Olibanum (Boswellia serrata) has been shown to have anti-inflammatory, anti-arthritic and anticancer effects. This study determined the role of a water extract of olibanum in platelet-derived growth factor (PDGF)-stimulated proliferation and migration of rat aortic smooth muscle cells (RASMCs). PDGF...
Olibanum (Boswellia serrata) has been shown to have anti-inflammatory, anti-arthritic and anticancer effects. This study determined the role of a water extract of olibanum in platelet-derived growth factor (PDGF)-stimulated proliferation and migration of rat aortic smooth muscle cells (RASMCs). PDGF-BB induced the migration and proliferation of RASMCs that were inhibited by olibanum extract in a dose-dependent manner. The PDGF-BB-increased phosphorylation of p38 mitogen-activated protein kinase (MAPK); the heat shock protein (Hsp) 27 was significantly inhibited by the olibanum extract. The effects of PDGF-BB-induced extracellular signal-regulated kinase1/2 was not altered by the olibanum extract. Treatment with olibanum extract inhibited PDGF-BB-stimulated sprout out growth of aortic rings. These results suggest that the water extract of olibanum inhibits PDGF-BB-stimulated migration and proliferation in RASMCs as well as sprout out growth, which may be mediated by the inhibition of the p38 MAPK and Hsp27 pathways.
Olibanum (Boswellia serrata) has been shown to have anti-inflammatory, anti-arthritic and anticancer effects. This study determined the role of a water extract of olibanum in platelet-derived growth factor (PDGF)-stimulated proliferation and migration of rat aortic smooth muscle cells (RASMCs). PDGF-BB induced the migration and proliferation of RASMCs that were inhibited by olibanum extract in a dose-dependent manner. The PDGF-BB-increased phosphorylation of p38 mitogen-activated protein kinase (MAPK); the heat shock protein (Hsp) 27 was significantly inhibited by the olibanum extract. The effects of PDGF-BB-induced extracellular signal-regulated kinase1/2 was not altered by the olibanum extract. Treatment with olibanum extract inhibited PDGF-BB-stimulated sprout out growth of aortic rings. These results suggest that the water extract of olibanum inhibits PDGF-BB-stimulated migration and proliferation in RASMCs as well as sprout out growth, which may be mediated by the inhibition of the p38 MAPK and Hsp27 pathways.
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문제 정의
These findings have suggested that olibanum extract exerts an inhibitory effect on vascular smooth muscle cell behavior. The goal of this study was to investigate the effects of olibanum on RASMC responses with olibanum extract isolated using a water extraction method. The results of this study demon strate, for the first time, that olibanum extract inhibits PDGF-induced migration and proliferation in rat aortic smooth muscle cells.
제안 방법
, 2007); these findings imply that olibanum can exert an inhibitory effect on the MAPK signaling pathway. In this study, we explored the possible role of p38 MAPK and ERK 1/2, and the effects of olibanum on PDGF-induced RASMC migration and proliferation; JNK, an important isoenzyme of MAPK was not included because these two PDGF-in- duced. RASMC responses (proliferation and migration) were not significantly suppressed by JNK inhibition in previous studies (Lee et al.
데이터처리
Data are expressed as means土SEM. The student t test was used to compare the data. Findings with p values < 0.
이론/모형
Cells were treated with or without olibanum extract (1 ~1,000 ㎍/ml) and then stimulated by PDGF-BB (10 ng/ml) for 36 h. Cell proliferation was examined with the BrdU incorporation assay. Proliferation in the quiescent state was considered as 100% (n=10).
성능/효과
The goal of this study was to investigate the effects of olibanum on RASMC responses with olibanum extract isolated using a water extraction method. The results of this study demon strate, for the first time, that olibanum extract inhibits PDGF-induced migration and proliferation in rat aortic smooth muscle cells. These effects were confirmed by the findings of the ex vivo analysis of the formation of sprout윦 from aortic rings.
, 2007). The findings of this study showed that olibanum extract inhibited the PDGF-induced phosphorylation of p38 MAPK in the RASMCs and attenuated RASMC migration induced by PDGF stimulation. Therefore, our data suggest that the suppression of p38 MAPK activity may be responsible for the inhibition of PDGF-increased RASMC migration and proliferation in response to olibanum extract.
In the present study, we found that the PDGF-induced phosphor ylation of Hsp27 was attenuated by treatment with oliba num extract. Therefore, the findings of this study suggest that the olibanum extract-induced inhibition of PDGF- stimulated RASMC migration and proliferation may be as sociated with the p38 MAPK-mediated Hsp27 pathway.
후속연구
These data provide evidence that olibanum exMaet exerts inhibitory effects on PDGF-induced migration and proliferation in RASMCs, and that these inhibitory effects may be induced by the p38 MAPK and/or Hsp27 pathways. The findings of this study, therefore, may provide valuable information for additional research or drug development for the prevention and treatment of vascular diseases such as atherosclerosis. However, further study will be required for elucidating the mecha nism linking olibanum extract and PDGF-stimulated sig nals, in the p38 MAPK and Hsp 27 pathways, associated with RASMC migration and proliferation appears that olibanum extract targets the cell surface because the oliba num extract used in this study was water soluble; this might provide an important clue as to the mechanism in volved in the inhibitory effects of olibanum extract on RASMC migration and proliferation in response to PDGF.
The findings of this study, therefore, may provide valuable information for additional research or drug development for the prevention and treatment of vascular diseases such as atherosclerosis. However, further study will be required for elucidating the mecha nism linking olibanum extract and PDGF-stimulated sig nals, in the p38 MAPK and Hsp 27 pathways, associated with RASMC migration and proliferation appears that olibanum extract targets the cell surface because the oliba num extract used in this study was water soluble; this might provide an important clue as to the mechanism in volved in the inhibitory effects of olibanum extract on RASMC migration and proliferation in response to PDGF. Further studies are needed to clarify the mechanisms.
However, further study will be required for elucidating the mecha nism linking olibanum extract and PDGF-stimulated sig nals, in the p38 MAPK and Hsp 27 pathways, associated with RASMC migration and proliferation appears that olibanum extract targets the cell surface because the oliba num extract used in this study was water soluble; this might provide an important clue as to the mechanism in volved in the inhibitory effects of olibanum extract on RASMC migration and proliferation in response to PDGF. Further studies are needed to clarify the mechanisms.
참고문헌 (31)
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