[논문]황색 포도상구균과 대장균에 대한 은나노 입자의 항균활성

김수환; 이형선; 류덕선; 최수재; 이동석

황색 포도상구균과 대장균에 대한 은나노 입자의 항균활성
Antibacterial Activity of Silver-nanoparticles Against Staphylococcus aureus and Escherichia coli 원문보기

한국미생물·생명공학회지 = Korean journal of microbiology and biotechnology, v.39 no.1, 2011년, pp.77 - 85

김수환 (인제대학교 식의약생명공학과) , 이형선 (인제대학교 식의약생명공학과) , 류덕선 (인제대학교 식의약생명공학과) , 최수재 (인제대학교 식의약생명공학과) , 이동석 (인제대학교 식의약생명공학과)

초록
AI-Helper

본 연구는 은나노 입자의 항균활성을 알아보기 위하여, 그람 양성세균인 황색포도상구균과 그람 음성세균인 대장균에 대한 은나노 입자(Ag-NPs)를 처리 후, 세균세포 생장곡선측정, 활성산소생성능 측정, 세포질 단백질 누출량 측정, 젖산탈수소효소 활성측정 및 고분해능 임계방사 주사전자현미경 관찰이 수행되었다. 세균세포의 생장곡선 측정은 다양한 농도, 배양시간, 배양온도 및 pH에서 수행되었다. 결과적으로 황색 포도상구균과 대장균은 배양온도와 pH에 영향을 받지않고 은나노 입자에 의해 효과적으로 생장억제가 이루어지는 것을 관찰할 수 있었다. 또한 활성산소의 생성에 의하여 세포막의 파괴로 세포질내 물질의 유출을 세포질 유래 단백질 측정으로 확인할 수 있었으며, 젖산탈수소효소 활성측정을 통하여 은나노 입자에 대한 세포호흡억제활성 또한 확인할 수 있었다. 임계방사 주사전자현미경 관찰결과 은나노 입자에 의한 세균 세포표면의 형태학적 변화 또한 관찰되었다. 이러한 결과를 통하여 은나노 입자를 효과적인 항균활성소재로 활용 가능함이 입증되었다.

Abstract ▼ AI-Helper

The antibacterial activities of silver nanoparticles (Ag-NPs) were studied with respect to Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli by observing the bacterial cells treated or not with Ag-NPs by FE-SEM as well as measuring the growth curves, formation of bactericidal ROS, protein leakage, and lactate dehydrogenase activity involved in the respiratory chain. Bacterial cells were treated with Ag-NPs powder, and the growth rates were investigated under varying concentrations of Ag-NPs, incubation times, incubation temperatures, and pHs. As a result, S. aureus and E. coli were shown to be substantially inhibited by Ag-NPs, and the antibacterial activity of Ag-NPs did not fluctuate with temperature or pH. These results suggest that Ag-NPs could be used as an effective antibacterial material.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

문제 정의

Furthermore, the morphological changes on bacterial cells by Ag-NPs were observed by FE-SEM. This study indicates that Ag-NPs can be used as effective antibacterial materials against various microorganisms which can endanger human beings.

제안 방법

Oxidative stress can not only cause damage to the cell membrane, but can also cause damage to the proteins, DNA, and intracellular systems such as the respiratory system. In this study, ROS was measured using DCFDA [Fig. 3]. After 3 h incubation, significantly increased ROS was detected in Ag-NPs treated group of S.

대상 데이터

The S. aureus and E. coli cells used in the present study were supplied by Busan Paik hospital, Inje university (Busan, Korea).
The silver nanoparticles (Ag-NPs) powder used in this study was manufactured by Thermolon Korea, Inc. (Busan, Korea). The powder containing 5% silver was silver-brown and insoluble.

데이터처리

All experimental data were compared using Student’s t-test.

이론/모형

Protein leakage from bacterial cells was detected using Bradford’s protein assay [3].
The minimum inhibitory concentration (MIC) of Ag-NPs was determined using the plate count method [18, 30]. The Ag-NPs powder was sterilized with UV radiation for 1 h, and the weight of the powder was then measured under aseptic conditions.

성능/효과

In conclusion, this study showed that Ag-NPs have potent antibacterial activities against S. aureus and E. coli cells. The growth and reproduction of Ag-NPs treated bacterial cells were quickly inhibited.

참고문헌 (32)

Amro, N. A., L. P. Kotra, K. Wadu-Mesthrige, A. Bulychev, S. Mobashery, and G. Liu. 2000. High-resolution atomic force microscopy studies of the Escherichia coli outer membrane: structural basis for permeability. Langmuir 16: 2789- 2796.

상세보기
Berger, T. J., J. A. Spadaro, S. E. Chapin, and R. O. Becker. 1996. Electrically generated silver ions: quantitative effects on bacterial and mammalian cells. Antimicrob. Agents Ch. 9: 357-358.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248- 254.
Brown, T. and D. Smith. 1976. The effects of silver nitrate on the growth and ultrastructure of the yeast Cryptococcus albidus. Microbios Lett. 3: 155-162.
Corinne, Pellieux, A. Dewilde, C. Pierlot, and J.-M. Aubry. 2000. Bactericidal and virucidal activities of singlet oxygen generated by thermolysis of naphthalene endoperoxides. Methods in Enzymology. 319: 197-207.

상세보기
Danilczuk, M., A. Lund, J. Saldo, H. Yamada, and J. Michalik. 2006. Conduction electron spin resonance of small silver particles. Spectrochim. Acta. Part A: Mol. Biomol. Spectrosc. 63: 189-191.
Feng, Q. L., J. Wu, G. Q. Chen, F. Z. Cui, T. N. Kim, and J. O. Kim. 2000. A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. J. Biomed. Mater. Res. 52: 662-668.
Fuhrmann, G. F. and A. Rothstein. 1968. The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim. Biophys. Acta. 163: 331-338.
Garrard, W. and J. Lascelles. 1968. Regulation of Staphylococcus aureus lactate dehydrogenase. J. Bacteriol. 95: 152- 156.
Guerlava, P., V. Izac, and J.-L. Tholozan. 1998. Comparison of different methods of cell lysis and protein measurements in clostridium perfringens: Application to the cell volume determination. Curr. Microbiol. 36: 131-135.
Izatt, R. M., C. J. J., and J. H. Rytting. 1971. Sites and thermodynamic quantities associated with proton and metal ion interaction with ribonucleic acid, deoxyribonucleic acid, and their constituent bases, nucleosides, and nucleotides. Chem. Rev. 71: 439-481.

상세보기
Jones, S. A., P. G. Bowler, M. Walker, and D. Parsons. 2004. Controlling wound bioburden with a novel silver-containing Hydrofiber dressing. Wound Repair Regen. 12: 288-294.

상세보기
Jung, W. K., H. C. Koo, K. W. Kim, S. Shin, S. H. Kim, and Y. H. Park. 2008. Antibacterial activity and mechanism of action of the silver ion in Staphylococcus aureus and Escherichia coli. Am. Soc. Microbiol. 74: 2171-2178.
Kim, J. S., E. Kuk, K. N. Yu, J.-H. Kim, S. J. Park, H. J. Lee, S. H. Kim, Y. K. Park, Y. H. Park, et al. 2007. Antimicrobial effects of silver nanoparticles. Nanomed-Nanotechnol. 3: 95- 101.
Kim, J. Y., K. Sungeun, J. Kim, L. Jongchan, and J. Yoon. 2005. The biocidal activity of nano-sized silver particles comparing with silver ion. Korean Soc. Environ. Eng. 27: 771-776.
Kye, I.-S., Y.-S. Jeon, J.-K. No, Y.-J. Kim, K. H. Lee, K. H. Shin, J. Kim, T. Yokozawa, and H.-Y. Chung. 1999. Reactive oxygen scavenging activity of green tea polyphenols. J. Korea Gerontol. 9: 10-17.
Li, W. R., X. B. Xie, Q. S. Shi, H.-Y. Zeng, Y.-S. OU-Yang, and Y.-B. Chen. 2009. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli. Microb. Cell Physiol. 85: 1115-1122.
Magana, S. M., P. Quintana, D. H. Aguilar, J. A. Toledo, C. Angeles-Chavez, M. A. Cortes, L. Leon, Y. Freile-Pelegrin, and T. Lopez, et al. 2008. Antibacterial activity of montmorillonites modified with silver. J. Mol. Catal. A: Chem. 281: 192-199.

상세보기
Miller, L. P. and S. E. A. McCallan. 1957. Toxic action of metal ions to fungus spores. J. Agric. Food Chem. 5: 116- 122.
Pinto, R. J. B., P. A. A. P. Marques, C. P. Neto, T. Trindade, S. Daina, and P. Sadocco. 2009. Antibacterial activity of nanocomposites of silver and bacterial or vegetable cellulosic fibers. Acta Biomater. 5: 2279-2289.

상세보기
Rahn, R. O. and L. C. Landry. 1973. Ultraviolet irradiation of nucleic acids complexed with heavy stoms. II. Phosphorescence and photodimerization of DNA complexed with Ag. Photochem. Photobiol. 18: 29-38.

상세보기
Rayman, M. K., T. C. Lo, and B. D. Sanwal. 1972. Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. J. Biol. Chem. 247: 6332-6339.
Richards, R. M. E., H. A. Odelola, and B. Anderson. 1984. Effect of silver on whole cells and spheroplasts of a silver resistant Pseudomonas aeruginosa. Microbios. 39: 151-157.

상세보기
Schreurs, W. J. and H. Rosenberg. 1982. Effect of silver ions on transport and retention of phosphate by Escherichia coli. J. Bacteriol. 152: 7-13.
Shahverdi, A. R., A. Fakhimi, H. R. Shahverdi, and S. Minaian. 2007. Synthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli. Nanomed-Nanotechnol. 3: 168-171.
Silva Paula, M. M. d., C. V. Franco, B. M. Cesar, L. Rodrigues, T. Barichello, G. D. Savi, L. F. Bellato, M. A. Fiori, and L. d. Silva. 2009. Synthesis, characterization and antibacterial activity studies of poly-{styrene-acrylic acid} with silver nanoparticles. Mater. Sci. Eng. 29: 647-650.
Sondi, I. and B. Salopel-sondi. 2004. Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria. J. Colloid Interface Sci. 275: 177- 182.

상세보기
Stockland, A. E. and C. L. San clemente. 1968. Lactate dehydrogenase activity in certain strains of Staphylococcus aureus. J. Bacteriol. 95: 74-80.
Thomas J., Silhavy, D. Kahne, and S. Walker. 2010. The bacterial Cell Envelope. Cold Spring Harb Perspect Biol. 2: a000414.
Wang, J.-X., L.-X. Wen, Z.-H. Wang, and J.-F. Chen. 2006. Immobilization of silver on hollow silica nanospheres and nanotubes and their antibacterial effects. Mater. Chem. Phys. 96: 90-97.

상세보기
Yakabe, Y., T. Sano, H. Ushio, and T. Yasunaga. 1980. Kinetic studies of the interaction between silver ion and deoxyribonucleic acid. Chem. Lett. 4: 373-376.
Zavriev, S. K., L. E. Minchenkova, M. Vorlickova, A. M. Kolchinsky, M. V. Volkenstein, and V. I. Ivanov. 1979. Circular dichroism anisotropy of DNA with different modifications at N7 of guanine. Biochim. Biophys. Acta. 564: 212- 224.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증