[논문]Curcuma longae Radix, Phellinus linteus 및 Scutellariae Radix 혼합추출물의 <tex>$A{\\beta}$</tex> (25-35) 유도 배양신경세포독성 및 마우스기억손상 억제효과

김주연; 정하연; 반주연; 유재국; 배기환; 성연희

Curcuma longae Radix, Phellinus linteus 및 Scutellariae Radix 혼합추출물의 $A{\\beta}$ (25-35) 유도 배양신경세포독성 및 마우스기억손상 억제효과
Ethanol Extract of Three Plants of Curcuma longae Radix, Phellinus linteus, and Scutellariae Radix Inhibits Amyloid $\\beta$ Protein (25-35)-Induced Neurotoxicity in Cultured Neurons and Memory Impairment in Mice 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.17 no.6, 2009년, pp.388 - 396

김주연 (충북대학교수의과대학) , 정하연 (충북대학교수의과대학) , 반주연 (단국대학교치과대학) , 유재국 (한국신약) , 배기환 (충남대학교약학대학) , 성연희 (충북대학교수의과대학)

Abstract ▼ AI-Helper

The present study investigated an ethanol extract (HS0608) of a mixture of three medicinal plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix for possible neuroprotective effects on neurotoxicity induced by amyloid $\beta$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $10\;{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-50\;{\mu}g/m{\ell}$, HS0608 inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in primary cultures of rat cortical neurons. Memory loss induced by intracerebroventricular injection of ICR mice with 15 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with HS0608 (25, 50 and 100 mg/kg, p.o. for 7 days) as measured by a passive avoidance test. From these results, we suggest that the antidementia effect of HS0608 is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that HS0608 may have a therapeutic role in preventing the progression of Alzheimer's disease.

주제어

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가설 설정

, 2005; Pitchumoni and Doraiswamy, 1998), free radical scavengers and anti-inflammatory agents have attracted considerable attention as potential neuroprotective agents. We hypothesized that the combination of three plants of Curcuma longae radix, Phellinus linteus, and Scutellariae radix might protect neurons against neurodegenerative diseases such as AD and ischemia on the basis of their antioxidant, anti-inflammatory, and neuroprotective activities. The aim of the present study was to determine whether an ethanol extract of a mixture of three herbs of Curcuma longae radix, Phellinus linteus, and Scutellariae radix, which was named as HS0608, has a protective effect against Aβ (25-35)-induced memory deficits in mice and Aβ (25-35)-induced neuronal damage in cultured rat cortical neurons.

제안 방법

The aim of the present study was to determine whether an ethanol extract of a mixture of three herbs of Curcuma longae radix, Phellinus linteus, and Scutellariae radix, which was named as HS0608, has a protective effect against Aβ (25-35)-induced memory deficits in mice and Aβ (25-35)-induced neuronal damage in cultured rat cortical neurons.
To test for any possible effect of Aβ (25-35) on motor function, the locomotor activity of the animals was measured using a photobeam monitoring system (AM1051, Benwick Electronics, Benwick, UK), and motor coordination was measured using a rota-rod apparatus (Daejong Inc., Seoul, Korea).

대상 데이터

In conclusion, the protection against Aβ (25-35)-induced neuronal cell damage in culture and Aβ (25-35)-induced memory deficit in vivo may explain the inhibitory action of HS0608 on the progression of AD. Further studies should determine the specific components in three plants of HS0608 that are responsible for preventing the memory impairment.
The dried Curcuma longae radix and Scutellariae radix were purchased at Kyungdong Folk Medicine market, Seoul, Korea, and fruiting bodies of cultured Phellinus linteus were supplied from Han Kook Shin Yak, Nonsan, Chungnam. These plants were identified by one of the authors, Dr.

데이터처리

Data are expressed as mean±SEM and statistical significance was assessed by one-way analysis of variance (ANOVA) and Tukey’s tests.

이론/모형

Inhibitory effect of HS0608 on Aβ (25-35)-induced neuronal cell death in cultured cortical neurons. Neuronal cell death was measured using the MTT assay. The MTT absorbance from untreated cells was normalized to 100%.

성능/효과

In conclusion, the protection against Aβ (25-35)-induced neuronal cell damage in culture and Aβ (25-35)-induced memory deficit in vivo may explain the inhibitory action of HS0608 on the progression of AD. Further studies should determine the specific components in three plants of HS0608 that are responsible for preventing the memory impairment.
These results indicate that HS0608 might prevent Aβ (25-35)–induced Ca2+ entry through VDCC- and/ or NMDA-receptor–coupled channels to inhibit ROS generation and then neuronal death, although the mechanism by which HS0608 blocks the channels is not clear.

후속연구

, 2006), repectively, suggesting that inhibition of Aβ (25-35)–induced neuronal death by HS0608 might be due to their ROS scavenging activity. Further study to elucidate the precise mechanism should be performed.

참고문헌 (66)

Adaramoye OA, Adesanoye OA, Olusola A and Akinloye O. (2002). Antioxidant activity of turmeric extracts (Curcuma longa L.) and its effect on iron/ascorbate induced lipid peroxidation. Biokemistri. 12:127-135
Ajith TA and Janardhanan KK. (2002). Antioxidant and antihepatotoxic activities of Phellinus rimosus (Berk) Pilat. Journal of Ethnopharmacology. 81:387-391

상세보기
Alkam T, Nitta A, Mizoguchi H, Itoh A and Nabeshima T. (2007). A natural scavenger of peroxynitrites, rosmarinic acid, protects against impairment of memory induced by Abeta (25-35). Behavioral Brain Research. 180:139-145

상세보기
Atack JR, Perry EK, Bonham JR, Perry RH, Tomlinson BE, Blessed G and Fairbairn A. (1983). Molecular forms of acetylcholinesterase in senile dementia of Alzheimer type: selective loss of the intermediate (10S) form. Neuroscience Letters. 40:199-204

상세보기
Ban JY, Jeon SY, Bae K, Song KS and Seong YH. (2006). Catechin and epicatechin from Smilacis chinae rhizome protect cultured rat cortical neurons against amyloid beta protein (25-35)-induced neurotoxicity through inhibition of cytosolic calcium elevation. Life Sciences. 79:2251-2259

상세보기
Ban JY and Seong YH. (2005). Blockade of 5-HT3 receptor with MDL72222 and Y25130 reduces $\beta$ -amyloid protein (25-35)-induced neurotoxicity in cultured rat cortical neurons. European Journal of Pharmacology. 520:12-21

상세보기
Bensky D, Gamble A and Kaptchuk T. (1992). Chinese Herbal Medicine Materia Medica. Eastland Press, Seattle, WA. pp.107-109
Butterfield DA, Drake J, Pocernich C and Castegna A. (2001). Evidence of oxidative damage in Alzheimer's disease brain: central role for amyloid beta-peptide. Trends in Molecular Medicine. 7:548-554

상세보기
Butterfield DA and Lauderback CM. (2002). Lipid peroxidation and protein oxidation in Alzheimer's disease brain: potential causes and consequences involving amyloid beta-peptideassociated free radical oxidative stress. Free Radical Biology and Medicine. 32:1050-1060

상세보기
Butterfield DA, Reed T, Newman SF and Sultana R. (2007). Roles of amyloid beta-peptide-associated oxidative stress and brain protein modifications in the pathogenesis of Alzheimer's disease and mild cognitive impairment. Free Radical Biology and Medicine. 43:658-677

상세보기
Chan PH. (2001). Reactive oxygen radicals in signaling and damage in the ischemic brain. Journal of Cerebral Blood Flow and Metabolism. 21:2-14

상세보기
Chang HY, Sheu MJ, Yang CH, Lu TC, Chang YS, Peng WH, Huang SS and Huang GJ. Analgesic effects and the mechanisms of anti-inflammation of hispolon in mice. Evidence-Based Complementary and Alternative Medicine. eCAM Advance Access published April 6, 2009. 1-8
Chen CY, Jang JH, Park MH, Hwang SJ, Surh YJ and Park OJ. (2007). Attenuation of Abeta-induced apoptosis of plant extract (Saengshik) mediated by the inhibition of mitochondrial dysfunction and antioxidative effect. Annals of the New York Academy of Sciences.1095:399-411

상세보기
Cho SO, Ban JY, Kim JY, Jeong HY, LiS, Song KS, Bae K and Seong YH. (2009). Aralia cordata protects against amyloid β protein (25-35)-induced neurotoxicity in cultured neurons and has antidementia activities in mice. Journal of Pharmacological Sciences. 111:22-33

상세보기
Dairam A, Limson JL, Watkins GM, Antunes E and Daya S. (2007). Curcuminoids, curcumin, and demethoxycurcumin reduce lead-induced memory deficits in male Wistar rats. Journal of Agricultural Food Chemistry. 55:1039-1044

상세보기
Ekinci FJ, Linsley MD and Shea TB. (2000). Beta-amyloidinduced calcium influx induces apoptosis in culture by oxidative stress rather than tau phosphorylation. Molecular Brain Research. 76:389-395

상세보기
Ekinci FJ, Malik KU and Shea TB. (1999). Activation of the L voltage-sensitive calcium channel by mitogen-activated protein (MAP) kinase following exposure of neuronal cells to betaamyloid. MAP kinase mediates beta-amyloid-induced neurodegeneration. Journal of Biological Chemistry. 274:30322-30327

상세보기
Frautschy SA, Hu W, Kim P, Miller SA, Chu T, Harris-White ME and Cole GM. (2001). Phenolic anti-inflammatory antioxidant reversal of Abeta-induced cognitive deficits and neuropathology. Neurobiology of Aging. 22:993-1005

상세보기
Gitter BD, Cox LM, Rydel RE and May PC. (1995). Amyloid beta peptide potentiates cytokine secretion by interleukin-1 beta-activated human astrocytoma cells. Proceedings of the National Academy of Sciences U S A. 92:10738-10741

상세보기
Gray CW and Patel AJ. (1995). Neurodegeneration mediated by glutamate and beta-amyloid peptide: a comparison and possible interaction. Brain Research. 691:169-179
Guo LY, Cai XF, Lee JJ, Kang SS, Shin EM, Zhou HY, Jung JW and Kim YS. (2008). Comparison of suppressive effects of demethoxycurcumin and bisdemethoxycurcumin on expressions of inflammatory mediators in vitro and in vivo. Archives Pharmacal Research. 31:490-496

원문보기 상세보기
Heo H, Shin Y, Cho W, Choi Y, Kim H and Kwon YK. (2009). Memory improvement in ibotenic acid induced model rats by extracts of Scutellaria baicalensis. Journal of Ethnopharmacology. 122:20-27

상세보기
Hoehn BD, Palmer TD and Steinberg GK. (2005). Neurogenesis in rats after focal cerebral ischemia is enhanced by indomethacin. Stroke. 36:2718-2724

상세보기
Huang KC. (1999). The Pharmacology of Chinese Herbs. CRC press, Boca Raton, FL. pp.385
Ivins KJ, Ivins JK, Sharp JP and Cotman CW. (1999). Multiple pathways of apoptosis in PC12 cells. CrmA inhibits apoptosis induced by beta-amyloid. Journal of Biological Chemistry. 274:2107-2112

상세보기
Jang JH and Surh YJ. (2005). Beta-amyloid-induced apoptosis is associated with cyclooxygenase-2 up-regulation via the mitogen-activated protein kinase-NF-kappaB signaling pathway. Free Radical Biology and Medicine 38:1604-1613

상세보기
Kapoor LD. (1990). Handbook of Ayurvedic Medicinal plants. CRC Press, Boca Raton. pp.149-150
Kim JY, Ju HS, Ban JY, Song KS and Seong YH. (2008). Moutan cortex extract inhibits amyloid β protein (25-35)-induced neurotoxicity in cultured rat cortical neurons. Korean Journal of Medicinal Crop Sciences. 16:409-415
Kim HG, Yoon DH, Lee WH, Han SK, Shrestha B, Kim CH, Lim MH, Chang W, Lim S, Choi S, Song WO, Sung JM, Hwang KC and Kim TW. (2007). Phellinus linteus inhibits inflammatory mediators by suppressing redox-based NF-kappaB

상세보기
Koh JY, Yang LL and Cotman CW. (1990). Beta-amyloid protein increases the vulnerability of cultured cortical neurons to excitotoxic damage. Brain Research. 533:315-320

상세보기
Kontush A. (2001). Amyloid-beta: an antioxidant that becomes a pro-oxidant and critically contributes to Alzheimer's disease. Free Radical Biology and Medicine. 31:1120-1131

상세보기
Lee BY, Ban JY and Seong YH. (2005). Chronic stimulation of GABAA receptor with muscimol reduces amyloid beta protein (25-35)-induced neurotoxicity in cultured rat cortical cells. Neuroscience Research. 52:347-356

상세보기
Lee SB, Kim JY, Cho SO, Ban JY, Ju HS, Bae K and Seong YH. (2007). Extract of Cedrela sinensis leaves protects neuronal cell damage induced by hydrogen peroxide in cultured rat neurons. Korean Journal of Medicinal Crop Scidence. 15:444-450

원문보기 상세보기
Maurice T, Lockhart BP and Privat A. (1996). Amnesia induced in mice by centrally administered beta-amyloid peptides involves cholinergic dysfunction. Brain Research. 706:181-193

상세보기
McDonald DR, Brunden KR and Landreth GE. (1997). Amyloid fibrils activate tyrosine kinase-dependent signaling and superoxide production in microglia. Journal of Neuroscience. 17:2284-2294

상세보기
Miranda S, Opazo C, Larrondo LF, Munoz FJ, Ruiz F, Leighton F and Inestrosa NC. (2000). The role of oxidative stress in the toxicity induced by amyloid beta-peptide in Alzheimer's disease. Progress in Neurobiology. 62:633-648

상세보기
Morais Cardoso S, Swerdlow RH and Oliveira CR. (2002). Induction of cytochrome c-mediated apoptosis by amyloid beta 25-35 requires functional mitochondria. Brain Research. 931:117-125

상세보기
Mu X, He G, Cheng Y, Li X, Xu B and Du G. (2009). Baicalein exerts neuroprotective effects in 6-hydroxydopamine-induced experimental parkinsonism in vivo and in vitro. Pharmacology, Biochemistry and Behavior. 92:642-648

상세보기
Nitta A, Fukuta T, Hasegawa T and Nabeshima T. (1997). Continuous infusion of beta-amyloid protein into the rat cerebral ventricle induces learning impairment and neuronal and morphological degeneration. Japanese Journal of Pharmacology. 73:51-57

상세보기
Pike CJ, Walencewicz-Wasserman AJ, Kosmoski J, Cribbs DH, Glabe CG and Cotman CW. (1995). Structure-activity analyses of beta-amyloid peptides: contributions of the beta 25-35 region to aggregation and neurotoxicity. Journal of Neurochemistry. 64:253-265

상세보기
Pitchumoni SS and Doraiswamy PM. (1998). Current status of antioxidant therapy for Alzheimer's Disease. Journal of the American Geriatrics Society. 46:1566-1572

상세보기
Rajakrishnan V, Viswanathan P, Rajasekharan KN and Menon VP. (1999). Neuroprotective role of curcumin from curcuma longa on ethanol-induced brain damage. Phytotherapy Research. 13:571-574

상세보기
Richardson JS, Zhou Y and Kumar U. (1996). Free radicals in the neurotoxic actions of beta-amyloid. Annals of the New York Academy of Sciences. 777:362-367

상세보기
Rogers J, Kirby LC, Hempelman SR, Berry DL, McGeer PL, Kaszniak AW, Zalinski J, Cofield M, Mansukhani L, Willson P et al. (1993). Clinical trial of indomethacin in Alzheimer's disease. Neurology. 43:1609-1611

상세보기
Sano M, Ernesto C, Thomas RG, Klauber MR, Schafer K, Grundman M, Woodbury P, Growdon J, Cotman CW, Pfeiffer E, Schneider LS and Thal LJ. (1997) A controlled trial of selegiline, alpha-tocopherol, or both as treatment for

상세보기
Sharma S, Kulkarni SK and Chopra K. (2006). Curcumin, the active principle of turmeric (Curcuma longa), ameliorates diabetic nephropathy in rats. Clinical and Experimental Pharmacology and Physiology. 33:940-945

상세보기
Shukla PK, Khanna VK, Ali MM, Khan MY and Srimal RC. (2008). Anti-ischemic effect of curcumin in rat brain. Neurochemical Research. 33:1036-1043

상세보기
Sliva D, Jedinak A, Kawasaki J, Harvey K and Slivova V. (2008). Phellinus linteus suppresses growth, angiogenesis and invasive behaviour of breast cancer cells through the inhibition of AKT signalling. British Journal of Cancer. 98:1348-1356

상세보기
Smith MA, Richey Harris PL, Sayre LM, Beckman JS and Perry G. (1997). Widespread peroxynitrite-mediated damage in Alzheimer's disease. Journal of Neuroscience. 17:2653-2657

상세보기
Solntseva EI, Bukanova JV, Marchenko E and Skrebitsky VG. (2007). Donepezil is a strong antagonist of voltage-gated calcium and potassium channels in molluscan neurons. Comparative Biochemistry and Physiology. 144:319-326

상세보기
Srinivasan KR. (1953) A chromatographic study of the curcuminoids in Curcuma longa, L. Journal of Pharmacy and Pharmacology. 5:448-457

상세보기
Srivastava KC, Bordia A and Verma SK. (1995). Curcumin, a major component of food spice turmeric (Curcuma longa) inhibits aggregation and alters eicosanoid metabolism in human blood platelets. Prostaglandins Leukotriens and Essential Fatty Acids. 52:223-227

상세보기
Stepanichev MY, Moiseeva YV, Lazareva NA, Onufriev MV and Gulyaeva NV. (2003). Single intracerebroventricular administration of amyloid-beta (25-35) peptide induces impairment in short-term rather than long-term memory in rats. Brain Research Bulletin. 61:197-205

상세보기
Su S, He CM, Li LC, Chen JK and Zhou TS. (2008). Genetic characterization and phytochemical analysis of wild and cultivated populations of Scutellaria baicalensis. Chemistry and Biodiversity. 5:1353-1363

상세보기
Suzuki S, Kawamata T, Okada Y, Kobayashi T, Nakamura T and Hori T. Filtrate of Phellinus linteus Broth Culture Reduces Infarct Size Significantly in a Rat Model of Permanent Focal Cerebral Ischemia. Evidence-Based Complementary and Alternative Medicine. eCAM Advance Access published January 20, 2009. 1-7
Ueda K, Shinohara S, Yagami T, Asakura K and Kawasaki K. (1997). Amyloid beta protein potentiates $Ca^{2+}$ influx through Ltype voltage-sensitive $Ca^{2+}$ channels: a possible involvement of free radicals. Journal of Neurochemistry. 68:265-271

상세보기
Um MY, Choi WH, Aan JY, Kim SR and Ha TY. (2006). Protective effect of Polygonum multiflorum Thunb on amyloid beta-peptide 25-35 induced cognitive deficits in mice. Journal of Ethnopharmacology. 104:144-148

상세보기
Van Dam D and De Deyn PP. (2006). Drug discovery in dementia: the role of rodent models. Nature Reviews Drug Discovery. 5:956-970

상세보기
Wang Q, Sun AY, Simonyi A, Jensen MD, Shelat PB, Rottinghaus GE, MacDonald RS, Miller DK, Lubahn DE, Weisman GA and Sun GY. (2005). Neuroprotective mechanisms of curcumin against cerebral ischemia-induced

상세보기
Wang SY, Wang HH, Chi CW, Chen CF and Liao JF. (2004). Effects of baicalein on beta-amyloid peptide-(25-35)-induced amnesia in mice. European Journal of Pharmacology. 506:55-61

상세보기
Wenk GL. (2006). Neuropathologic changes in Alzheimer's disease: potential targets for treatment. Journal of Clinical Psychiatry. 67 Suppl 3:3-7; quiz 23
Yamada K, Tanaka T, Han D, Senzaki K, Kameyama T and Nabeshima T. (1999). Protective effects of idebenone and alpha-tocopherol on beta-amyloid-(1-42)-induced learning and memory deficits in rats: implication of oxidative stress in betaamyloid-induced neurotoxicity in vivo. European Journal of Neuroscience. 11:83-90

상세보기
Yan XZ, Qiao JT, Dou Y and Qiao ZD. (1999). Beta-amyloid peptide fragment 31-35 induces apoptosis in cultured cortical neurons. Neuroscience. 92:177-184

상세보기
Yu ZF, Kong LD and Chen Y. (2002). Antidepressant activity of aqueous extracts of Curcuma longa in mice. Journal of Ethnopharmacology. 83:161-165

상세보기
Yune TY, Lee JY, Cui CM, Kim HC and Oh TH. (2009). Neuroprotective effect of Scutellaria baicalensis on spinal cord injury in rats. Journal of Neurochemistry. 110:1276-1287

상세보기
Zhang Y, Wang X, Xu Z, Liu Z, Ni Q, Chu X, Qiu M, Zhao A and Jia W. (2006). Protective effect of flavonoids from Scutellaria baicalensis Georgi on cerebral ischemia injury. Journal of Ethnopharmacology. 108:355-360

상세보기

저자의 다른 논문 :

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