A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyse...
A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.
A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum sativum, Cucurbita moschata, Datura stramonium and Tetragonia expansa plants. Full-length genome sequences of CMV-Rb RNAs 1, 2 and 3 were obtained using 12 primer pairs by RT-PCR analysis. The genome of CMV-Rb RNA segments 1, 2, and 3 consists of 3363nt, 3049nt, and 2214nt in length, respectively. In order to ascertain their taxonomic identity, nucleotide and the deduced amino acid sequence analyses RNAs 1, 2 and 3 of CMV-Rb isolates were conducted with previously reported sequences of CMV strains and/or isolates. CMV-Rb RNAs showed about 90 to 99% sequence identity to those of subgroup I strains suggesting that CMV-Rb is more closely related to CMV isolates belong to subgroup I. To our knowledge, this is the first report of CMV on Rudbeckia bicolor in Korea.
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제안 방법
4 full optimal sequence alignments. Phylogenetic tree analysis was carried out using neighbor-joining method based on Jukes-Cantor genetic distances with 1000 bootstrap (Geneious 4.5.4) replications. Complete genome sequences of 13 CMV strains in GenBank were used as references and ER strain of PSV was used as an outgroup.
Sequence data was analyzed and compared with those of known CMV strain by the BLAST program at NCBI website and the multiple sequence alignment application of DNAMAN version 4.0 (Lynnon Biosoft, Germany) and Geneious v4.5.4 full optimal sequence alignments. Phylogenetic tree analysis was carried out using neighbor-joining method based on Jukes-Cantor genetic distances with 1000 bootstrap (Geneious 4.
대상 데이터
tabacum leaves by easy-spinTM Total RNA kit (iNtRON, Korea). Specific primers were designed based upon fulllength sequences of the previously reported CMV sequences available in GenBank of National Center for Biotechnology (NCBI). Reverse transcription (RT) reaction was carried out at 42℃ for 30 min, and was denatured by heating at 95℃ for 5 min in 5x RT buffer containing 5l of total RNA, 10 pmoles of the downstream primer, 2.
성능/효과
This suggests that the subgroups I strains are evolving more rapidly, and three genomic RNAs of CMV has a different evolution history. Results of this study supported the idea that differences in symptomatology observed from different host plants may be at least partly due to the differences between CMV-Rb RNA 1 belonging to subgroup IB and CMV-Rb RNAs 2 and 3 belonging to subgroup IA. A further study is, however, needed like construction of infectious clones to clarify these relations and to characterize the host factors required for CMV infections.
후속연구
Results of this study supported the idea that differences in symptomatology observed from different host plants may be at least partly due to the differences between CMV-Rb RNA 1 belonging to subgroup IB and CMV-Rb RNAs 2 and 3 belonging to subgroup IA. A further study is, however, needed like construction of infectious clones to clarify these relations and to characterize the host factors required for CMV infections. Altogether, results of this study indicated that the virus isolate causing symptoms of yellow mosaic, fern leaves, malformation, and necrosis on R.
참고문헌 (24)
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