프로폴리스를 기능성 식품 재료로 활용하기 위한 기초자료로 지역에 따른 일반성분, 총 페놀성 화합물 함량 및 항산화 활성을 조사하였다. 일반성분 중 조지방이 가장 많이 함유되어 있었고, 조섬유가 가장 적게 함유되어 있었다. 총 페놀성 화합물은 거창산 에탄올과 물 추출물, 제주산 에탄올과 물 추출물에서 각각 184.17, 316.19, 204.33 및 47.83 mg/g으로 다른 추출물에 비해 거창산 물 추출물에서 총 페놀성 화합물이 가장 높았다. 프로폴리스 추출물을 이용하여 라디칼 소거활성, 환원력 및 FTC 방법을 이용하여 여러 가지 항산화 활성을 측정한 결과 모든 추출물에서 농도의존적인 경향을 보였으며, 거창산 물 추출물에서 가장 높은 항산화 활성을 보였다. 따라서 거창산 프로폴리스 물 추출물은 높은 항산화 활성을 바탕으로 기능성식품 소재로 활용할 가능성이 매우 높을 것으로 판단된다.
To obtain basic information on the potential use of propolis as a raw material in functional food, proximate composition, total phenolics content and antioxidant activities of different propolis extracts in Korea were investigated. Propolis had the highest level of crude fat and the lowest level of crude fiber. The total phenolics content of ethanol and water extract of propolis from Geochang (GEE and GWE), ethanol and water extract of propolis from Jeju (JEE and JWE) were 184.17, 316.19, 204.33 and 47.83 mg gallic acid equivalent/g, respectively. GWE contained relatively higher levels of total phenolics than the other extracts. The antioxidant potential of the extracts was assessed by different in vitro assays such as DPPH, ABTS, reducing power, ferric reducing/antioxidant power (FRAP) and peroxidation inhibiting activities through linoleic acid emulsion system. DPPH and ABTS radical scavenging activities of all the extracts were dose dependent. The GWE exhibited the best performance in reducing power, FRAP, and lipid peroxidation using ferric thiocyanate (FTC) assay. These results demonstrated that GWE has excellent antioxidant activities and thus it has great potential as a raw material for functional food.
Rubio OC, Cuella AC, Rojas N, Castro HV, Rastrelli L,
Aquino R. 1999. A polyisoprenylated benzophenone from Cuban propolis. J Nat Prod 62: 1013-1015.
Marcucci MC. 1995. Propolis: chemical composition, biological properties and therapeutic activity. Apodologie 26:83-99.
Bonvehi JS, Coll FV, Jorda RE. 1994. The composition, active components bacteriostatic activity of propolis in dietetics.
J Am Oil Chem Soc 71: 529-532.
Greenaway W, May J, Scaysbrook T, Whatley FR. 1990.
Identification by gas chromatography-mass spectrometry
of 150 compounds in propolis. Z Naturforsch 46c: 111-121.
Kalogeropoulos N, Konteles SJ, Troullidou E, Mourtzinos
I, Karathanos VT. 2009. Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chem 116: 452-461.
Banskota AH, Tezuka Y, Adnyana IK, Midorikawa K,
Matsushige K, Message D, Huertas AAG, Kadota S. 2000.
Cytotoxic, hepatoprotective and free radical scavenging effects
of propolis from Brazil, Peru, the Netherlands and
China. J Ethnolpharmacol 72: 239-246.
Dobrowolski JW, Vohora SB, Sharma K, Shah SA, Naqvi
SAH, Dandiya PC. 1991. Antibacterial, antifungal, antiamoebic,
antiinflammatory and antipyretic studies on propolis
bee products. J Ethnopharmacol 35: 77-82.
Burdoc GA. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food Chem Toxicol 36: 347-363.
Gekker G, Hu S, Spivak M, Lokensgard JR, Peterson PK. 2005. Anti-HIV-1 activity of propolis in $CD4^+$ lymphocyte and microglial cell cultures. J Ethnophamacol 102: 158-163.
Lee HJ, Bae YI, Jeong CH, Shim KH. 2005. Biological activities of various solvent extracts from propolis. J Korean Soc Food Sci Nutr 34: 1-7.
Choi YM, Noh DO, Cho SY, Suh HJ, Kim KM, Kim JM.
2006. Antioxidant and antimicrobial activities of propolis
from several regions of Korea. LWT-Food Sci Technol 39:
Jang IW, Park JS, Kwon HC, Jung MY, Choi DS. 2009. Antimutagenic and antibacterial activities of Korean and American propolis. Korean J Food Sci Technol 41: 694-699.
Kumazawa S, Hamasaka T, Nakayama T. 2004. Antioxidant activity of propolis of various geographic origins. Food Chem 84: 329-339.
AOAC. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, DC, USA.
Kim DO, Jeong SW, Lee CY. 2003. Antioxidant capacity
of phenolic phytochemical from various cultivars of plums.
Blois MA. 1958. Antioxidant determination by the use of a stable free radical. Nature 181: 1199-1200.
Pellegrini N, Re R, Yang M, Rice-Evans CA. 1999. Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activities applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Methods Enzymol 299: 379-389.
Oyaizu M. 1986. Studies on products of browning reaction: antioxidant activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44: 307-315.
Liu L, Sun Y, Laura T, Liang X, Ye H, Zeng X. 2009. Determination of polyphenolic content and antioxidant activity of kudingcha made from Ilex kudingcha C.J. Tseng. Food Chem 112: 35-41.
Lee JY, Hwang WI, Lim ST. 2004. Antioxidant and anticancer activities of organic extracts from Platycodon grandiflorum A. De Candolle roots. J Ethnopharmacol 93: 409-415.
Kim JT, Kim CJ, Cho YJ, Choi AJ, Shin WS. 2002. Characteristics of propolis extracts from ethanol extraction.Korean J Food Sci Technol 34: 941-946.
Boo HO, Lee HH, Lee JW, Hwang SJ, Park SU. 2009. Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean J Medicinal Crop Sci 17: 15-20.
Jeong CH, Bae YI, Lee HJ, Shim KH. 2003. Chemical components
of propolis and its ethanolic extracts. J Korean Soc Food Sci Nutr 32: 501-505.
Ahn MR, Kumazawa S, Usui Y, Kakamura J, Matsuka M, Zhu F, Nakayama T. 2007. Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem 101: 1383-1392.
Moreira L, Dias LG, Pereira JA, Estevinho L. 2008. Antioxidant
properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem Toxicol 46: 3482-3485.
Mohammadzadeh S, Sharriatpanahi M, Hamedi M, Amanzadeh
Y, Ebrahimi SES, Ostad SN. 2007. Antioxidant power
of Iranian propolis extract. Food Chem 103: 729-733.
Yoshino M, Murakami K. 1998. Interaction of iron with polyphenolic compounds, application to antioxidant characterization. Anal Biochem 257: 40-44.
이 논문을 인용한 문헌 (2)
Yang, Ya-Ru ; Park, Yang-Kyun 2011. "Comparison of Antioxidant Activities of Black Onion Extracts" 한국식품저장유통학회지 = Korean journal of food preservation, 18(6): 954~960
Kim, Sang-A ; Chung, Hyun-Jung 2013. "Antimicrobial Effects of Propolis against Oral Microorganisms" 한국식품과학회지 = Korean journal of food science and technology, 45(3): 370~375