알코올 급여 흰쥐에서 알코올성 간독성에 대한 누에배설물(silkworm excrement powder)의 영향을 검토하기 위하여 반합성 식이에 누에배설물을 3% (w/w) 수준으로 첨가하여 30일간 급여한 후 혈중 알코올 및 지질 농도, 간 기능 지표 효소 활성 및 간 조직 검사를 실시하였다. 임상생화학적으로 중요한 간 기능 지표 효소인 alanine aminotransferase (ALT), aspartate aminotransferase (AST), $\gamma$-glutamyl transpeptidase ($\gamma$-GTP) 및 lactate dehydrogenase (LDH) 활성이 알코올 대조군에서 증가하였다. 또한 혈중 알코올 농도도 알코올 섭취에 의해 증가하였다. 그러나 누에배설물 투여에 의해 혈중 ALT 및 LDH 활성은 현저하게 감소하였다. 혈중 중성지질, 콜레스테롤 농도는 알코올 대조군에 비해 누에배설물 투여에 의해 현저히 감소하였다. 또한 간 조직 검사에서 알코올 대조군에서 많은 지방적이 나타나 지방간이 확인되었으나 누에배설물 투여에 의해서는 지방적의 크기와 수가 많이 줄어드는 결과를 얻었다. 이상의 결과에서 누에배설물에 의한 알코올-유발 간독성의 개선효과는 간 조직의 임상생화학적 지표 효소의 활성 감소에 기인하는 것으로 나타났다.
알코올 급여 흰쥐에서 알코올성 간독성에 대한 누에배설물(silkworm excrement powder)의 영향을 검토하기 위하여 반합성 식이에 누에배설물을 3% (w/w) 수준으로 첨가하여 30일간 급여한 후 혈중 알코올 및 지질 농도, 간 기능 지표 효소 활성 및 간 조직 검사를 실시하였다. 임상생화학적으로 중요한 간 기능 지표 효소인 alanine aminotransferase (ALT), aspartate aminotransferase (AST), $\gamma$-glutamyl transpeptidase ($\gamma$-GTP) 및 lactate dehydrogenase (LDH) 활성이 알코올 대조군에서 증가하였다. 또한 혈중 알코올 농도도 알코올 섭취에 의해 증가하였다. 그러나 누에배설물 투여에 의해 혈중 ALT 및 LDH 활성은 현저하게 감소하였다. 혈중 중성지질, 콜레스테롤 농도는 알코올 대조군에 비해 누에배설물 투여에 의해 현저히 감소하였다. 또한 간 조직 검사에서 알코올 대조군에서 많은 지방적이 나타나 지방간이 확인되었으나 누에배설물 투여에 의해서는 지방적의 크기와 수가 많이 줄어드는 결과를 얻었다. 이상의 결과에서 누에배설물에 의한 알코올-유발 간독성의 개선효과는 간 조직의 임상생화학적 지표 효소의 활성 감소에 기인하는 것으로 나타났다.
The purpose of present study was to investigate the protective effect of silkworm excrement powder (SEP) on alcohol-induced hepatotoxicity in rats. Semisynthetic diet supplemented with SEP (3%, w/w) given to alcohol-feeding rats for 30 days, then blood and tissues were collected, processed and used ...
The purpose of present study was to investigate the protective effect of silkworm excrement powder (SEP) on alcohol-induced hepatotoxicity in rats. Semisynthetic diet supplemented with SEP (3%, w/w) given to alcohol-feeding rats for 30 days, then blood and tissues were collected, processed and used for alcohol concentration mensuration, various biochemical estimations and histopathological examination. Chronic alcohol administration resulted in significantly increase in the activities of the clinically important liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), $\gamma$-glutamyl transpeptidase ($\gamma$-GTP) and lactate dehydrogenase (LDH). Also, a highly significant increase in the blood alcohol level by alcohol treatment was observed. But alcohol-induced elevation of ALT and LDH levels markedly prevented and the level of blood alcohol decreased in SEP treated rats as compared to alcohol-administered control rats. SEP supplementation showed highly decreased the concentrations of total lipid, triglyceride and cholesterol in serum, as compared with alcohol treated control rats. Alcohol treatment induced the marked accumulation of large lipid droplets, hepatocytes necrosis and inflammation in the liver, but SEP administration attenuated to alcohol-induced accumulation of lipid droplets and hepatocyte necrosis. The results indicated that SEP may exert a protective effect against alcoholic hepatotoxicity through decreasing the activity of hepatic marker enzymes.
The purpose of present study was to investigate the protective effect of silkworm excrement powder (SEP) on alcohol-induced hepatotoxicity in rats. Semisynthetic diet supplemented with SEP (3%, w/w) given to alcohol-feeding rats for 30 days, then blood and tissues were collected, processed and used for alcohol concentration mensuration, various biochemical estimations and histopathological examination. Chronic alcohol administration resulted in significantly increase in the activities of the clinically important liver marker enzymes, alanine aminotransferase (ALT), aspartate aminotransferase (AST), $\gamma$-glutamyl transpeptidase ($\gamma$-GTP) and lactate dehydrogenase (LDH). Also, a highly significant increase in the blood alcohol level by alcohol treatment was observed. But alcohol-induced elevation of ALT and LDH levels markedly prevented and the level of blood alcohol decreased in SEP treated rats as compared to alcohol-administered control rats. SEP supplementation showed highly decreased the concentrations of total lipid, triglyceride and cholesterol in serum, as compared with alcohol treated control rats. Alcohol treatment induced the marked accumulation of large lipid droplets, hepatocytes necrosis and inflammation in the liver, but SEP administration attenuated to alcohol-induced accumulation of lipid droplets and hepatocyte necrosis. The results indicated that SEP may exert a protective effect against alcoholic hepatotoxicity through decreasing the activity of hepatic marker enzymes.
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문제 정의
We have hypothesized that the main efficacious ingredients of silkworm excrement, specifically the fiber and chlorophyll pigment compounds may exert hepatoprotectic and hypolipidemic effects when tested in experimental alcohol-induced liver damage in rats. Thus, this study seeks to investigate the possible protective effects of orally administrated SEP on acute alcohol-induced hepatotoxicity in rats.
This study was undertaken to examine the protective effect of SEP on hepatotoxicity of alcohol administered rats. Body and liver weights were measured as an indicator of alcoholic toxicity for this purpose.
가설 설정
1)Relative each tissue weight means the percent of the each tissue weight in the body weight.
제안 방법
(Daegu, Korea). Animal was housed individually in the suspended wire-mesh stainless steel cage under room temperature between 21 to 24℃ and lighting between 08:00 and 20:00. Animals were allowed to freely to semipurified basal diet for 1 week before the experiment.
Animals were allowed to freely to semipurified basal diet for 1 week before the experiment. Animals were then randomly divided into three experimental groups based on dietary categories: the normal rats fed with water, the alcohol feeding control rats fed with alcoholic beverage containing ethanol 30% (v/v), the SEP supplemented rats fed with alcohol and SEP (3%, w/w). The equal amount of SEP supplementation was replaced with cellulose into the alcohol feeding control rats (Table 1).
대상 데이터
Fresh silkworm excrement powder was obtained from the Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA, Suwon, Republic of Korea.
The chemically fixed sample was embedded in paraffin then sliced at an approximate 6 m thick for standard Hematoxylin & Eosin staining. The morphology of any lesions observed was classified and registered at the Anatomy Laboratory in the College of Medicine, Dong-A University, Busan, Republic of Korea.
데이터처리
The data from animal experiments are presented as the mean±SE, and were analyzed using one way analysis of variance (ANOVA), with the differences analyzed using the Duncan's new multiple-range test [6].
이론/모형
Hepatic histopathologic changes in alcohol treated rats (magnification ×200). Hepatocyte staining was carried out with the hematoxylin and eosin staining method.
참고문헌 (30)
Andersen, T. and C. Gluud. 1984. Liver morphology in morbid obesity: a literature study. Int. J. Obes. 8, 97-106.
Arimoto-Kobayashi, S., N. Inada, H. Nakano, H. Rai and H. Hayatsu. 1998. Iron-chlorophyllin-mediated conversion of 3-hydroxyamino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P -2(NHOH) into its nitroso derivative. Mutat. Res. 400, 259-269.
Cha, J. Y., Y. Mameda, K. Oogami, K. Yamamoto and T. Yanagita. 1998. Association between hepatic triacylglycerol accumulation induced by administering orotic acid and enhanced phosphatidate phosphohydrolase activity in rats. Biosci. Biotechnol. Biochem. 62, 508-513.
Cherng, J. Y. and M. S. Shim. 2006. Improving glycogenesis in streptozotocin (STZ) diabetic mice after administration of green algae Chlorella. Life Sci. 78, 1181-1186.
Gotoh, Y., S. Niimi, T. Hayakawa and T. Miyashita. 2004. Preparation of lactose-silk fibroin conjugates and their application as a scaffold for hepatocyte attachment. Biomaterials 25, 1131-1140.
Hwang, Y. K., H. J. Choi, M. Nam, J. D. Yoo and Y. H. Kim. 2006. Effects of Chlorella on metallothionein synthesis and binding capacity of cadmium poisoned rat liver and kidney. J. Exp. Biomed. Sci. 12, 23-27.
Hu, L. Q. and D. Y. Xu. 1989. HPLC separation and characterization of chlorin derivatives with intact ring V from acid degradation products of silkworm excrement crude chlorophyll mixture. Biomed. Chromatogr. 3, 72-74.
Inouye, K., M. Kurokawa, S. Nishikawa and M. Tsukada. 1998. Use of Bombyx mori fibroin as a substratum for cultivation of animal cells. J. Biochem. Biophys. Methods 37, 159-164.
Izu, H,, M. Shobayashi, Y. Manabe, K. Goto and H. Iefuji. 2006. Sake yeast suppresses acute alcohol-induced liver injury in mice. Biosci. Biotechnol. Biochem. 70, 2488-2493.
Kang, G. D., K. H. Lee, S. G. Do, C. S. Kim, J. G. Suh, Y. S. Oh and J. H. Nham. 2001. Effect of silk fibroin on the protection of alcoholic hepatotoxicity in the liver of alcohol preference mouse. Int. J. Indust. Entomol. 2, 15-18.
Kang, P. D., J. W. Kim, B. H. Sohn, K. Y. Kim, I. Y. Jung, M. J. Kim and K. S. Ryu. Accumulating pattern of $\alpha$ - glycosidase inhibitor in various silkworm varities. Korean J. Seric. Sci. 48, 25-27.
Kim, Y. H. 1998. Characteristics of greenish pigments from silkworm excrement by ethanol extraction. Korean J. Food Nutr. 44, 375-380.
Koivula, T. and M. Koivusalo. 1975. Different form of rat liver aldehyde dehydrogenase and their subcellular distribution. Biochim. Biophys. Acta. 397, 9-23.
Nagy, L. E. 2004. Molecular aspects of alcohol metabolism: transcription factors involved in early ethanol-induced liver injury. Annu. Rev. Nutr. 24, 55-78.
Ohtake, Y. and Y. Okumura. 1992. Establishing a high glutathione producing yeast species. Biosci. Ind. 50, 29-34.
Park, K. J., H. Y. Kim, B. J. Chang and H. H. Lee. 2004. Ameliorative effects of soy 11S protein on liver damage and hyperlipidemia in alcohol-fed rats. Biol. Pharm. Bull. 27, 1636-1641.
Park, K. J., M. J. Lee, H. Kang, K. S. Kim, S. H. Lee and I. Cho. 2002. Saeng-Maek-San, a medicinal herb complex, protects liver cell damage induced by alcohol. Biol. Pharm. Bull. 25, 1451-1455.
Seo, H. J., K. S. Jeong, M. K. Lee, Y. B. Park, U. J. Jung, H. J. Kim and M. S. Choi. 2003. Role of naringin supplement in regulation of lipid and ethanol metabolism in rats. Life Sci. 73, 933-946.
Shibata, S., K. Oda, N. Onodera-Masuoka, S. Matsubara, H. Kikuchi-Hayakawa, F. Ishikawa, A. Iwabuchi and H. Sansawa. 2001. Hypocholesterolemic effect of indigestible fraction of Chlorella vulgaris in cholesterol-fed rats. J. Nutr. Sci. Vitaminol. 47, 373-377.
Shon, M. Y., J. Y. Cha, C. H. Lee, S. H. Park and Y. S. Cho. 2007. Protective effect of administrated glutathioneenriched Saccharomyces cerevisiae FF-8 against carbon tetrachloride ( $CCl_4$ )-induced hepatotoxicity and oxidative stress in rats. Food Sci. Biotechnol. 16, 967-974.
Shim, J. Y., H. S. Shin, J. G. Han, H. S. Park, B. L. Lim, K. W. Chung and A. S. Om. 2008. Protective effects of Chlorella vulgaris on liver toxicity in cadmium-administered rats. J. Med. Food 11, 479-485.
Wang, W., S. Shen, Q. Chen, B. Tang, G. He, H. Ruan and U. N. Das. 2008. Hydrolyzates of silkworm pupae (Bombyx mori) protein is a new source of angiotensin-1 converting enzyme (ACE) inhibitory peptides (ACEIP). Current Pharm. Biotechnol. 9, 307-314.
Yin, M., K. Ikejima, G. E. Arteel, V. Seabra, B. U. Bradford, H. Kono, I. Rusyn and R. G. Thurman. 1998. Glycine accelerates recovery from alcohol-induced liver injury. J. Pharmacol. Exp. Ther. 286, 1014-1019.
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