선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- Linear regression analysis was performed by plotting the peak area of each standard compound on the y-axis, versus the concentration of each compound on the x-axis. The stock solutions of standard compounds were diluted to smallest concentration with methanol.
- The intra- and inter-day precisions were determined by analyzing three different concentrations of each compound (i.e., low, medium, high) spiked in sample extract three times in a day and in three successive days for inter-day. The relative standard deviation (RSD) was measured to assess precision.
- The accuracy was evaluated by recovery test. The recovery test was conducted by adding three different concentrations of each known standard (i.e., low, medium, and high) to samples before extraction. Samples were extracted and analyzed using the method described above.
- To evaluate the accuracy of our method, recovery was measured by adding three different concentrations of mixed standard solutions to samples.
- Detection was performed at maximum absorption wavelengths of each compound, 254 and 280 nm, respectively. With the PDA, UV spectra of the reference compounds in SST could be compared with those of standard compounds, therefore each reference compound was identified by comparing both the retention time and UV spectra with standard compounds. All analytes showed baseline separation and they were eluted within 40 min.
대상 데이터
- Four-week-old male Sprague-Dawley (SD) rats were purchased from OrientBio Inc. (Seongnam, Korea) and housed in controlled environmental conditions (22±3°C with 12/12 hrs light/dark cycle) for one week prior to the experiment.
- RAW 264.7 cells, murine macrophage cell line, were obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco BRL, Grand island, NY, USA) supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin (Gibco BRL) and 5.5% fetal bovine serum (FBS; Gibco BRL).
- The analysis was carried out on a Gemini C18 (250×4.6 mm, 5 μm, Phenomenex, Torrance, CA, USA).
- 1). The herbal materials of SST were purchased from Omniherb (Yeongcheon, Korea) and HMAX (Jecheon, Korea). It was taxonomically confirmed by Professor Je-Hyun Lee, Dongguk University, Gyeongju, Republic of Korea.
데이터처리
- The results were represented as means±SEM and deemed statistically significant at p<0.05, based on ANOVA or Bonferroni multiple comparison analysis using the Systat®program (Ver.8.0, SYSTAT Inc., Evanston, IL, USA).
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