Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted wit...
Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted with water (W), ethanol (Eth), water or ethanol after ultra high pressure (WP, EthP), and water or ethanol after an extrusion process (WEx, EthEx). Diphenylpicrylhydrazyl (DPPH) radical scavenging activity and ferric reducing ability showed that all extracts had a significant antioxidant activity. In anti-inflammatory activities, Eth and EthEx significantly inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ independent of cell viability in RAW 264.7 macrophage cells. Inducible NO synthase (iNOS) protein was significantly decreased by EthEx. These findings present that Eth and EthEx extracts of ASvS leaves have anti-inflammatory activities, and EthEx extract suppresses LPS-induced NO through the down-regulation of iNOS and pro-inflammatory cytokines.
Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted with water (W), ethanol (Eth), water or ethanol after ultra high pressure (WP, EthP), and water or ethanol after an extrusion process (WEx, EthEx). Diphenylpicrylhydrazyl (DPPH) radical scavenging activity and ferric reducing ability showed that all extracts had a significant antioxidant activity. In anti-inflammatory activities, Eth and EthEx significantly inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ independent of cell viability in RAW 264.7 macrophage cells. Inducible NO synthase (iNOS) protein was significantly decreased by EthEx. These findings present that Eth and EthEx extracts of ASvS leaves have anti-inflammatory activities, and EthEx extract suppresses LPS-induced NO through the down-regulation of iNOS and pro-inflammatory cytokines.
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제안 방법
Tetrazolium was then concerted in to formazan and absorbance was read at 490 nm using the microplate reader. Each experiment was performed using 8 replicate wells for each group, and was independently carried out in duplicate. Concentration-response curves were plotted and percent inhibition was calculated as: sample absorbance×100/control absorbance.
, Billerica, MA, USA) water was used for the HPLC analysis. HPLC analyses were performed by a Gilson instrument consisting of pump (model 322) and UV detector (model 156) (Gilson Inc., Middleton, WI, USA). Absorbance was detected by Softmax® Pro 5 software connected to a Versamax microplate reader (Molecular Devices, Sunnyvale, CA, USA) Anti-inducible NOS (BD Bioscience, San Diego, CA, USA), anti-COX-2 (Cell Signaling Technology, Beverly, MA, USA) and anti-β-actin (Cell Signaling Technology) primary antibodies were used in Western blotting.
Another 1 kg of leaves was treated with an extrusion process at 155℃ for 2 min (4-5 mm, 35% water contained, 250 rpm). Six extracts were prepared by the mentioned processes and were classified as hot-water extract (W), ethanol extract (Eth), hot-water extract after ultra high pressure work (WP), ethanol extract after ultra high pressure work (EthP), hotwater extract after extrusion processing (WEx), and ethanol extract after extrusion processing (EthEx). All aqueous extracts were dried with a speed vacuum concentrator and used for the following experiments.
대상 데이터
The RAW 264.7 mouse macrophage cells were obtained from the Korea Cell Line Bank (Seoul, Korea), and were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen Gibco®, Carlsbad, CA, USA) without phenol red, and supplemented with 10% fatal bovine serum (FBS, Gibco®) and 1% antibiotics (Gibco®).
데이터처리
All results are represented as mean± standard deviation (SD). Statistical analyses between 2 groups were performed by the independent sample t-test using SPSS 12.0. The data were considered significant at p<0.
이론/모형
The washed cell pellets were re-suspended in PRO-PREP Protein Extraction Solution (iNtRON Biotechnology, Gyeonggi, Korea) and then incubated for 20 min at 4℃. Protein concentration was measured by Bradford method (Bio Rad Laboratories, Melvile, NY, USA). Thirty µg of cellular proteins obtained from the treated and untreated cell extracts were separated on 8% sodium dodecyl sulfate (SDS)-polyacrylamide gels, and then transferred to nitrocellulose membranes.
참고문헌 (50)
Lee SH, Kim BK, Cho SH, Shin KH. Phytochemical constituents from the fruits of Acanthopnax sessiliflorus. Pharm. Res. Bull. 25: 280-284 (2002)
Han SB, Yoon YD, Ahn HJ, Lee HS, Lee CW, Yoon WK, Park SK, Kim HM. Toll-like receptor-mediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. Int. Immunopharmacol. 3: 1301-1312 (2003)
Jung HJ, Park HJ, Kim RG, Shin KM, Ha J, Choi JW, Kim HJ, Lee YS, Lee KT. In vivo anti-inflammatory and antinociceptive effects of liriodendrin isolated from the stem bark of Acanthopanax senticosus. Planta Med. 69: 610-616 (2003)
Jung SM, Schumacher HR, Kim H, Kim M, Lee SH, Pessler F. Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: Elevation of prostaglandin D2 levels. Arthritis Res. Ther. 9: R64 (2007)
Lee S, Son D, Ryu J, Lee YS, Jung SH, Kang J, Lee SY, Kim HS, Shin KH. Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch. Pharm Res. 27: 106-110 (2004)
Xi M, Hai C, Tang H, Chen M, Fang K, Liang X. Antioxidant and antiglycation properties of total saponins extracted from traditional Chinese medicine used to treat diabetes mellitus. Phytother. Res. 22: 228-237 (2008)
Park SH, Lee SG, Kang SK, Chung SH. Acanthopanax senticosus reverses fatty liver disease and hyperglycemia in ob/ob mice. Arch. Pharm. Res. 29: 768-776 (2006)
Apers S, Naessens T, Van Miert S, Pieters L, Vlietinck A. Quality control of roots of Eleutherococcus senticosus by HPLC. Phytochem. Anal. 16: 55-60 (2005)
Kimura Y, Sumiyoshi M. Effects of various Eleutherococcus senticosus cortex on swimming time, natural killer activity, and corticosterone level in forced swimming stressed mice. J. Ethnopharmacol. 95: 447-453 (2004)
Bae EA, Yook OJ, Chang SY, Nohara T, Kim DH. Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Biol. Pharm. Bull. 24: 582-585 (2001)
Jung HJ, Nam JH, Choi JW, Lee KT, Park HJ, Antiinflammatory effects of chiisanoside and chiisanogenin obtained from the leaves of Acanthopanax chiisanensis in the carrageenan- and Freund's complete adjuvant-induced rats. J. Ethnopharmacol. 97: 359-367 (2005)
Won JH, Park SY, Nam SG, Park HJ, Choi JW, Lee KT. Inhibition of lipopolysaccharide-induced expression of inducible nitric oxide and cyclooxygenase-2 by chiisanoside via suppression of nuclear factor-κb activation in RAW 264.7 macrophage cells. Biol. Pharm. Bull. 28: 1919-1924 (2005)
Yi JM, Kim MS, Seo SE, Lee KN, Yook CS, Kim HM. Acanthopanax senticosus root inhibit mast cell-dependent anaphylaxis. Clin. Chim. Acta 312: 163-168 (2001)
Ha ES, Hwang SH, Shin KS, Yu KW, Lee KH, Choi JS, Park WM, Yoon TJ. Anti-metastatic activity of Acanthopanax senticosus extract and its possible immunological mechanism of action. J. Ethnopharmacol. 93: 247-253 (2004)
Lin QY, Jin LJ, Ma YS, Shi M, Xu YP. Acanthopanax senticosus inhibits nitric oxide production in murine macrophages in vitro and in vivo. Phytother. Res. 21: 879-883 (2007)
Lin QY, Jin LJ, Cao ZH, Lu YN, Xue HY, Xu YP. Acanthopanax senticosus suppresses reactive oxygen species production by mouse peritoneal macrophages in vitro and in vivo. Phytother. Res. 22: 740- 745 (2008)
Lin QY, Jin LJ, Cao ZH, Xu YP. Inhibition of inducible nitric oxide synthase by Acanthopanax senticosus extract in RAW 264.7 macrophages. J. Ethnopharmacol. 118: 231-236 (2008)
Lim SY, Leem JY, Lee CS, Jang YJ, Park JW, Yoon S. Antioxidant and cell proliferation effects of Acanthopanax senticosus extract in human osteoblast-like MG-63 cell line. Korean J. Food Sci. Technol. 39: 694-700 (2007)
Tohda C, Ichimura M, Bai Y, Tanaka K, Zhu S, Komatsu K. Inhibitory effects of Eleutherococcus senticosus extracts on amyloid β (25-35)-induced neuritic atrophy and synaptic loss. J. Pharmacol. Sci. 107: 329-339 (2008)
Lee SH, Son DW, Ryu JY, Lee YS, Jung SH, Kang JG, Lee SY, Ki HS, Shin KH. Anti-oxidant activities of Acanthopanax senticosus stems and their lignan components. Arch. Pharm. Res. 27: 106-110 (2004)
Kim IH, Kim SH, Kwon JH. Optimizing the hot-water extraction conditions for Acanthopanacis cortex using response surface methodology. J. Korean Soc. Food Sci. Nutr. 37: 512-520 (2008)
Lim S, Leem JY, Lee C, Jang Y, Park J, Yoon S. Antioxidant and cell proliferation effects of Acanthopanax senticosus extract in human osteoblast-like MG-63 cell line. J. Korean Soc. Food Sci. Nutr. 39: 694-700 (2007)
Masuda T, Yonemori S, Oyama Y, Takeda T, Tanaka T, Andoh T, Sinohara A, Nakata M. Evaluation of the antioxidant activity of environmental plants: Activity of the leaf extracts from seashore plants. J. Agr. Food Chem. 47: 1749-1754 (1999)
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of 'antioxidant power': The FRAP assay. Anal. Biochem. 139: 70-76 (1996)
Tute'yan AV, Klebanoc GI, Il'ina SE, Lyubitskii OB. Comparative study of antioxidant properties of immunoregulatory peptides. B. Exp. Biol. Med+. 136: 155-158 (2003)
Chen TS, Liou SY, Chang YL. Antioxidant evaluation of three adaptogen extracts. Am. J. Chinese Med. 36: 1209-1217 (2008)
Lee S, Shin DS, Oh KB, Shin KH. Antibacterial compounds from the leaves of Acanthopanax senticosus. Arch. Pharm. Res. 26: 106- 110 (2003)
Li Q, Jia Y, Xu L, Wang X, Shen Z, Liu Y, Bi K. Simultaneous determination of protocatechiuc acid, syringin, chlorogenic acid, caffeic acid, liriodendrin, and isofraxidin in Acanthopanax senticosus Harms by HPLC-DAD. Biol. Pharm. Bull. 29: 532-534 (2006)
Zhang X, Morrison DC. Lipopolysaccharide-induced selective priming effects on tumor necrosis factor a and nitric oxide production in mouse peritoneal macrophages. J. Exp. Med. 177: 511-516 (1993)
Kim MS, Kim SO, Lim WK, Lee JM, Hwang CY, Cho KH, Kim HM. Novel effects on On-Chung-Eum, the traditional plant medicine, on cytokine production in human mononuclear cells from Beh $\c{c}$ et's. Immunopharm. Immunot. 25: 65-72 (2003)
Morgan MM, Clayton CC, Heinricher MM. Dissociation of hyperalgesia from fever following intracerebroventricular administration of interleukin-1beta in the rat. Brain Res. 1022: 96- 100 (2004)
Lee YJ, Chun HY, Kwak HK, Yoon S. The effects of A. senticosus supplementation on serum lipid profiles, biomarkers of oxidative stress, and lymphocyte DNA damage in postmenopausal women. Biochem. Bioph. Res. Co. 375: 44-48 (2008)
Won JH, Park SY, Nam SG, Park HJ, Choi JW, Lee KT. Biologically active triterpenoid saponins from Acanthopanax senticosus. J. Nat. Prod. 69: 1577-1581 (2006)
Jin JL, Lee S, Lee YY, Kim JM, Heo JE, Yun-Choi HS. Platelet anti-aggregating triterpenoids from the leaves of Acanthopanax senticosus and the fruits of A. sessiliflorus. Planta Med. 70: 564-566 (2004)
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