[논문]Effects of pH and Carbon Sources on Biohydrogen Production by Co-Culture of Clostridium butyricum and Rhodobacter sphaeroides

Lee, Jung-Yeol; Chen, Xue-Jiao; Lee, Eun-Jung; Min, Kyung-Sok

doi:10.4014/jmb.1108.08009

Effects of pH and Carbon Sources on Biohydrogen Production by Co-Culture of Clostridium butyricum and Rhodobacter sphaeroides 원문보기

Journal of microbiology and biotechnology, v.22 no.3, 2012년, pp.400 - 406

Lee, Jung-Yeol (Department of Environmental Engineering, Kyungpook National University) , Chen, Xue-Jiao (Department of Environmental Engineering, Kyungpook National University) , Lee, Eun-Jung (Department of Environmental Engineering, Kyungpook National University) , Min, Kyung-Sok (Department of Environmental Engineering, Kyungpook National University)

Abstract ▼ AI-Helper

To improve the hydrogen yield from biological fermentation of organic wastewater, a co-culture system of dark- and photo-fermentation bacteria was investigated. In a pure-culture system of the dark-fermentation bacterium Clostridium butyricum, a pH of 6.25 was found to be optimal, resulting in a hydrogen production rate of 18.7 ml-$H_2/l/h$. On the other hand, the photosynthetic bacterium Rhodobacter sphaeroides could produce the most hydrogen at 1.81mol-$H_2/mol$-glucose at pH 7.0. The maximum specific growth rate of R. sphaeroides was determined to be 2.93 $h^{-1}$ when acetic acid was used as the carbon source, a result that was significantly higher than that obtained using either glucose or a mixture of volatile fatty acids (VFAs). Acetic acid best supported R. sphaeroides cell growth but not hydrogen production. In the co-culture system with glucose, hydrogen could be steadily produced without any lag phase. There were distinguishable inflection points in a plot of accumulated hydrogen over time, resulting from the dynamic production or consumption of VFAs by the interaction between the dark- and photo-fermentation bacteria. Lastly, the hydrogen production rate of a repeated fed-batch run was 15.9 ml-$H_2/l/h$, which was achievable in a sustainable manner.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

Therefore, we examined hydrogen production by pure or co-cultures of dark- and photo-fermentation bacteria after investigating the effects of pH and carbon sources on the hydrogen production of each system in batch tests. An evaluation of a repeated fed-batch run of the co-culture system based on continuous hydrogen production and fermentation outlet composition was performed.
During acidogenic hydrogenesis of sugars, various kinds of metabolites are known to be generated along with evolved hydrogen [11]. In this study, the main metabolites of the dark-fermentation process were found to be acetic and butyric acids (Fig. 2), but other VFAs such as lactic and propionic acids were not detected by our analytical method. It is interesting to note that butyric acid was present at a very low concentration, below 700 mg/l, at pH 5.
sphaeroides. Then, acetate or glucose was used as the sole carbon source for photo fermentation at pH 6.25 in order to investigate the effects of various substrates on hydrogen production. The photo-fermentation experiment was carried out under an illumination of 5,000 lux and 30℃ after the replacement of the gas phase in the reactor with argon [21].

성능/효과

C. butyricum was cultivated at 30℃ for 7 h in PYG medium (pH 6.5), which was composed of K2HPO4 (0.9 g/l), KH2PO4 (0.9 g/l), NaCl (0.9 g/l), (NH4)SO4 (0.9 g/l), MgSO4 (0.09 g/l), CaCl2 (0.09 g/l), peptone (10 g/l), yeast extract (5 g/l), cysteine·HCl (0.5 g/l), Na2CO3·10H2O (4.0 g/l), aminobenzoic acid (100 µl/l), and glucose (10 g/l).
In conclusion, the optimum pH was 6.25 in the C. butyricum pure-culture system, whereas neutral pH was preferable for the production of hydrogen by a pure culture of the photo-fermentation bacterium R. sphaeroides. The results presented on this research show that a pH of 6.
sphaeroides. The maximum specific growth rates of this photo-fermentation bacterium were determined to be 0.25, 0.37, and 2.93 h^-1 when using 6 g/l VFAs, 10 g/l glucose, and 3 g/l acetic acid, respectively, indicating that acetic acid best supported R. sphaeroides cell growth. When considering hydrogen production, however, acetic acid as a single substrate was not sufficient since it resulted in the lowest hydrogen yield in this study.
sphaeroides. The results presented on this research show that a pH of 6.25 in the co-culture system is reasonable, considering not only enhanced hydrogen production but the mitigation of methanogenic hindrance.

후속연구

This was the result of the dynamic production of VFAs by the dark-fermentation bacterium and the simultaneous consumption of VFAs by the photo-fermentation bacterium. Further study about dynamic changes between substrate and intermediate products during fermentation is needed. Lastly, the repeated fed-batch run of the co-culture system clearly showed the possibility of sustainable hydrogen production.

참고문헌 (21)

Afgan, N. H. and M. G. Carvalho. 2004. Sustainability assessment of hydrogen energy systems. Int. J. Hydrogen Energy 29: 1327-1342.

상세보기
Alalayah, W. M., M. S. Kalil, A. A. H. Kadhum, J. M. Jahim, and N. M. Alauj. 2008. Hydrogen production using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). Int. J. Hydrogen Energy 33: 7392-7396.

상세보기
Clesceri, L. S., A. E. Greenberg, and A. D. Eaton (Eds.) 1998. Standard Methods for the Examination of Water and Wastewater, 20th Ed. American Public Health Association, Washington, DC.
Barbosa, M. J., J. M. S. Rocha, J. Tramper, and R. H. Wifjjels. 2001. Acetate as a carbon source for hydrogen production by photosynthetic bacteria. J. Biotechnol. 85: 25-33.

상세보기
Brosseau, J. D. and J. E. Zajic. 1982. Hydrogen-gas production with Citrobacter intermedius and Clostridium pasteurianum. J. Chem. Technol. Biotechnol. 32: 496-502.

상세보기
Chen, C. C., C. Y. Lin, and J. S. Chang. 2001. Kinetics of hydrogen production with continuous anaerobic cultures utilizing sucrose as the limiting substrate. Appl. Microbiol. Biotechnol. 57: 56-64.

상세보기
Demirel, B., P. Scherer, O. Yenigun, and T. T. Onay. 2010. Production of methane and hydrogen from biomass through conventional and high-rate anaerobic digestion processes. Crit. Rev. Environ. Sci. Technol. 40: 116-146.

상세보기
Fang, H. H. P. and H. Liu. 2002. Effect of pH on hydrogen production from glucose by a mixed culture. Bioresour. Technol. 82: 87-93.

상세보기
Fang, H. H. P., H. Zhu, and T. Zhang. 2006. Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides. Int. J. Hydrogen Energy 31: 2223-2230.

상세보기
Fascetti, E., E. D'Addario, O. Todini, and A. Robertiello. 1998. Photosynthetic hydrogen evolution with volatile organic acids derived from the fermentation of source selected municipal solid wastes. Int. J. Hydrogen Energy 23: 753-760.

상세보기
Jo, J. H., D. S. Lee, D. Park, and J. M. Park. 2008. Biological hydrogen production by immobilized cells of Clostridium tyrobutyricum JM1 isolated from a food waste treatment process. Bioresour. Technol. 99: 6666-6672.

상세보기
Kapdan, I. K. and F. Kargi. 2006. Bio-hydrogen production from waste materials. Enzyme Microb. Technol. 38: 569-582.

상세보기
Kraemer, J. T. and D. M. Bagley. 2007. Improving the yield from fermentative hydrogen production. Biotechnol. Lett. 29: 685-695.

상세보기
Kim, J. O., Y. H. Kim, J. Y. Ryu, B. K. Song, I. H. Kim, and S. H. Yeom. 2005. Immobilization methods for continuous hydrogen gas production biofilm formation versus granulation. Process Biochem. 40: 1331-1337.

상세보기
Manish, S. and R. Banerjee. 2008. Comparison of biohydrogen production processes. Int. J. Hydrogen Energy 33: 279-286.

상세보기
Odom, J. M. and J. D. Wall. 1983. Photoproduction of $H_2$ from cellulose by an anaerobic bacterial coculture. Appl. Environ. Microbiol. 45: 1300-1305.

상세보기
Shi, X. Y. and H. Q. Yu. 2006. Continuous production of hydrogen from mixed volatile fatty acids with Rhodopseudomonas capsulata. Int. J. Hydrogen Energy 31: 1641-1647.

상세보기
Taguchi, F., N. Mizukami, T. Saito-Taki, and K. Hasegawa. 1995. Hydrogen production from continuous fermentation of xylose during growth of Clostridium sp. strain No. 2. Can. J. Microbiol. 41: 536-540.

상세보기
Uyar, B., I. Eroglu, M. Yucel, and U. Gunduz. 2009. Photofermentative hydrogen production from volatile fatty acids present in dark fermentation effluents. Int. J. Hydrogen Energy 34: 4517-4523.

상세보기
Van Andel, J. G., G. R. Zoutberg, P. M. Crabbendam, and A. M. Breure. 1985. Glucose fermentation by Clostridium butyricum grown under a self-generated gas atmosphere in chemostat culture. Appl. Microbiol. Biotechnol. 23: 21-26.

상세보기
Yokoi, H., S. Mori, J. Hirose, S. Hayashi, and Y. Takasaki. 1998. $H_2$ production from starch by a mixed culture of Clostridium butyricum and Rhodobacter sp. M-19. Biotechnol. Lett. 20: 895-899.

상세보기

저자의 다른 논문 :

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증