[논문]Antioxidant Activities of Vaccinium uliginosum L. Extract and Its Active Components

Kim, Young-Hee; Bang, Chae-Young; Won, Eun-Kyung; Kim, Jong-Pyung; Choung, Se-Young

doi:10.1089/jmf.2008.1127

Antioxidant Activities of Vaccinium uliginosum L. Extract and Its Active Components 원문보기

Journal of medicinal food, v.12 no.4, 2009년, pp.885 - 892

Kim, Young-Hee (Department of Hygienic Chemistry and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University) , Bang, Chae-Young (Department of Hygienic Chemistry and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University) , Won, Eun-Kyung (Department of Hygienic Chemistry and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University) , Kim, Jong-Pyung (Korea Research Institute of Bioscience & Biotechnology) , Choung, Se-Young (Department of Hygienic Chemistry and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University)

Abstract ▼ AI-Helper

Vaccinium uliginosum L. (also known as bog bilberry) is a low-growing deciduous shrub classified in the Ericaceae family of plants, which includes numerous Vaccinium berries, blueberries, and cranberries. Berries of the Ericaceae family are known to contain organic acids, vitamins, glycosides, and anthocyanins and have been reported to have antioxidant activity. In order to identify the antioxidative principles of V. uliginosum, we separated water extracts into polyphenol, anthocyanin-rich (pigment), and sugar/acid fractions by using ethyl acetate, acidic methanol (MeOH), and 0.01 N HCl. Antioxidant activities were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide radical, and hydroxyl radical assays. The crude extract and fractions containing polyphenol and pigment exhibited the greatest antioxidant activities with 50% inhibitory concentration ($IC_{50}$) values of $85.8\;{\mu}g/mL$, $33.2\;{\mu}g/mL$, and $16.7\;{\mu}g/mL$, respectively, for the DPPH assay and $48.1\;{\mu}g/mL$, $83.8\;{\mu}g/mL$, and $51.9\;{\mu}g/mL$ for the nonenzymatic superoxide radical assay. The fractions containing polyphenol, pigment, and sugar/acid significantly inhibited xanthine oxidase. To investigate the functional compounds from the active fractions, we purified the polyphenol fraction and separated the compounds by using chromatographic techniques. The crude extract was dissolved in MeOH and further purified by reversed-phase high-performance liquid chromatography (HPLC) using MeOH-water (35:65 vol/vol) (with 0.04% trifluoroacetic acid) to obtain VU-EA-1 (16.6 mg), VU-EA-2 (8.5 mg), VU-EA-3 (19.8 mg), VU-EA-4 (12.8 mg), VU-EA-5 (6.5 mg), and VU-EA-6 (23.5 mg). The MeOH-washed fraction from the HPLC was concentrated and purified by reversed-phase HPLC using MeOH-water (50:50 vol/vol) to give VU-EA-10 (12.4 mg). Antioxidant activity was assessed by DPPH, superoxide radical, and hydroxyl radical assays. The isolated compounds exhibited dose-dependent antioxidant activity with $IC_{50}$ values of $7.6\;{\mu}g/mL$ (VU-EA-10) for the DPPH assay, $67.8\;{\mu}g/mL$ (VU-EA-4) for the nonenzymatic superoxide radical assay, and $3.7\;{\mu}g/mL$ (VU-EA-10) and $7.6\;{\mu}g/mL$ (VU-EA-6) for the enzymatic superoxide radical assay and 30% inhibitory concentration values of $0.58\;{\mu}g/mL$ (VU-EA-1), $0.57\;{\mu}g/mL$ (VU-EA-5), and $0.70\;{\mu}g/mL$ (VU-EA-6) for the hydroxyl radical assay. In conclusion, V. uliginosum had potent antioxidative activity, and flavonoids were isolated as the main active principles.

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Antioxidant Activities of Vaccinium uliginosum L. Extract and Its Active Components 원문보기

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Antioxidant Activities of Vaccinium uliginosum L. Extract and Its Active Components 원문보기

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