수온에 따른 유해성 Cochlodinium polykrikoides 적조생물의 세포생리 변화 Dependence of Sub-Cellular Activities of the Blooming and Harmful Dinoflagellate Cochlodinium Polykrikoides on Temperature원문보기
본 연구는 유해성 Cochlodinium polykrikoides 적조생물을 대상으로 수온변화에 따른 세포 생화학적 및 생리 활성도를 측정했다. Genomic DNA 함량은 $12^{\circ}C$ 및 $15^{\circ}C$에서 거의 비슷한 0.6을 보였으나, $18^{\circ}C$부터 급격히 높아져서 $24^{\circ}C$ 최고 1.8를 나타내었다. RNA와 total protein도 $24^{\circ}C$에 가장 높은 1.7과 0.07 ${\mu}g$$ml^{-1}$으로 나타났다. 광합성량도 수온에 따른 큰 변화를 보였다. 빛의 파장에 관계없이 $18^{\circ}C$ 이상에서 현저히 높은 값을 보였다. $24^{\circ}C$$ETR_{max}$ Ch1-Ch4까지의 범위는 537.9에서 602.5 ${\mu}mol$ electrons $g^{-1}$ Chl ${\alpha}s^{-1}$ 나타났다. Nitrate reductase와 ATPase 효소 활성도는 $24^{\circ}C$에서 각각 0.11 ${\mu}mol$$NO_{2}^{-}$${\mu}g^{-1}$ Chl ${\alpha}h^{-1}$ , 0.78 pmol 100 $mg^{-1}$ 나타났다. CHN 분석에서도 수온에 따라 C, H, N의 함량이 현저하게 상이했다. $27^{\circ}C$ 배양시 $24^{\circ}C$에 비하여 대부분의 세포생리물질이 낮게 보였다. 따라서 C. polykrikoides는 수온 변화에 대하여 세포대사물질의 함량이 많은 차이를 볼 수 있어서 초기 적조 발생 조건은 $18^{\circ}C$로 추측된다. 본 실험의 결과로 $24^{\circ}C$ 이상이 되면 C. polykrikoides 대번식은 세포 내 생리물질의 현저한 저하로 형성되기가 어려울 것으로 보인다.
본 연구는 유해성 Cochlodinium polykrikoides 적조생물을 대상으로 수온변화에 따른 세포 생화학적 및 생리 활성도를 측정했다. Genomic DNA 함량은 $12^{\circ}C$ 및 $15^{\circ}C$에서 거의 비슷한 0.6을 보였으나, $18^{\circ}C$부터 급격히 높아져서 $24^{\circ}C$ 최고 1.8를 나타내었다. RNA와 total protein도 $24^{\circ}C$에 가장 높은 1.7과 0.07 ${\mu}g$$ml^{-1}$으로 나타났다. 광합성량도 수온에 따른 큰 변화를 보였다. 빛의 파장에 관계없이 $18^{\circ}C$ 이상에서 현저히 높은 값을 보였다. $24^{\circ}C$$ETR_{max}$ Ch1-Ch4까지의 범위는 537.9에서 602.5 ${\mu}mol$ electrons $g^{-1}$ Chl ${\alpha}s^{-1}$ 나타났다. Nitrate reductase와 ATPase 효소 활성도는 $24^{\circ}C$에서 각각 0.11 ${\mu}mol$$NO_{2}^{-}$${\mu}g^{-1}$ Chl ${\alpha}h^{-1}$ , 0.78 pmol 100 $mg^{-1}$ 나타났다. CHN 분석에서도 수온에 따라 C, H, N의 함량이 현저하게 상이했다. $27^{\circ}C$ 배양시 $24^{\circ}C$에 비하여 대부분의 세포생리물질이 낮게 보였다. 따라서 C. polykrikoides는 수온 변화에 대하여 세포대사물질의 함량이 많은 차이를 볼 수 있어서 초기 적조 발생 조건은 $18^{\circ}C$로 추측된다. 본 실험의 결과로 $24^{\circ}C$ 이상이 되면 C. polykrikoides 대번식은 세포 내 생리물질의 현저한 저하로 형성되기가 어려울 것으로 보인다.
Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at $12^{\circ}C$ and $15^{\circ}C$. However, DNA s...
Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at $12^{\circ}C$ and $15^{\circ}C$. However, DNA significantly increased beyond $18^{\circ}C$ (p<0.05), to a maximum of 1.8 at $24^{\circ}C$. DNA concentration significantly decreased to 0.6. The concentrations of RNA and total protein were likely at their highest values of 1.7 and 0.07 ${\mu}g$$ml^{-1}$ at $24^{\circ}C$, respectively. RNA and total protein concentrations began to increase at $15^{\circ}C$. Oxygen availability between lower and higher temperatures was significantly different and increased from $18^{\circ}C$ according to light intensity, regardless of wavelengths (p<0.05). At $24^{\circ}C$, the highest value of the maximum electron transport rate ($ETR_{max}$), ranging from 537.9 (Ch 1) to 602.5 ${\mu}mol$ electrons $g^{-1}$ Chl ${\alpha}s^{-1}$ (Ch 4), was also apparent. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 ${\mu}mol$$NO_{2}^{-}$${\mu}g^{-1}$ Chl ${\alpha}h^{-1}$ and 0.78 pmol 100 $mg^{-1}$ at $24^{\circ}C$, respectively. In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). Most of the measurements for the cellular activities at $27^{\circ}C$, however, were less than at $24^{\circ}C$. These results suggest that the sub-cellular activities of C. polykrikoides are sensitive to changes in water temperature. It may be desirable to estimate at $18^{\circ}C$ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms beyond $24^{\circ}C$ because of a possibly significant decrease in molecular activity of C. polykrikoides.
Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at $12^{\circ}C$ and $15^{\circ}C$. However, DNA significantly increased beyond $18^{\circ}C$ (p<0.05), to a maximum of 1.8 at $24^{\circ}C$. DNA concentration significantly decreased to 0.6. The concentrations of RNA and total protein were likely at their highest values of 1.7 and 0.07 ${\mu}g$$ml^{-1}$ at $24^{\circ}C$, respectively. RNA and total protein concentrations began to increase at $15^{\circ}C$. Oxygen availability between lower and higher temperatures was significantly different and increased from $18^{\circ}C$ according to light intensity, regardless of wavelengths (p<0.05). At $24^{\circ}C$, the highest value of the maximum electron transport rate ($ETR_{max}$), ranging from 537.9 (Ch 1) to 602.5 ${\mu}mol$ electrons $g^{-1}$ Chl ${\alpha}s^{-1}$ (Ch 4), was also apparent. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 ${\mu}mol$$NO_{2}^{-}$${\mu}g^{-1}$ Chl ${\alpha}h^{-1}$ and 0.78 pmol 100 $mg^{-1}$ at $24^{\circ}C$, respectively. In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). Most of the measurements for the cellular activities at $27^{\circ}C$, however, were less than at $24^{\circ}C$. These results suggest that the sub-cellular activities of C. polykrikoides are sensitive to changes in water temperature. It may be desirable to estimate at $18^{\circ}C$ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms beyond $24^{\circ}C$ because of a possibly significant decrease in molecular activity of C. polykrikoides.
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문제 정의
polykrikoides associated with the fluc tuation of temperature. This study attempts to provide an analysis of the fluctuations of biochemical and molecular activities in C. polykrikoides in relation to main environ mental factors in the laboratory. Further, this study is an attempt to broaden the understanding of a cell biological magnitude to Cochlodinium blooms' process in nature.
제안 방법
polykrikoides was collected by centrifugation (250x g, 10 min) and preserved in -20°C until required. Genomic DNA was extracted from 0.05 g of wet weight of material using an AccuPrep Genomic DNA Extraction kit (Bioneer, Korea), Total RNA was determined by using a TRI Reagent kit (Molecular Research Center) after homogeniz ing the pellets. The RNA was frozen at -80°C until analyzed.
대상 데이터
pipette under a light microscope. The species was grown in f/2-Si medium [13] at 20°C under a light in tensity of 50 gmol m'2 s'1 from white fluorescent tubes (12L:12D light: dark cycle) and maintained in the South Sea Fisheries Research Institute.
This experiment consisted of quadruplicate cultures, each 1 1 of medium in 2 1 flask vessels. Each vessel was inoculated with approximately 500-700 cells ml'1 in a mul ti-chamber room (Dongyang Industry, Incheon, Korea).
데이터처리
Data analysis was carried out using a one-way analysis of variance (ANOVA) to observe the treatment effects, Duncans multiple range test by SPSS ver. 10.0 software was used to evaluate the significant differences between groups. Differences were considered statistically sig nificantly at p<0.
후속연구
polykrikoides in relation to main environ mental factors in the laboratory. Further, this study is an attempt to broaden the understanding of a cell biological magnitude to Cochlodinium blooms' process in nature.
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