[논문]머루전초, 독활전초, 감초 혼합추출물의 Amyloid β Protein (25-35) 유발 신경 독성에 대한 억제효과

장지연; 성연희

doi:10.7783/kjmcs.2014.22.2.105

머루전초, 독활전초, 감초 혼합추출물의 Amyloid β Protein (25-35) 유발 신경 독성에 대한 억제효과
Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.22 no.2, 2014년, pp.105 - 112

Abstract ▼ AI-Helper

The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced by Amyloid ${\beta}$ protein ($A{\beta}$) (25-35) in cultured rat cortical neurons and antidementia activity in mice. Exposure of cultured cortical neurons to $15{\mu}M$ $A{\beta}$ (25-35) for 36 h induced neuronal apoptotic death. At $1-30{\mu}g/m{\ell}$, SSB inhibited neuronal death, elevation of intracellular calcium concentration ($[Ca^{2+}]_i$), and generation of reactive oxygen species (ROS) induced by $A{\beta}$ (25-35) in cultured cortical neurons. Memory impairment and increase of acetylcholinesterase activity induced by intracerebroventricular injection of mice with 16 nmol $A{\beta}$ (25-35) was inhibited by chronic treatment with SSB (25, 50 and 100 mg/kg, p.o., for 8 days). From these results, it is suggested that antidementia effect of SSB is due to its neuroprotective effect against $A{\beta}$ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression of Alzheimer's disease.

주제어

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가설 설정

, 2012). Therefore, we hypothetized that mixture of three plants might show more effective and synergistic neuroprotective effect with the reduction of possible individual toxicity due to large quantity.

대상 데이터

The leaves and stems of V. amurensis and the aerial parts of A. cordata were collected at Keryong Mountain in Daejeon, Korea, from May to July and Glycyrrhizae radix (Korean) was purchased from Daegu Oriental Pharm Co. at Daegu, Korea. These plants were identified by Dr.

데이터처리

Data were expressed as mean ± SEM and statistical significance was assessed by one-way analysis of variance (ANOVA) and Tukey’s tests.

이론/모형

Retention trial was given 24 h after the acquisition trial. Cholinesterase activity of mice whole brain isolated immediately after the retention trial of passive avoidance test was determined using the spectrophotometric method (Ellman et al., 1961).
Inhibitory effect of SSB on Aβ (25-35)-induced neuronal cell death in cultured cortical neurons. Neuronal cell death was measured using the MTT assay. The MTT absorbance from untreated cells was normalized to 100% as a control.

성능/효과

In conclusion, it is evident that SSB could provide a marked protection against Aβ (25-35)-induced neuronal cell damage in vitro and Aâ (25-35)-induced memory deficit in vivo. These may explain the inhibitory action of SSB on the progression of AD.
Mixture of three plants, SSB, showed a maximal inhibition of Aβ (25-35)-induced memory impairment by a dosage of 100 ㎎/㎏ in the present study. These results indicate that three medicinal plants acted synergistically to produce neuroprotection, since maximal effect of SSB was shown at much lower concentration than the sum of maximal effect concentration of each plant.

후속연구

, 2007), respectively, suggesting that inhibition of Aβ (25-35)-induced neuronal death by SSB might be due to their ROS scavenging activity. Further study should be conducted to elucidate the precise mechanism.

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