[논문]감초 약침액이 대식세포주에서 항염증효과에 미치는 영향

박종필; 손정현; 김용민; 이은용; 임강현; 김이화

감초 약침액이 대식세포주에서 항염증효과에 미치는 영향
Suppression of Inflammatory Macrophage Responese by Glycyrrhiza Uralensis Herbal Acupuncture Extract 원문보기

경락경혈학회지= Korean journal of acupuncture, v.28 no.4, 2011년, pp.49 - 58

박종필 (세명대학교 한방바이오산업 임상지원센터) , 손정현 (세명대학교 한방바이오산업 임상지원센터) , 김용민 (세명대학교 한방바이오산업 임상지원센터) , 이은용 (세명대학교 한의과대학 침구학교실) , 임강현 (세명대학교 한의과대학 본초학교실) , 김이화 (세명대학교 한의과대학 경락경혈학교실)

초록
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목적 : 본 연구는 감초의 염증에 대한 효과를 연구하였다. 감초의 에탄올 그리고 물로 추출한 두 가지의 약침액을 이용하여 쥐의 대식세포에서 유래한 RAW264.7 세포에 대한 염증 억제효과를 확인하였다. 방법 : Inducible nitric oxide synthase(iNOS), cyclooxygenase-2(COX-2)를 포함한 염증성 단백질의 발현과 extracellular signal-regulated kinase 1/2(ERK1/2) 그리고 phosphorylated ERK1/2 의 발현을 Western blot 으로 확인하였고, PGE2의 발현은 ELISA 로 확인하였다. 결과 : RAW264.7 세포에 감초의 물 혹은 에탄올 추출 약침액을 투여한 결과 투여된 농도에 따라 LPS로 유도된 NO의 생성이 억제되었으며 iNOS, COX-2, 그리고 인산화 ERK1/2 의 발현도 감소되었다. 결론 : 본 실험 결과, 적작약의 물 그리고 에탄올 추출 약침액에 대하여 항염증성 효과가 있음을 확인하였다.

주제어

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가설 설정

7 cells were treated with 1, 5, 25, 125, 625, 3125 ㎍/㎖ of ethanol extract for 16 h and cell toxicity was observed at a concentration of 3125 ㎍/㎖, as determined by MTT assay. Next, we asked whether the ethanol extract of G. uralensis has antiinflammatory properties as seen with the water extract. We observed a decrease in NO production starting at 5 ㎍/㎖ of extract (Fig.

제안 방법

In this study, we prepared two types of extracts from G. uralensis, using a water-based or ethanol-based extraction method. We evaluated the effect of each extract for anti-inflammatory activity and cytotoxicity.

이론/모형

0 × 104 cells/well) were stimulated with LPS (1 ㎍/ml) and extract. Cell viability was determined using the MTT method. Values are presented as the mean ± S.
0 × 105 cells/well) were stimulated with 1㎍/㎖ of LPS or with LPS plus various concentrations (1, 5, 25, 125, 625, 3125 ㎍/㎖) of ethanol-based or water-based Glycyrrhiza uralensis extract for 24 h. Nitric oxide production was determined using the Griess reagent method. Cell viability was determined 24 h after the cells (1.

성능/효과

Suppression of PGE2 production relieves inflammation and pain. Our results show that LPS-stimulated PGE2 was suppressed by G. uralensis extracts in RAW264.7 cells; however, the decrease was slight. Importantly, this inhibition was concentrationdependent and did not exhibit any cell-toxicity.
uralensis extract correlated with a decrease in NO release. These results suggest that the water-based extract prepared from G. uralensis has anti-inflammatory effects because of the decrease in NO production even at concentrations as low as 125 ㎍/㎖.

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