선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- (Shizuoka, Japan) and housed under standard conditions (12-h light/dark cycle, 22℃). Animals were divided into 3 groups (10 individuals/group) - a control group, a CFE group, and a positive control group using the popular agent, orlistat (Roche, SF, USA). All 3 mouse groups were fed on high fat diet (45% kcal from fat, D12451 Research Diets, NJ, USA) for the initial 2 weeks of the experiment upon arrival.
- Anti-angiogenesis experiment of 18 natural products with the positive control, EGCG. HUVEC was cultured on Matrigel-coated wells, and the tube formation of HUVEC cells was randomly photographed using a digital camera, and analyzed by the Scion Image software.
- In order to evaluate the differentiation responses of translational factors before and after differentiation, 3T3-L1 preadipocytes were treated with MDI and different concentrations (1, 5, and 10 mg/1) of CFE. Proteins were isolated from 3T3-L1 preadipocytes and western blot analysis was conducted.
- 3333px;">USA) as a positive control, and cultured for 4 hours in a 5% CO2 incubator at 37℃. The tube formation of HUVEC was photographed using 찼 digital camera (Nikon, Tokyo, Japan), and the tube length was analyzed using Scion Image software (NIH, ML, USA). The tube lengths were compared to those of EGCG, and a candidate from the C.
대상 데이터
- 18 natural products were purchased from a Korean herbal medicine dealer in Yosu, Chonnam, Korea. The pn)ducts were homogenized with ethanol at a ratio of 4 : 6 (w/v).
- 4-week-old male C57BL/6J-ob/ob mice were obtained from SLC, Inc. (Shizuoka, Japan) and housed under standard conditions (12-h light/dark cycle, 22℃). Animals were divided into 3 groups (10 individuals/group) - a control group, a CFE group, and a positive control group using the popular agent, orlistat (Roche, SF, USA).
- The tube formation of HUVEC was photographed using 찼 digital camera (Nikon, Tokyo, Japan), and the tube length was analyzed using Scion Image software (NIH, ML, USA). The tube lengths were compared to those of EGCG, and a candidate from the C. fructus extracts (CFE) was selected for this study, among 18 natural extracts.
- The cells w샩薑샨 treated overnight with different dos샨$ of CFE. U937 (2.5X105) was added to the HUVEC cells, incubated for 30 minutes at 37℃, washed 3 times with 1% FBS/PBS, and then the average cell numbers were 야d on 5 randomly photographed ar냐as.
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