[논문]병원내에서 분리된 메티실린내성 황색포도상구균에 대한 무화과잎 추출물의 항미생물효과

이영수; 차정단

병원내에서 분리된 메티실린내성 황색포도상구균에 대한 무화과잎 추출물의 항미생물효과
Synergistic Antibacterial Activity of Fig (Ficus carica) Leaves Extract Against Clinical Isolates of Methicillin-resistant Staphylococcus aureus 원문보기

한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, v.38 no.4, 2010년, pp.405 - 413

초록
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무화과는 뽕나무 과에 속하며 세계에서 가장 오래된 과일 중 하나이고, 국내에서는 주로 소화장애, 염증성궤양 그리고 가려움증 등에 사용되어 왔다. 본 연구에서는 무화과 잎을 메탄올로 추출한 후 임상진료실에서 분리된 메티실린 내성 황색포도상구균(MRSA)에 대한 항균활성을 보았다. 그 결과 분리된 MRSA 1-20에서 최소억제농도 (MIC)는 2.5 mg/mL에서 20 mg/mL 농도로 측정되었으며, 최소살균농도(MBC)는 5 mg/mL에서 20 mg/mL 농도로 관찰되었다. Oxacillin이나 ampicillin과 병용투여 시 4-8배 이상의 MIC/MBC농도가 감소되었으며, FICI 값도 0.0375-0.5이하로 병용효과를 나타내었다. 더불어 메탄올추출물 단독 사용시 보다 항생제와 병용투여 시 빠른 사멸효과를 보였다. 결론적으로 무화과 잎이 메티실린 내성 황색포도상구균에 항균활성을 나타냄을 확인하였으며, 메티실린 내성 황색포도상구균 감염에 천연 치료제로서의 가능성을 확인하였다.

Abstract ▼ AI-Helper

Fig (Ficus carica L.) belongs to the mulberry tree (Moraceae) which is one of the oldest fruits in the world. It has been used as a digestion promoter and a cure for ulcerative inflammation and eruption in Korea. The present study investigated the antimicrobial activity of methanol (MeOH) extract of fig leaves against methicillin-resistant Staphylococcus aureus (MRSA) isolated in clinic. The MeOH extract (MICs, 2.5 to 20 mg/mL; MBCs, 5 to 20 mg/mL) was demonstrated as antibacterial activity in isolates MRSA 1-20. The administration of the MeOH extract in combination with oxacillin or ampicillin induced a reduction of ${\geq}$4-8-fold in all tested bacteria, which was considered to be synergistic based on a FICI of ${\leq}$0.375-0.5. Furthermore, time-kill study was found that a combination of MeOH extract with oxacillin or ampicillin produced a more rapid decrease in the concentration of bacteria CFU/mL than MeOH extract alone. The results suggest that fig leaves could be employed as a natural antibacterial agent in MRSA infection care products.

주제어

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제안 방법

0×10⁷ CFU/mL were exposed to the MBC of the MeOH extract alone, or with ampicillin or oxacillin. All experiments were repeated several times and colony counts were conducted in triplicate, after which the means were determined.
The bactericidal activities of the drugs evaluated in this study were also evaluated using time-kill curves constructed using the isolated and reference strains. Tubes containing the MeOH extract and bacteria were incubated at 37°C, and viable counts were conducted at 0, 0.
Tubes containing the MeOH extract and bacteria were incubated at 37°C, and viable counts were conducted at 0, 0.5, 1, 2, 3, 4, 5, 6, 12, and 24 h after the addition of the antimicrobial agents by plating aliquots of the samples on agar and subsequent incubation for 24 hr at 37°C.

대상 데이터

Bong-Seop Kil, College of Natural Science, Wonkwang University. The voucher specimens (DJ-05-F1) were deposited at the Herbarium of the College of Natural Science, Wonkwang University. The dried and powered leaves (1.

성능/효과

The minimum inhibitory concentration (MIC) was determined as the lowest concentration of test samples that resulted in a complete inhibition of visible growth in the broth. Following 10% CO₂ incubation of MIC plates, the minimum bactericidal concentration (MBC) was determined on the basis of the lowest concentration of the MeOH extract that kill 99.9% of the test bacteria by plating out onto each appropriate agar plate.
Phenolic and flavonoid compounds constitute an important class of phytochemicals which possess diverse biological activities like astringent, antioxidant, anticancer, anti-inflammation, and antibacterial activity, etc [5, 8, 20, 21, 23]. The results of the antibacterial activity showed that the MeOH extract of fig leaves exhibited inhibitory activities against isolates MRSA and reference strains (MIC, 2.5 to 20 mg/mL; MBC, 5 to 20 mg/mL). The synergistic effect of MeOH extract with oxacillin or ampicillin was shown as reduced ≥4-8-fold in most of tested MRSA, producing a synergistic effect as defined by FICI ≤ 0.

참고문헌 (25)

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