선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- In this study, we cloned the full-length G푼 cDNA from the kidney of black porgy and investigated the expression pattern of GR mRNA in various tissues (gills, kidney, and intestine) after transfer to a hypoosmotic environment as well as an environment with a high water temperature. In addition, we examined the extent of stress due to changes in osmotic and temperature conditions by analyzing plasma parameters.
- The primers for QPCR were designed based on known sequences of black porgy GR: GR forward primer (5'・CGT TGA TGC AGC CAG GAC AGG-37), GR reverse primer (5Z-GGA ACT GCT GCT GAA CCC TCT G・3‘), p-actin forward primer (5Z-TCG AGC ACG GTA TTG TGA CC-3Z), and P-actin reverse primer (5Z-ACG GAA CCT CTC ATT GCC GA-3'). PCR amplification was performed using an iCycler iQ Multicolor Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and iQ™ SYBR Green Supermix (Bio-Rad) according to the manufacturer's instructions. The QPCR conditions were 1 cycle of denaturation at 95℃ for 5 min, 35 cycles of denaturation at 95℃ for 20 s, annealing at 55℃ for 20 s, and extension at 70℃ for 20 s.
- changes and water temperature rise. The primers for QPCR were designed based on known sequences of black porgy GR: GR forward primer (5'・CGT TGA TGC AGC CAG GAC AGG-37), GR reverse primer (5Z-GGA ACT GCT GCT GAA CCC TCT G・3‘), p-actin forward primer (5Z-TCG AGC ACG GTA TTG TGA CC-3Z), and P-actin reverse primer (5Z-ACG GAA CCT CTC ATT GCC GA-3'). PCR amplification was performed using an iCycler iQ Multicolor Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and iQ™ SYBR Green Supermix (Bio-Rad) according to the manufacturer's instructions.
- a TRIzol kit (Gibco/BRL). Using 2.5 Jig of total RNA as a template, 3Z-RACE cDNA and 5Z-RACE cDNA were synthesized using a CapFishing™ full-length cDNA Premix Kit (Seegene, Seoul, Korea). First-strand cDNA synthesis was conducted using an oligo-d (T)i8 anchor primer (5Z-CTG TGA ATG CTG CGA CTA CGA T(T)i8-3') and CapFishing™ adaptor (Seegene).
대상 데이터
- The primers used for GR amplification were designed using highly conserved regions of gilthead seabream (Sparus aurata, GenBank accession no. DQ486890) and European seabass (Dicentrarchus labrax, AY619996): GR forward primer (5Z-CTG TTT CTC ATG TCT TTC GG-3') and GR reverse primer (5Z-TTT CGG TAA TTG GTT GCT GAT GAT-3, ). Total RNA was extracted from the black porgy kidney using a TRIzol kit (Gibco/BRL, Grand Island, NY).
- Comparison of the amino acid sequence of black porgy GR, gilthead sea bream (gsGR) GR, European sea bass (esGR) GR, and African cichlid (acGR) GR optimally aligned to match identical residues indicated by the shaded boxes. The sequences were taken from the GenBank/EMBL/DDBJ sequence databases, The GR amino acid sequences used for alignment are black porgy GR (bpGR, GenBank accession no. A AX 18925), gilthead sea bream GR (gsGR, ABF30967), European sea bass GR (esGR, AAT-41627), and African cichlid GR(acGR, AAM27887). The DNA-binding domain is indicated by an asterisk and the hormone-binding domain is boxed.
- The study was performed with 1-year-old black porgy (R=60, 14.3±0.4cm, 51.0±6.0g) reared in 220-L circulating filter tanks in the laboratory. Before the experiment, water temperature and photoperiod were 20± 1℃ and 12L:12D, respectively.
데이터처리
- , Chicago, IL). Differences in the data were compared by one-way ANOVA followed by post hoc multiple comparison test (Tukey's test).
※ AI-Helper는 부적절한 답변을 할 수 있습니다.