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[국내논문] Collagenase-induced Arthritis Rat Model에서 Thermal Hyperalgesia에 대한 전침(電鍼)의 진통효과(鎭痛效果) 및 기전연구: Adrenergic Mechanism에 대(對)한 연구(硏究)
The Analgesic Effect and the Mechanism of Electroacupuncture on Thermal Hyperalgesia in the Rat Model of Collagenase-induced Arthritis: Mediation by Adrenergic Receptors 원문보기

大韓鍼灸學會誌= The journal of Korean Acupuncture & Moxibustion Society, v.28 no.2, 2011년, pp.57 - 67  

서병관 (경희대학교 한의과대학 침구학교실) ,  박동석 (경희대학교 한의과대학 침구학교실) ,  백용현 (경희대학교 한의과대학 침구학교실)

초록
AI-Helper 아이콘AI-Helper

목적 : Collagenase-induced osteoarthritis(OA) 동물 모델에서 전침의 adrenergic mechanism을 연구하고자 한다. 방법 : Collagenase-induced arthritis(CIA)를 유발하기 위하여 5주령의 male Sprague-Dawley rat의 뒷다리 좌측 무릎 관절에 0.05ml의 4mg/ml collagenase solution을 intra-articular 주입하고, 다시 4일 후에 같은 부위에 같은 농도의 collagenase solution을 intra-articular boosting injection 시행한 뒤, gross, histopathological features 및 biomarker activity 변화를 관찰하였다. 예비실험을 통하여 CIA rat model에서 진통효과를 발휘하는 것으로 확인한, 족삼리(足三里) ($ST_{36}$)에 대한 저빈도 train pulse EA stimulation (2Hz, 0.07 mA, 0.3ms)을 침치료 방법으로 적용하였다. 전침의 진통기전을 확인하기 위하여, ${\alpha}1$-adrenergic antagonist (prazosin, 1 mg/kg, i.p.), ${\alpha}2$-adrenergic receptor antagonist (yohimbine, 2mg/kg, i.p.), ${\alpha}1$-adrenergic receptor agonist(phenylephrine, 2mg/kg, i.p.), ${\alpha}2$-adrenergic receptor agonist(clonidine, $40{\mu}g$/kg, i.p.)을 전침시행 20분 전에 복강 내로 전처치하였다. Tail flick unit(Ugo Basile Model 7360)을 이용하여 열자극에 대한 통증역치를 측정하였다. 결과 : 퇴행성관절염 징후(gross, histopathological features)와 통증역치의 변화가 최대값을 나타내는 CIA 유발 4주차에 저빈도 전침자극(train pulse, 2Hz, 0.07mA, 0.3ms)을 족삼리($ST_{36}$)에 적용하였으며, 족삼리 전침의 진통효과는 ${\alpha}2$-adrenergic receptor antagonist(yohimbine, 2mg/kg, i.p.)전처치에 의해 억제되었으나, ${\alpha}1$-adrenergic antagonist(prazosin, 1 mg/kg, i.p.)전처치에는 억제되지 않았다. 또 ${\alpha}2$-adrenergic receptor agonist(clonidine, $40{\mu}g$/kg, i.p.)의 전처치를 통하여 유의한 synergistic analgesic effect가 관찰되었으나, ${\alpha}1$-adrenergic receptor agonist(phenylephrine, 2mg/kg, i.p.)의 전처치는 전침의 진통효과에 synergistic effect를 미치지 않는 것으로 나타났다. 결론 : 저빈도 족삼리 전침은 CIA로 유발된 염증성 통증에 대하여 진통효과를 발휘하며, 이는 ${\alpha}2$-adrenergic receptor에 의하여 매개되는 것으로 보이며 ${\alpha}1$-adrenergic receptor는 영향을 미치지 않는 것으로 사료된다.

Keyword

#electroacupuncture   #collagenase-induced arthritis   #adrenergic receptor   #antinociceptive mechanism  

AI 본문요약
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* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

  • 3 × 18 cm in diameter × length) with their tails protruding outside for 60 min/day for 7 days. Adaptation was conducted before TFL assessment and EA treatment.
  • A standard frontal section of 5μm was prepared and stained with hematoxylin and eosin(H & E staining). The following six parameters were evaluated in the histological analysis: loss of the superficial layer, erosion of cartilage, fibrillation and/or fissures, disorganization of chondrocytes, loss of chondrocytes, and cluster formation. All sections were graded using a Biocom microscope with an Axio Cam camera(Carl Zeiss, Germany) by two independent observers who were blinded to the treatment groups.
  • The pain threshold to thermal stimuli was assessed at the tail of a rat restrained in a plastic holder by the tail flick unit(Ugo Basile Model 7360, Comrio, Italy), according to D’Amour and Smith, 1941. The mean reaction time was assessed as the time lapse between the initiation of irradiation of infrared bulb of tail flick unit and the evasion of tail from three consecutive measurements at baseline, 10, 20, 30, 45 and 60 minutes. Preliminary exploratory experiments set the intensity as basal latency of 10 seconds in normal rats and the maximum cut-off latency as 20 seconds to avoid thermal injury.

대상 데이터

  • The following six parameters were evaluated in the histological analysis: loss of the superficial layer, erosion of cartilage, fibrillation and/or fissures, disorganization of chondrocytes, loss of chondrocytes, and cluster formation. All sections were graded using a Biocom microscope with an Axio Cam camera(Carl Zeiss, Germany) by two independent observers who were blinded to the treatment groups.
  • Five-week-old male Sprague Dawley rats(Samtaco, Osan, Korea, 200g) were housed under specific pathogen-free conditions under controlled temperature(22 ± 1℃), humidity(55 ± 5%), and a 12:12 light-dark cycle(light on 6:00 AM to 6:00 PM) with food and water available ad libitum.

데이터처리

  • The results are presented as the mean ± S.D. The significance of the statistical differences was determined using the non-parametric Friedman’s rank test followed by Dunnett’s post hoc test in a group, non-parametric Mann-Whitney U test between two groups, and non-parametric Kruskal-Wallis ANOVA followed by Dunnett’s post hoc test among groups.
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