[논문]꿀풀 에탄올 추출물 및 분획물의 항산화성

박동식; 박미영; 전상민; 이진영; 이영민; 장환희; 황경아; 김재현

doi:10.7783/kjmcs.2011.19.6.484

꿀풀 에탄올 추출물 및 분획물의 항산화성
Antioxidant Activity of Different Solvent Fractions from Prunella vulgaris var. lilacina 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.19 no.6, 2011년, pp.484 - 490

박동식 (농촌진흥청 국립농업과학원 농식품자원부) , 박미영 (농촌진흥청 국립농업과학원 농식품자원부) , 전상민 (농촌진흥청 국립농업과학원 농식품자원부) , 이진영 (농촌진흥청 국립농업과학원 농식품자원부) , 이영민 (농촌진흥청 국립농업과학원 농식품자원부) , 장환희 (농촌진흥청 국립농업과학원 농식품자원부) , 황경아 (농촌진흥청 국립농업과학원 농식품자원부) , 김재현 (농촌진흥청 국립농업과학원 농식품자원부)

Abstract ▼ AI-Helper

The potential antioxidant activities of different fractions from Prunella vulgaris var. lilacina were assayed in vitro. Among several fractions, n-BuOH fraction showed the highest 1,1-di[henyl-2-picrylhydrazyl (DPPH) free radical scavenging ($IC_{50}=0.50{\mu}g/mL$). The results of 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging activity and ferric reducing antioxidant power (FRAP) assay showed the concentration dependency and n-BuOH fraction appeared a better result than the other fractions at the same concentrati on in this study. Moreover the total phenol and flavonoid contents of n-BuOH fraction contained the highest level. Additionally, correlation analysis indicated a high correlation between the antiradical activity and the total phenolic and flavonoid contents (p < 0.001). It suggests that n-BuOH fraction obtained from the 70% EtOH crude extract of Prunella vulgaris var. lilacina has wide potential for use as a source of antioxidant material.

주제어

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데이터처리

Correlations among data obtained were calculated using Pearson’s correlation coefficient (R).
Data were expressed as means ± S.D. Data were analyzed by one-way analysis of variance (ANOVA) followed by Duncan’s multiple range tests.

이론/모형

The FRAP assay was performed according to the modified Benzie and Strain method (Benzie and Strain, 1996). The stock solutions included 300 mM acetate buffer (3.
The total flavonoids were detrmined using the method of Jia et al. (1999). A volume of 0.
Total phenols were determined by using the FolinCiocalteu reagent according to the method of Ainsworth and Gillespie (Ainsworth and Gillespie, 2007). Briefly, a 50 µL of various fractions was assayed with 250 µL Folin reagent and 500 µL of sodium carbonate (20%, w/v).

성능/효과

, 1996; Bors and Michel, 2002). In our results, positive correlations were found between ABTS and FRAP assays and total phenolic and flavonoid contents. These results indicated that a relationship between phenolic compound concentration in various fractions from 70% EtOH crude extract of Prunella vulgaris var.
In this study, Vit C and α-tocopherol were measured as the positive controls, showing the IC50 values of 0.08 and 0.98 ㎎/mL, respectively (Table 2).
There were positively high correlation between phenol contents and ABTS assay (R = 0.901, P < 0.001), phenol contents and FRAP assay (R = 0.916, P < 0.001), and flavonoid contents and ABTS assay (R = 0.826, P < 0.001).

후속연구

, 1999). Future studies should focus on the identification of these antioxidants and purification of this plant ingredient into better agents with high efficacy and activity.

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