다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기
Journal of plant biotechnology = 식물생명공학회지, v.37 no.2, 2010년, pp.115 - 124
조용구 (충북대학교 식물자원학과) , 우희종 (국립농업과학원) , 윤웅한 (국립농업과학원) , 김홍식 (충북대학교 식물자원학과) , 우선희 (충북대학교 식물자원학과)
AI-Helper
As the completion of genome sequencing, large collection of expression data and the great efforts in annotating plant genomes, the next challenge is to systematically assign functions to all predicted genes in the genome. Functional genome analysis of plants has entered the high-throughput stage. Th...
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
| 핵심어 | 질문 | 논문에서 추출한 답변 |
|---|---|---|
| 벼의 특징? | 벼는 세계인구의 반 이상이 주식으로 이용하고 있는 가장 중요한 식량작물의 하나로서 n=12의 염색체를 갖는 이배체 단자엽식물이며 그 게놈의 크기가 430 Mb(Arumuganathan and Earle 1991)로서 비교적 작아 분자유전연구의 모델식물로 이용되고 있다. 특히 벼는 다른 곡류 작물들과 유전자의 높은 상동성을 나타내며 염색체 상의 유전자 배열이 유사하여 유전자 연구에 매우 좋은 작물로서 이용되고 있다. | |
| Post Genome 시대의 연구방향은 유전자 기능분석 연구에 집중되는 이유? | 21세기 초에 선진국을 중심으로 초파리(Drosophila melanogaster; Science 287, 2000), 애기장대(Arabidopsis thaliana; Nature 408, 2000), 인체(Homo sapiens; Nature 409, 2001), 벼(Oryza sativa; Nature 436, 2005), 콩(Glycine max; Nature 463, 2010) 등 유전체 전 염기서열 해독 프로젝트가 완료되었으며 이후 이들 동식물의 유전자 기능분석 연구가 활발히 수행되고 있다. 최근 들어 모델식물로 벼와 애기장대를 이용한 구조 유전체 및 비교 유전체의 비약적인 연구 성과에도 불구하고, 게놈상의 유전자들 중 약 60% 이상이 아직 기능이 밝혀지지 않은 상태로 놓여 있다. 따라서 Post Genome 시대의 연구방향은 유전자 기능분석 연구에 집중되고 있는 실정이다. | |
| 유전자 연구에 매우 좋은 작물로 벼가 이용되는 이유? | 벼는 세계인구의 반 이상이 주식으로 이용하고 있는 가장 중요한 식량작물의 하나로서 n=12의 염색체를 갖는 이배체 단자엽식물이며 그 게놈의 크기가 430 Mb(Arumuganathan and Earle 1991)로서 비교적 작아 분자유전연구의 모델식물로 이용되고 있다. 특히 벼는 다른 곡류 작물들과 유전자의 높은 상동성을 나타내며 염색체 상의 유전자 배열이 유사하여 유전자 연구에 매우 좋은 작물로서 이용되고 있다. |
Adams MD, Celniker SE, Holt RA, et al. (2000) The genome sequence of Drosophila melanogaster. Science 287:2185-2195
Adham AR, Zolman BK, Millius A, Bartel B (2005) Mutations in Arabidopsis acyl-CoA oxidase genes reveal distinct and overlapping roles in beta-oxidation. Plant J 41:859-874
Azpiroz-Leehan R, Feldmann KA (1997) T-DNA insertion mutagenesis in Arabidopsis: going back and forth. Trends Genet 13:152-156
Arumuganathan K and Earle ED (1991) Estimation of nuclear DNA content of plants by flow cytometry. Plant Mol Bio Rep 9:229-233
Baulcombe DC (1996) RNA as a target and an initiator of post-transcriptional gene silencing in transgenic plants. Plant Mol Biol. 32:79-82
Bechtold N, Ellis J, Pelletier G (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. C R Acad Sci Ser III 316:1194？1199
Brummell DA, Balint-Kurti P, Harpster MH, et al. (2003) Inverted repeat of a heterologous 3'-untranslated region for highefficiency, high-throughput gene silencing. Plant J. 33:798-800
Chin HG, Choe MS, Lee SH, Park SH, Koo JC, Kim NY, et al. (1999) Molecular analysis of rice plants harboring an Ac/Ds transposable element-mediated gene trapping system. Plant J 19:615-623
Degenkolbe T, Do PT, Zuther E, Repsilber D, Walther D, Hincha DK, Kohl KI (2009) Expression profiling of rice cultivars differing in their tolerance to long-term drought stress. Plant Mol Biol. 69:133-153
Fields, S (2001) Proteomics. Proteomics in genomeland. Science 291:1221-1224
Frary A, Nesbitt TC, Grandillo S, Knaap E, Cong B, Liu J, Meller J, Elber R, Alpert KB, Tanksley SD (2000) Fw2.2: a quantitative trait locus key to the evolution of tomato fruit size. Science 289:85-88
Furutani I, Sukegawa S, Kyozuka J (2006) Genome-wide analysis of spatial and temporal gene expression in rice panicle development. Plant J. 46:503-511
Gilliland LU, McKinney EC, Asmussen MA, Meagher RB (1998) Detection of deleterious genotypes in multigenerational studies. I. Disruptions in individual Arabidopsis actin genes. Genetics 149:717-725
Hirochika H (2001) Contribution of the Tos17 retrotransposon to rice functional genomics. Curr. Opin. Plant Biol. 4:118-122
Hirsch RE, Lewis BD, Spalding EP, Sussman MR (1998) A role for AKT1 potassium channel in plant nutrition. Science 280:918-921
Ichikawa T, Nakazawa M, Kawashima M, Iizumi H, Kuroda H, et al. (2006) The FOX hunting system: an alternative gainoffunction gene hunting technique. Plant J. 48:974-985
International Human Genome Sequencing Consortium (2001) Initial sequencing and analysis of the human genome. Nature 409:860-921
International Rice Genome Sequencing Project (2005) The mapbased sequence of the rice genome. Nature 436:793-800
Jain M, Khurana JP (2009) Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice. FEBS J. 276:3148-3162
Jander G, Baerson SR, Hudak JA, et al. (2003) Ethyl methaesulfonate saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance. Plant Physiol. 131:139-146
Jeon JS, Lee s, Jung KH, Jun SH, Jeong DH, Lee J, Kim C, Jang S, et al. (2000) T-DNA insertional mutagenesis for functional genomics in rice. Plant J 22:561-570
Jiang SY and Ramachandran S (2010) Natural and artificial mutants as valuable resources for functional genomics and molecular breeding. Int J Biol Sci 6:228-251
Jorgensen RA, Que Q, Stam M (1999) Do unintended antisense transcripts contribute to sense co-suppression in plants? Trends Genet. 15:11-12
Jung KH, An G, Ronald PC (2008) Towards a better bowl of rice: assigning function to tens of thousands of rice genes. Nat Rev Genet 9:91-101
Kagawa T, Sakai T, Suetsugu N, Oikawa K, Ishiguro S, Kato T, Tabata S, Okada K, Wada M (2001) Arabidopsis NPL1: a phototropin homolog controlling the chloroplast high-light avoidance response. Science 291:2138-2141
Kempin SA, Liljegren SJ, Block LM, Rounsley SD, Lam E, Yanofsky MF (1997) Inactivation of the Arabidopsis AGL5 MADS-box gene by homologous recombination. Nature 389:802-803
Kikuchi S, Satoh K, Nagata T, Kawagashira N, et al. (2003) Collection, Mapping, and Annotation of Over 28,000 cDNA Clones from Japonica Rice, Science 301:376-379
Krieg DR (1963) Ethyl methanesulfonate-induced reversion of bacteriophage T4rII mutants. Genetics 48:561-580
Kusaba M (2004) RNA interference in crop plants. Curr Opin Biotechnol. 15:139-143
Lee GS, Park SH, Yun DW, Ahn BO, Kim CG, Han CD, Yi GW, Park DS, Eu MY, Yoon WH (2010) Current status of Ac/Ds mediated Gene Tagging Systems for Study of Rice Functional Genomics in Korea. Journal of Plant Biotechnology 3(2):125-132
Lee TH, Kim YK, Pham TT, Song SI, Kim JK, et al. (2009) RiceArrayNet: a database for correlating gene expression from transcriptome profiling, and its application to the analysis of coexpressed genes in rice. Plant Physiol. 151:16-33
Leonhardt N, Kwak JM, Robert N, Waner D, Leonhardt G, Schroeder JI (2004) Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant. Plant Cell 16:596-615
Li X, Cordero I, Caplan J, Molhoj M, Reiter WD (2004) Molecular analysis of 10 coding regions from Arabidopsis that are homologous to the MUR3 xyloglucan galactosyltransferase. Plant Physiol 134:940-950
Martienssen RA (1998) Functional genomics: probing plant gene function and expression with transposons. Proc Natl Acad Sci USA 95:2021-2026
Martin GB, Brommonschenkel SH, Chunwongse J, Frary A, Ganal MW, Spivey R, Wu T, Earle ED, Tanksley SD (1993) Map-based cloning of a protein kinase gene conferring disease resistance in tomato. Science 262:1432-1436
McCallum CM, Comai L, Greene EA, et al. (2000) Targeting induced local lessions in genomes (TILLING) for plant functional genomics. Plant Physiol. 123:439-442
McKinney EC, Ali N, Traut A, Feldmann KA, Belostotsky DA, McDowell JM, Meagher RB (1995) Sequence-based identification of T-DNA insertion mutations in Arabidopsis: actin mutants act2-1 and act4-1. Plant J 8:613-622
Miyao A, Tanaka K, Murata K, Sawaki H, Takeda S, Abe K, Shinozuka Y, Onosato K, Hirochika H (2003) Target site specificity of the Tos17 retrotransposon shows a preference for insertion within genes and against insertion in retrotransposon rich regions of the genome. Plant Cell 15:1771-1780
Nakamura H, Makoto H, Kou A, Akio M, Naoko T, Mariko K, et al. (2007) A genomewide gain-of-function analysis of rice genes using the FOXhunting system. Plant Mol. Biol. 65:357-371
Park DS, Park SK, Han AI, Wang HJ, Jun NS, Manigbas NL, et al. (2009) Genetic variation through Dissociation(Ds) insertional mutagenesis system for rice in Korea : progress and current ststus. Molecular Breeding 24:1-15
Rensink WA, Buell CR. (2005) Microarray expression profiling resources for plant genomics. Trends Plant Sci. 10:603-609
Rice Annotation Project (2007) Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana. Genome Res 17:175-183
Satoh K, Doi K, Nagata T, Kishimoto N, Suzuki K, Otomo Y, et al. (2007) Gene organization in rice revealed by full-length cDNA mapping and gene expression analysis through microarray. PLoS One. 2:e1235
Salmeron JM, Oldroyd GE, Rommens CM, Scofield SR, Kim SR, Lavelle DT, Dahlbeck D, Staskawicz BJ (1996) Tomato Prf is a member of leucine-rich repeat class of plant disease resistance genes and lies embedded within the Pto kinase gene cluster. Cell 86:123-133
Schmutz J, Cannon SB, Schlueter J, Ma J, Mitros T, Nelson W, et. al. (2010) Genome sequence of the palaeopolyploid soybean. Nature 463:178-183
Schreiber SL (2003) The small-molecule approach to biology: chemical genetics and diversity-oriented organic synthesis make possible the systematic exploration of biology. Chem Eng News 81:51-61
Seki M, Masakazu S, Tetsuya S, Kenji A, Kei I, Junko I, et al. (2004) RIKEN arabidopsis fulllenth (RAFL) cDNA and its applications for expression profiling under abiotic stress conditions. J. Experimental Botany 55:213-223
Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot. 58:221-227
Somerville C and Dangl J (2000) Plant Biology in 2010. Science 290:2077-2078
Song WY, Wang GL, Chen LL, Kim HS, Pi LY, et al. (1995) A receptor kinase-like protein encoded by the rice disease resistance gene Xa21. Science 270:1804-1806
Swarbreck D, Wilks C, Lamesch P, Berardini TZ, et al. (2008) The Arabidopsis Information Resource (TAIR): gene structure and function annotation. Nucleic Acids Res. 36(Database issue):D1009-14
Sweeney MT, Thomson MJ, Pfeil BE, McCouch SR (2006) Caught red-handed: Rc encodes a basic helix-loop-helix protein conditioning red pericarp in rice. Plant Cell 18:283-294
Sweeney MT, Thomson MJ, Cho YG, Park YJ, Wiliamson SH, et al. (2007) Golbal dissemination of a single mutation conferring white pericarp in rice. PLoS Genetics 3(8):e133.doi:10.1371
The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796-815
Till BJ, Cooper J, Tai TH, et al. (2007) Discovery of chemically induced mutations in rice by TILLING. BMC Plant Biol. 7:19
Triques K, Sturbois B, Gallais S, et al. (2007) Characterization of Arabidopsis thaliana mismatch specific endonucleases: application to mutation discovery by TILLING in pea. Plant J. 51:1116-1125
Triques K, Piednoir E, Dalmais M, et al. (2008) Mutation detection using ENDO1: application to disease diagnostics in humans and TILLING and Eco-TILLING in plants. BMC Mol Biol. 9:42-51
Vitha S, Froehlich JE, Koksharova O, Pyke KA, van Erp H, Osteryoung KW (2003) ARC6 is a J-domain plastid division protein and an evolutionary descendant of the cyanobacterial cell division protein Ftn2. Plant Cell 15:1918-1933
Wesley SV, Helliwell CA, Smith NA, et al. (2001) Construct design for efficient, effective and high-throughput gene silencing in plants. Plant J. 27:581-590
Wilkins MR, Sanchez JC, Gooley AA, Appel RD, Humphery-Smith I, Hochstrasser DF, Williams KL (1996) Progress with proteome projects: why all proteins expressed by a genome should be identified and how to do it. Biotechnol Genet Eng Rev 13:19-50
Wu JL, Wu C, Lei C, et al. (2005) Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol. 59:85-97
Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T, Yamamoto K, Umehara Y, Nagamura Y, Sasaki T (2000) Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell 12: 2473-2484
Zheng Z, Mosher SL, Fan B, Klessig DF and Chen Z (2007) Functional analysis of Arabidopsis WRKY25 transcription factor in plant defense against Pseudomonas syringae. BMC Plant Biology 7:1-13
Zolman BK, Martinez N, Millius A, Adham AR, Bartel B (2008) Identification and characterization of Arabidopsis indole-3-butyric acid response mutants defective in novel peroxisomal enzymes. Genetics 180:237-251
*원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다.
출판사/학술단체 등이 한시적으로 특별한 프로모션 또는 일정기간 경과 후 접근을 허용하여, 출판사/학술단체 등의 사이트에서 이용 가능한 논문
Copyright KISTI. All Rights Reserved.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.