[논문]Analysis of Residual Furan in Human Blood Using Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS)

Lee, Yun-Kyung; Jung, Seung-Won; Lee, Sung-Joon; Lee, Kwang-Geun

[국내논문] Analysis of Residual Furan in Human Blood Using Solid Phase Microextraction-Gas Chromatography/Mass Spectrometry (SPME-GC/MS) 원문보기

Food science and biotechnology, v.18 no.2, 2009년, pp.379 - 383

Lee, Yun-Kyung (Department of Food Science and Technology, Dongguk University) , Jung, Seung-Won (Department of Food Science and Technology, Dongguk University) , Lee, Sung-Joon (Division of Food Science, College of Life Science and Biotechnology, Institute of Biomedical Sciences and Safety, Korea University) , Lee, Kwang-Geun (Department of Food Science and Technology, Dongguk University)

Abstract ▼ AI-Helper

For an accurate risk assessment of furan, a potential human carcinogen, levels must be determined in human blood plasma using a simple and robust assay. In this study, solid phase microextraction-gas chromatography/mass spectrometry (SPME-GC/MS) was used to analyze blood plasma levels of furan in 100 healthy individuals who consumed a normal diet. The subjects were 30 to 70 years of age and 51% were women. Ultimately, an analytical method was established for analyzing furan in human blood. The limit of quantification (LOQ) and furan recovery rate in blood were 1.0 ppb and 104%, respectively. Finally, furan was detected in 21 individuals (13 males, 8 females) with levels ranging up to 17.86 ppb (ng furan/g food).

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제안 방법

SPME integrates sampling, extraction, pre-concentration, and sample introduction into a single process, resulting in high sample throughput (15). Therefore, we chose the SPME-GC/MS method to analyze furan in this study.
For accurate risk assessment in humans, simple and robust assays should be used to determine blood furan levels. In this study, SPME-GC/MS was employed and then validated as an analytical method for determining residual furan levels in human blood plasma.
The following GC oven temperature program was applied: 50℃ for 5 min, 25℃/min to 230℃, and 230℃ for 2 min. The mass spectrometer was operated in selectiveion monitoring mode (SIM) by recording the currents of the following ions: m/z 68 and 39 for furan, and m/z 72 and 42 for d₄-furan. The corresponding ion ratios for furan and d₄-furan were determined for each measurement.
In this study, a simple method for determining furan levels in human blood was developed and validated. The SPME-GC/MS system was modified from the method of Goldmann et al.
To validate the procedure, we performed a recovery test by adding 3.2 ppb of furan and 1.6 ppb of internal standard (d4-furan) to blood samples without furan, as described in the materials and methods. The recovery rate of the blood samples was 104.
According to the results of this study, the tested analytical method would be robust and accurate in determining human blood levels of furan. The data presented in this study would be helpful in the risk assessment process by describing a method to quantify a potential biomarker of internal dose.

대상 데이터

From June to September 2005, blood plasma was collected for furan analysis from male and female volunteers at Korea University Anam Hospital (Seoul, Korea), according to a protocol approved by the Institute Review Board. The blood samples were obtained from 100 healthy subjects (49 males, 51 females), ages 30 to 70 years. A dietician confirmed that all volunteers consumed a regular Korean diet and did not eat large amounts of furan-containing food items.
Furan (+99%, purity) and d₄-furan (+99%, purity) were purchased from Sigma-Aldrich (St. Louis, MO, USA). High performance liquid chromatography (HPLC) grade of water and methanol (HPLC grade) were supplied by J.

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