[논문]Screening of Antiviral Medicinal Plants against Avian Influenza Virus H1N1 for Food Safety

Lee, Jang-Hyun; Van, Nguyen Dinh; Ma, Jin-Yeul; Kim, Young-Bong; Kim, Soo-Ki; Paik, Hyun-Dong

doi:10.5851/kosfa.2010.30.2.345

Screening of Antiviral Medicinal Plants against Avian Influenza Virus H1N1 for Food Safety 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.30 no.2, 2010년, pp.345 - 350

Lee, Jang-Hyun (Division of Animal Life Science and Bio) , Van, Nguyen Dinh (Division of Animal Life Science and Bio) , Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institute of Oriental Medicine) , Kim, Young-Bong (Division of Animal Life Science and Bio) , Kim, Soo-Ki (Division of Animal Life Science and Bio) , Paik, Hyun-Dong (Division of Animal Life Science and Bio)

Abstract ▼ AI-Helper

Various extracts from 30 medicinal plants were evaluated for their antiviral activity against influenza virus A/Puerto Rico/8/34 (H1N1) and cytotoxicity in MDCK cell culture. The plant material (30 g) was extracted with methanol (300 mL) at room temperature for 24 h, after which the methanolic extracts were filtered, evaporated, and subsequently lyophilized. Evaluation of the potential antiviral activity was conducted by a viral replication inhibition test. Among these medicinal plants, Tussilago farfara, Brassica juncea, Prunus armeniaca, Astragalus membranaceus, Patrinia villosa, and Citrus unshiu showed marked antiviral activity against influenza virus A/H1N1 at concentrations ranging from 0.15625 mg/mL to 1.25 mg/mL, 0.3125 mg/mL to 10 mg/mL, 5 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, 0.625 mg/mL to 10 mg/mL, and 0.3125 mg/mL to 5 mg/mL, respectively. The extracts of Tussilago farfara showed cytotoxicity at concentrations greater than 2.5 mg/mL, whereas the other five main extracts showed no cytotoxicity at concentrations of 10 mg/mL. Taken together, the present results indicated that methanolic extracts of the six main plants might be useful for the treatment of influenza virus H1N1.

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문제 정의

1. Procedure to prepare extracts from medicinal plants in this study.
The aim of this study was to determine the antiviral activity of various medicinal plants against influenza A virus subtype H1N1.

제안 방법

The cell sheet was incubated with virus growth media containing serially diluted extract at 37°C for 48 h until viral CPE was visible. At each sheet, controls infected with 100 TCID₅₀ of virus and mock controls uninfected and untreated were performed in all experiments.

이론/모형

The virus titer was estimated by cytopathic effect (CPE) of cells induced by viral infection and expressed as 50% tissue culture infectious doses (TCID₅₀). TCID₅₀ calculation was described by Reed and Muench method (Del Barrio and Parra, 2000).
Virus titration was performed by the end-point dilution method, using a 96-well microtitre plate with 6 wells per dilution and using 10-fold dilution method. The virus titer was estimated by cytopathic effect (CPE) of cells induced by viral infection and expressed as 50% tissue culture infectious doses (TCID₅₀).

성능/효과

Different parts of 30 medicinal plants were tested for their antiviral activity and cytotoxicity. Of the extracts tested in this study, 6 major plants (Fig. 2), Tussilago farfara, Brassica juncea, Prunus armeniaca, Astragalus membranaceus, Patrinia villosa, and Citrus unshiu, were found to have antiviral activity against influenza A/Puerto Rico/8/34 (H1N1) at a non-toxic concentration to the MDCK cell lines. The results of 6 major plant extracts tested against viruses are shown in Table 2.

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