[논문]택사(澤瀉) 약침액(藥鍼液)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향

이세나; 김명규; 김명희; 김형준; 조학준; 김이화; 임강현

[국내논문] 택사(澤瀉) 약침액(藥鍼液)의 elastase 활성과 DPPH, NO 소거능에 미치는 영향
Effects of Alismatis Rhizoma Pharmacopuncture Extracts on the Elastase Activity and DPPH and NO Scavenging Activities 원문보기

경락경혈학회지= Korean journal of acupuncture, v.28 no.1, 2011년, pp.15 - 22

이세나 (세명대학교 한의과대학) , 김명규 (세명대학교 한의과대학) , 김명희 (세명대학교 간호학과) , 김형준 (세명대학교 한의과대학) , 조학준 (세명대학교 한의과대학) , 김이화 (세명대학교 한의과대학) , 임강현 (세명대학교 한의과대학)

초록
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엘라스타제는 폐의 탄성 섬유를 파괴하여 폐기종과 폐고혈압을 유발하는 역할을 한다. 이에 본 연구는 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제 억제효능과 항산화효능을 측정하여 폐기종과 폐고혈압 치료의 가능성을 확인하고자 실시 하였다. 방법 : 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제에 대한 억제효능과 di(phenyl)-(2,4,6-trinitrophenyl) iminoazanium (DPPH) 및 nitric oxide (NO) 자유기 소거능을 측정하였다. 결과 : 택사(澤瀉) 약침액(藥鍼液)은 엘라스타제 활성을 통계적으로 유의하게 억제하였고, DPPH 및 NO 자유기 소거능이 있음이 통계적으로 유의하게 확인되었다. 결론 : 본 연구를 통해 택사(澤瀉) 약침액(藥鍼液)의 엘라스타제 억제효능과 항산화 효능이 확인되었다. 본 연구 결과를 근거로 시험관내 실험이외의 추가적인 연구를 통해 폐기종과 폐고혈압 치료제로서 택사(澤瀉) 약침액(藥鍼液)의 활용을 고려해 볼 수 있을 것으로 기대한다.

주제어

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대상 데이터

Alismatis Rhizoma (the root of Alisma orientalis) was purchased from Omniherb (Korea). ARPE was prepared as follow.

데이터처리

Significances of changes were determined using the one-way ANOVA with a Dunnett's post hoc test.

이론/모형

The elastase activity was evaluated by using a modification of a previously reported method of Kraunsoe et al¹⁶⁾. In order to evaluate the inhibition of elastase activity, the amount of released p-nitroaniline, which was hydrolyzed from the substrate, N-succinyl-Ala-Ala-Ala-p-nitroanilide, by elastase, was read with a maximum absorbance at 410 nm17).
It has been reported that reactive free radicals was induced by elastase in neutrophil and that they play a role in inflammation. Assays of the free radical scavenging capacity were carried out by the DPPH method. The free radical scavenging capacity of sample was measured at each concentration (0, 4, 20, 100, and 500 mg/ml).

성능/효과

ARPE was found to inhibit elastase activity significantly at all concentrations of ARPE 10 mg/ml, 1 mg/ml, and ARPE 0.1 mg/ml treated groups (21.5 ± 2.4%, 38.0 ± 2.8%, and 51.3 ± 0.7% compared with 100% elastase activity of control group respectively, Fig. 1).
ARPE 50 mg/ml treated groups had the highest scavenging capability, of 102.8 ± 0.1%, while 10, 2, and 0.4 mg/ml treated groups had 57.8 ± 0.1%, 14.8 ± 0.1%, and 3.3 ± 0.0%, respectively(Fig. 3).
ARPE 10 mg/ml treated groups had the highest scavenging capability, of 92.3 ± 0.0%, while 50, 2, and 0.4 mg/ml treated groups had 51.4 ± 0.2%, 20.9 ± 0.1%, and 11.5 ± 0.3%, respectively(Fig. 4).
In conclusion, ARPE showed the inhibiting effects on the elastase, and free radical scavenging capability of DPPH and nitrite. These results suggest that ARPE may have potential effects for pulmonary emphysema and pulmonary hypertension.

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