[논문]Effect of Single Nucleotide Polymorphism of Endothelial Differentiation G-Protein Coupled Receptor 1 (EDG1) Gene on Marbling Score in Hanwoo

Shin, Sung-Chul; Chung, Eui-Ryong

doi:10.5851/kosfa.2012.32.6.776

Effect of Single Nucleotide Polymorphism of Endothelial Differentiation G-Protein Coupled Receptor 1 (EDG1) Gene on Marbling Score in Hanwoo 원문보기

Korean journal for food science of animal resources = 한국축산식품학회지, v.32 no.6, 2012년, pp.776 - 782

Shin, Sung-Chul (Division of Animal Science and Resources, Sangji University) , Chung, Eui-Ryong (Division of Animal Science and Resources, Sangji University)

Abstract ▼ AI-Helper

Marbling (intramuscular fat) is the most economically important meat quality trait in Hanwoo (Korean cattle). The endothelial differentiation G-protein coupled receptor 1 (EDG1) gene, involved in blood vessel formation, is located within the genomic region of a quantitative trait locus (QTL) for marbling on bovine chromosome 3. Thus, the EDG1 gene can be considered as a positional and functional candidate gene for meat quality in beef cattle. This study aimed to identify single nucleotide polymorphisms (SNPs) in the EDG1 gene and to evaluate their associations with carcass traits in Hanwoo population. We have sequenced a fragment of 5'-UTR of the EDG1 gene and identified one SNP. Genotyping of the g.166A>G SNP marker was carried out using PCR-RFLP analysis in 309 Hanwoo steers in order to evaluate their association with carcass traits. The g.166A>G SNP marker showed a significant effect on the marbling score. Animals with the GG genotype had higher marbling score compared with AA and AG genotypes (p<0.05). This SNP marker also showed a significant additive effects for the marbling score (p<0.05). These results suggest that the EDG1 gene can be used as a molecular marker for DNA marker-assisted selection in order to increase the levels of the marbling score in Hanwoo.

주제어

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문제 정의

In view of the lack of information regarded potential uses of molecular markers for marbling in Hanwoo, the objective of this study was to identify SNP marker in the EDG1 gene and to evaluate the effect of the EDG1 marker gene on carcass traits in Hanwoo population.
The animals with homozygous genotype GG showed a significantly higher marbling score compared with those of heterozygous genotype AG or homozygous genotype AA. The results of this study contribute to the information available on genetic markers for meat quality, especially intramuscular fat content. Therefore, the g.

제안 방법

The BFT and LMA were measured between the 12th and 13th rib interface. The MS was scored on a scale from 1 (worst) to 7 (best) with a cross section of the longissimus muscle at the 12th to 13th rib interface according to the Korean Beef Marbling Standard. The number of records, overall means and standard deviations (SD) of phenotypic values are shown in Table 1.
The PCR reaction was performed in a 20 μL reaction mixture containing 10 pM of each primer, 1.5 mM MgCl2, 2.5 mM of each dNTP and 1.5 unit of Taq DNA polymerase (Gibco BRL, Grand Island, NY), 10X reaction buffer and 50 ng of genomic DNA as template.
The genotype frequencies were as follows: 33.9% AA, 50.2% AG, and 15.9% GG for the g.166A>G SNP, and observed genotype distributions were in good agreement with those expected according to the Hardy-Weinberg equilibrium in this population.

대상 데이터

Positive clones were sequenced using an automated DNA sequencer (ABI 3130XL, Perkin-Elmer, USA) with Big-Dye 3.1 reagents.

데이터처리

Significant differences among mean values of different genotypes were calculated using Duncan's multiple range test, and determined to be statistically significant at p<0.05.
Allele and genotype frequencies of SNPs and Hardy-Weinberg equilibrium were estimated and tested using PROC ALLELE (SAS Inst. Inc., NC). Associations between SNP genotypes of the EDG1 gene and carcass traits were analyzed with the following liner mixed model using the MIXED procedure of SAS.

이론/모형

, NC). Associations between SNP genotypes of the EDG1 gene and carcass traits were analyzed with the following liner mixed model using the MIXED procedure of SAS.
Genotyping of the SNP located at position g.166A>G within the 5'-UTR of the EDG1 gene was performed by a PCR-RFLP method (Fig. 1).
The additive and dominance effects were also estimated using REG procedure of SAS version 9.2, where the additive effect was denoted as -1, 0, and 1 for AA, AG, and GG, respectively, and the dominance effect was denoted as 1, -2, and 1 for AA, AG, and GG, respectively.
g.166A>G SNP (numbering according to GenBank accession no. NC_007301.5, from Btau4.6.1) within the 5'-UTR of the EDG1 gene was performed by a PCR-RFLP (restriction fragment length polymorphism) method.

성능/효과

Therefore, EDG1 gene-specific SNP identified in this study may be a potential candidate DNA marker for marbling of Hanwoo. In conclusion, we have demonstrated that the SNP marker of EDG1 gene is significantly associated with marbling score and will be useful for effective marker-assisted selection to increase the levels of marbling in Hanwoo. Further studies are will be needed to confirm the associated effect on other populations and to detect additional SNP markers of EDG1 gene in Hanwoo.
166A>G marker but not for other measured traits. The animals with homozygous genotype GG showed a significantly higher marbling score compared with those of heterozygous genotype AG or homozygous genotype AA. The results of this study contribute to the information available on genetic markers for meat quality, especially intramuscular fat content.

후속연구

In conclusion, we have demonstrated that the SNP marker of EDG1 gene is significantly associated with marbling score and will be useful for effective marker-assisted selection to increase the levels of marbling in Hanwoo. Further studies are will be needed to confirm the associated effect on other populations and to detect additional SNP markers of EDG1 gene in Hanwoo.

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