In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorg...
In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.
In the present study, red ginseng extracts were fermented by Paecilomyces tenuipes and the protopanaxdiol-type ginsenosides in the extracts were bio-transformed to F2, Rg3, Rg5, Rk1, Rh2, and CK determined by a high-pressure liquid chromatography analysis. It indicates that P. tenuipes is a microorganism to biotransform protopanaxdiol-type ginsenosides to their less glucosidic metabolites. Other biotransformed metabolites during fermentation were also analyzed using a GC-MS and identified as 2-methyl-benzaldehyde, 4-vinyl-2-methylphenol, palmitic acid, and linoleic acid. Antiulcerogenic activity of the fermented red ginseng extract (FRGE) on gastric mucosal damage induced by 0.15 M HCl in ethanol in rats was evaluated. FRGE was shown to have a potent protective effect on gastritis with 60.5% of inhibition rate at the dose of 40 mg/kg when compared to 54.5% of the inhibition rate at the same dose for stillen, the currently used medicine for treating gastritis. Linoleic acid showed a strong inhibition on gastritis with 79.3% of inhibition rate at the dose of 40.0 mg/kg. FRGE exhibited a distinct anticancer activity including growth inhibition of the two human colon cancer cells HT29 and HCT116. HT29 cells were less susceptible to FRGE in comparison with HCT116 cells. Taken together, fungal fermentation of the red ginseng extract induced hydrolysis of some ginsenosides and FRGE exhibited potent antiulcerogenic and anticancer activities. These results refer to use FRGE as a new source for treating human diseases.
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가설 설정
a)The fermented red ginseng extract shows a significant difference when compared to the non-fermented red ginseng extract (p <0.05).
제안 방법
GC-MS analyses were performed using a GC-MS-QP 2010 spectrometer (Shimadzu, Japan), equipped with a 30 mm×0.25 mm (i.d.), 0.25 µm DM-5 capillary GC column (Dikma Technologies, China).
The purpose of this study was to enhance bioavailability of red ginseng extracts by a biotransformation process of ginsenosides using P. tenuipes and find some pharmacological activities. In the present study, biological activities of fermented red ginseng extract (FRGE) were determined in comparison to the nonfermented ginseng extracts (NFRGE).
대상 데이터
Male Sprague-Dawley rats (180–210 g) were used in the experiments.
데이터처리
The statistical significance was estimated using a student’s t-test.
성능/효과
Changes of ginsenoside constitution in FRGE are disclosed in Table 1 and Fig. 2. The total ginsenoside amounts in FRGE and NFRGE was 26.5 mg/g and 30.0 mg/g, respectively. In addition to the total amount of ginsenosides, the sum of two major constituents Rg1 and Rb1 was different in two samples as 10.
In conclusion, fungal biotransformation on red ginseng extract produced the protopanaxdiol-type metabolites and they referred antiulcerogenic activity on HCl-ethanol induced gastric mucosa damage in rats and anticancer activity on two human colon cancer cells HT-26 and HCT116. Therefore, fungal fermentation by P.
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