monocytogenes, respectively. Thus, the essential oil was most effective for inhibition of S.
후속연구
alterifolia contained low concentration of terpinen-4-ol in the early stage of seeding growth and its concentration rose by the time its mature. Our result is not consistent with these previous reports of tea tree oil, thus further analysis using various commercial tea tree oils and different origins are needed for validation of its major constituents.
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