[논문]Effective Purification of Ginsenosides from Cultured Wild Ginseng Roots, Red Ginseng, and White Ginseng with Macroporous Resins

Li, Huayue; Lee, Jae-Hwa; Ha, Jong-Myung

doi:10.4014/jmb.0800.192

[국내논문] Effective Purification of Ginsenosides from Cultured Wild Ginseng Roots, Red Ginseng, and White Ginseng with Macroporous Resins 원문보기

Journal of microbiology and biotechnology, v.18 no.11, 2008년, pp.1789 - 1791

Li, Huayue (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) , Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University) , Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)

Abstract ▼ AI-Helper

This study was aimed (i) to develop an effective method for the purification of ginsenosides for industrial use and (ii) to compare the distribution of ginsenosides in cultured wild ginseng roots (adventitious root culture of Panax ginseng) with those of red ginseng (steamed ginseng) and white ginseng (air-dried ginseng). The crude extracts of cultured wild ginseng roots, red ginseng, and white ginseng were obtained by using a 75% ethanol extraction combined with ultrasonication. This was followed sequentially by AB-8 macroporous adsorption chromatography, Amberlite IRA 900 Cl anion-exchange chromatography, and Amberlite XAD16 adsorption chromatography for further purification. The contents of total ginsenosides were increased from 4.1%, 12.1%, and 11.3% in the crude extracts of cultured wild ginseng roots, red ginseng, and white ginseng to 79.4%, 71.7%, and 72.5% in the final products, respectively. HPLC analysis demonstrated that ginsenosides in cultured wild ginseng roots were distributed in a different ratio compared with red ginseng and white ginseng.

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제안 방법

Thus, a selective and high-yield purification method for ginsenosides needs to be developed. In this study, three different macroporous resins (AB-8, IRA 900 Cl, and XAD 16) were used to separate total ginsenosides Bpm cultured wild ginseng roots (CWG, adventitious root culture of Panax ginseng), red gmseng (RG steamed ginseng)and white ginseng (WQ air-dned ginseng). In addition, ten ginsenosides (Rbb Rb2, Rc, Rd, Re, Rf Rhf, Rg1, Rg2, and Rg3) (Fig.

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