[논문]한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석

카야디; 디아 마하라니; 유승희; 이승환; 이준헌

doi:10.7744/cnujas.2012.39.3.349

한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석
Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo) 원문보기

농업과학연구 = CNU Journal of agricultural science, v.39 no.3, 2012년, pp.349 - 355

카야디 (충남대학교 농업생명과학대학 동물자원생명과학과) , 디아 마하라니 (충남대학교 농업생명과학대학 동물자원생명과학과) , 유승희 (충청남도 축산기술연구소) , 이승환 (축산과학원 한우시험장) , 이준헌 (충남대학교 농업생명과학대학 동물자원생명과학과)

Abstract ▼ AI-Helper

Thyroglobulin (TG) gene was known to be regulated fat cell growth and differentiation and the endothelial differentiation sphingolipid G-protein-coupled receptor 1 (EDG1) gene involves blood vessel formation and known to be affecting carcass traits in beef cattle. The aim of this study was to identify the single nucleotide polymorphisms (SNPs) in both TG and EDG1 genes and to analyze the association with carcass traits in Korean cattle (Hanwoo). The T354C SNP in TG gene located at the 3' flanking region and c.-312A>G SNP located at 3'-UTR of EDG1 gene were used for genotyping the animals using PCR-RFLP method. Three genotypes were identified in T354C SNP in TG gene and only two AA and AG genotypes were observed for the c.-312A>G SNP in EDG1 gene. The results indicated that T354C SNP in TG gene was not significantly associated with carcass traits. However, the c.-312A>G SNP in EDG1 gene had significant effects on backfat thickness (BF) and yield index (YI). These results may provide valuable information for further candidate gene studies affecting carcass traits in Korean cattle and may use as marker assisted selection for improving the quality of meat in Hanwoo.

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문제 정의

Therefore, the aim of this study is to identify the polymorphisms in TG and EDG1 genes and to analyze their associations with carcass traits in Korean cattle, Hanwoo.

제안 방법

Seven carcass traits, namely raw weight (RW), backfat thickness (BF), longissimus muscle (LM), carcass weight (CW), yield index (YI), yield grade (YG), marbling score (MS), were used for association analysis of the SNPs in TG and EDG1 genes. The T354C SNP in TG gene was no association with the carcass traits.
Both carcass and meat quality data were provided by the Tobawoo. The meat parameter data including raw weight (RW), backfat thickness (BF), longissimus muscle (LM), carcass weight (CWT), yield index (YI), yield grade (YG), and marbling score (MS) were used in this study.
00 (Tom Hall, Ibis Therapeutics, California, USA). The sequence alignment was performed to determine the single nucleotide polymorphism (SNP) positions in the TG and EDG1 genes. The T354C SNP of TG gene was located in the 3’-UTR (Fig.

데이터처리

Pearson’s Chi-square test was used to verify the Hardy–Weinberg equilibrium status for the allele and genotype frequencies.
Pearson’s Chi-square test was used to verify the Hardy–Weinberg equilibrium status for the allele and genotype frequencies. The effects of TG and EDG1 genotypes on carcass traits were tested using ANOVA (Analysis of Variance) procedure in the SPSS version 17.0 program (SPSS, USA). The following model was used for the analysis the association of the genotype and carcass traits:

이론/모형

In order to test the pairwise differences between the genotypes, Tukey’s test was also performed.
The Pearson’s Chi-square test was also used to verify the Hardy–Weinberg equilibrium status.

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연합인증

한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석
Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo) 원문보기

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한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석 Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo) 원문보기

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한우에서 TG와 EDG1 유전자의 단일염기다형 확인 및 도체형질과의 연관성 분석
Identification of SNPs in TG and EDG1 genes and their relationships with carcass traits in Korean cattle (Hanwoo) 원문보기

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