[논문]천식 모델 생쥐에서 필발이 CD25+T 세포수, IgE, Histamine 생성량과 in vitro에서 Th1/Th2 Cytokine Balance에 미치는 영향

이영철; 김승형

doi:10.6116/kjh.2009.24.1.079

천식 모델 생쥐에서 필발이 CD25+T 세포수, IgE, Histamine 생성량과 in vitro에서 Th1/Th2 Cytokine Balance에 미치는 영향
Effects of Piperis Longi Fructus on Regulatory T Cells Number, IgE, Histamine Production in Asthma Model Mice and Th1/Th2 Cytokine Balance in vitro 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.24 no.1, 2009년, pp.79 - 88

이영철 (상지대학교 한의과대학 본초학교실) , 김승형 (대전대학교 동서생명과학연구원)

Abstract ▼ AI-Helper

Objectives : It has been recently shown that Piperis Longi Fructus (PLF) is involved in the reduction of eosinophil recruitment and production of Th2 cytokines in vivo. However, the main therapeutic mechanisms of PLF remains a matter of considerable debate. To investigate the therapeutic mechanisms of PLF, we examined the influence of PLF on regulatory T cells number, IgE, histamine production in vivo and Th1/Th2 cytokine balance in vitro. Methods : All mice were immunized on two different days (21 days and 7 days before inhalational exposure) by i.p. injections of 0.2 $m\ell$ alum-precipitated Ag containing 100 ${\mu}g$ of OVA bound to 4 mg of aluminum hydroxide in PBS. Seven days after the second sensitization, mice were exposed to aerosolized ovalbumin for 30 min/day on 3 days/week for 12 weeks(at a flow rate of 250 L/min, 2.5％ ovalbumin in normal saline) and PLF (150 mg/kg) were orally administered 3 times a week for 8 weeks. Splenocytes from C57BL/6 mice at 8 weeks of age were stimulated with anti-CD3 (1 mg/ml) plus anti-CD28 (1 mg/ml) antibody for 48hrs. IL-4 and IFN-$\gamma$ in the culture supernatants were measured by ELISA Results : The suppressive effects of PLF on asthma model were demonstrated by the increase the number of regulatory T cells and by reducing IgE, histamine production in vivo and modulation of Th1/Th2 cytokine balance. Conclusions : These results indicate that PLF has a deep inhibitory effects on asthma model mice by increase the number of regulatory T cells, and by reducing IgE, histamine production.

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제안 방법

To investigate the therapeutic mechanisms of PLF, we examined the influence of PLF on regulatory T cells number, IgE, histamine production in vivo and Th1/Th2 cytokine balance in vitro. In this study, we focused on the immunoregulatory effects of PLF on the regulatory T cells and Th1/Th2 balance in ovalbumin (OVA)-induced asthma model mice.
To investigate the effects of PLF on IgE synthesis and histamine release, PLF (150 mg/kg) were orally administered 3 times a week for 8 weeks. PLF prevents IgE production in both BALF and serum, but not histamine release.
However, the main therapeutic mechanisms of PLF remains a matter of considerable debate. To investigate the therapeutic mechanisms of PLF, we examined the influence of PLF on regulatory T cells number, IgE, histamine production in vivo and Th1/Th2 cytokine balance in vitro. In this study, we focused on the immunoregulatory effects of PLF on the regulatory T cells and Th1/Th2 balance in ovalbumin (OVA)-induced asthma model mice.
To study whether PLF were related to Th1/Th2 cytokine balance,after 48 hrs of culture, supernatant from the splenocytes of PLF treated group were analyzed the levels of IL-4 and IFN-γ in each samples.

대상 데이터

C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (three times a week) for asthma induction. Two experimental groups were treated with different concentrations of PLF for the later 8 weeks (3 times/week).

데이터처리

All data represent the standard deviation of at least three different determinants and were compared using Student's unpaired t-test.
For statistical analysis of data, P-values were analyzed using a unpaired Student t-test software program (Startview 5.1; Abacus Concepts, Berkeley, CA). Results were considered statistically significant if p values were < 0.

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