[국내논문]RP-HPLC를 이용한 감초에서 liquiritin, glycyrrhizic acid, glabridin의 분리 Separation of liquiritin, glycyrrhizic acid and glabridin from licorice by RP-HPLC원문보기
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vo...
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
Reversed-phase high performance liquid chromatography (RP-HPLC) was used for the simultaneous determination of liquiritin (LQ), glycyrrhizic acid (GA) and glabridin from licorice. An optimized run condition was selected with a binary gradient elution of methanol-water which ramped 35/65 to 80/20 (vol. %) in 0.0-8.0 min and a flow rate of 1.0 mL/min. A good linearity was obtained between 0.2 mg/mL and 1.0 mg/mL for LQ and GA, and 0.01 mg/mL-0.2 mg/mL for glabridin with the relative standard deviations less than 0.90% (n=5). The developed method was successfully applied to determination of the three components from licorice samples. The mean recoveries of three components are 80.79% for liquiritin, 89.71% for glycyrrhizic acid and 72.50% for glabridin.
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문제 정의
Sun, et al1 However, method for the simultaneous separation of these three compounds was still not established. So the purpose of this study is to develop a new method to separate LQ, GA and glabridin from licorice simultaneously by RP-HPLC. Compared with the previously results, the present method is simple, rapid and could be used to simultaneously separate the three compounds simultanearsly from licorice.
제안 방법
The repeatability calculated as relative standard deviations (RSDs) were performed by injecting the standard solutions of LQ, GA and glabridin 5 times in one day and in 5 different days. The concentrations of standard solutions were 0.
In this experiment, a convenient method was developed for the simultaneous separation of LQ, GA and glabridin from licorice. Effect of the compositions of mobile phases, gradient profile and the concentrations of mobile phase additive were evaluated.
and glabridin from licorice. Effect of the compositions of mobile phases, gradient profile and the concentrations of mobile phase additive were evaluated. The developed method was successfully validated and applied to determine the three components in Chinese licorice sample.
대상 데이터
The HPLC system in this study is comprised of a M930 solvent deliveiy pump (Young Lin Co. Koiea), an UV detector (M 720 Absorbance Detector, YoungIn Scientific Co., Korea) and an integrated data system (Autochrowin. Ver. 1.42, Young Lin Co., Korea). The HPLC column (Ci8, 5 μm, 150x4.
이론/모형
The peak area values were plotted against the corresponding concentrations of the analytes and the calibration curves constructed by the means of leastsquare method. 0.
성능/효과
1% TFA in mobile phase was investigated. Compared the results of No.2 with No.5 and No. 4 with No. 6 in Table 2, the retention factors of LQ, GA obviously increased after using the TFA but the retention factor of glabridin was not changer obviously, so 0.1% TFA was obtained.
참고문헌 (16)
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T. Tanahashi, T. Mune, H. Morita, H. Tanahashi, Y. Isomura, T. Suwa, H. Daido, C. E. Gomez-Sancehz and K. Yasuda, J. Steroid Biochem. Molecular Biology, 80, 441-447(2002)
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