선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- The same light was used in the designated dark room and the picture angle was preset with stanchion to keep the same conditions before and after the trial. Also, the subjects were evaluated by asking the question on the level of improvement in the acne skin at the time of trial completion.
- The clinical trial was conducted to evaluate the efficacy of the composite mixture as the above on the acne facial skin by the following methods. First, 17 male and female adults in between 20 and 40s who had complained the trouble due to the acne or have the lesion of acne were selected among the volunteers and they applied the cosmetic soap to their faces and washed it twice a day, once in the morning and once in the evening, for 2 months. The pictures of the skin status before and after the application were compared after using the cosmetic soap for 2 months.
- The solid type samples were prepared by the mixture of cosmetic soap with Carthamustinctorius (safflower) flower extract as a main ingredient and various aroma essential oils (evening primrose oil, calendula oil, German chamomile oil, lavender oil, and tea tree oil). The clinical trial was conducted to evaluate the efficacy of the composite mixture as the above on the acne facial skin by the following methods. First, 17 male and female adults in between 20 and 40s who had complained the trouble due to the acne or have the lesion of acne were selected among the volunteers and they applied the cosmetic soap to their faces and washed it twice a day, once in the morning and once in the evening, for 2 months.
- Ajou University), HaCaT (KCLB 3002, Korea Cell Line Bank), FBS (Gibco, CA), RPMI1640 (WelGENE, Korea), Penicillin/Streptomycin (WelGENE, Korea), PBS (Gibco, CA), MTT (3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma, MI), Dimetyl sulfoxide (Duchefa biochemicls, Nederland), etc as the main materials. The experimental theory is to understand cell viability utilizing HaCaT cell culture in order to test the cytotoxicity of the test material after processing with it and culturing the cell. MTT assay is used to measure the viable cells.
- The test material was labeled as IUK (safflower extract). The test is to determine the existence of antimicrobial materials and their concentrations in the solution with the following methods; smearing the antimicrobial solution with certain concentration on each disc, putting on the solid agar media smeared by test germ, culturing them, and measuring the size of transparent zone (growth inhibition halo) that has no germs near the disc. The transparent zone means that the antimicrobial material inhibits the growth of germs by diffusion into the media.
- The transparent zone means that the antimicrobial material inhibits the growth of germs by diffusion into the media. The test procedures are pre-culture of test germ in the liquid media for 1-2 days, followed by adding pre-culture solution to 7 ml of 0.8% soft agar media so as to be 105,6cells/ml of germ concentration, and inoculation of agar media with germ on the test media. Then, dampen 50 µl erythromycin sample, which is the positive control, on each paper disc and drop this on the media.
대상 데이터
- The pictures of the skin status before and after the application were compared after using the cosmetic soap for 2 months. DSLR camera (NS10, Samsung, Korea) was used in the clinical trial. The same light was used in the designated dark room and the picture angle was preset with stanchion to keep the same conditions before and after the trial.
- DDT test was performed in vitro after the dissolution of lyophilized powder into the solvent. The main equipments and test materials for DDT test of safflower extract were as follows; Shaking incubator (SI 600R, Jeiotech, Korea), CO2 Incubator (IB-600M, Jeiotech, Korea), Clean bench, and Autoclave (S120V, Tomy, Korea) as the main equipments, and Propionibacteriumacnes (ATCC6919), etc as the main materials. The test material was labeled as IUK (safflower extract).
- After the lyophilized powder was dissolved in the solvent, cell viability test was performed in vitro to be categorized by concentration. The main equipments and test materials for the cell viability test of safflower extract were as follows; ELISA Reader system (Powerwave X, Bio-tek inc, Japan), Clean bench (SH-120S., Hansol SM, KR), Inverted-microscope (TS-100F, Nikon, JPN), etc as the main equipments, and human normal fibroblast (from neonatal foreskin, Dermatology Dept. Ajou University), HaCaT (KCLB 3002, Korea Cell Line Bank), FBS (Gibco, CA), RPMI1640 (WelGENE, Korea), Penicillin/Streptomycin (WelGENE, Korea), PBS (Gibco, CA), MTT (3- (4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (Sigma, MI), Dimetyl sulfoxide (Duchefa biochemicls, Nederland), etc as the main materials. The experimental theory is to understand cell viability utilizing HaCaT cell culture in order to test the cytotoxicity of the test material after processing with it and culturing the cell.
- The main equipments and test materials for DDT test of safflower extract were as follows; Shaking incubator (SI 600R, Jeiotech, Korea), CO2 Incubator (IB-600M, Jeiotech, Korea), Clean bench, and Autoclave (S120V, Tomy, Korea) as the main equipments, and Propionibacteriumacnes (ATCC6919), etc as the main materials. The test material was labeled as IUK (safflower extract). The test is to determine the existence of antimicrobial materials and their concentrations in the solution with the following methods; smearing the antimicrobial solution with certain concentration on each disc, putting on the solid agar media smeared by test germ, culturing them, and measuring the size of transparent zone (growth inhibition halo) that has no germs near the disc.
이론/모형
- 2. Pictures of antimicrobial effect distribution by DDT (Disk Diffusion Test) assay.
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